l-Carnitine protects against testicular dysfunction caused by gamma irradiation in mice

Ahmed, Mohamed M.; Ibrahim, Zein Shaban; Alkafafy, Mohamed; El-Shazly, Samir Ahmed

doi:10.1016/j.acthis.2014.04.010

Cited by 34 publications

(27 citation statements)

References 46 publications

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“…The body can endogenously synthesize about 10% of the L-carnitine it requires from lysine and methionine amino acids in the skeletal muscle, heart, liver, kidney, and brain; the remaining amount is generally obtained through diet 12,13 . Experimental models have demonstrated its radioprotective effects in the kidney, lens and cochlea, retina, brain, growing bone, testes, oral mucosa, small intestine and bone marrow [14][15][16][17][18][19][20][21] .…”

Section: Introductionmentioning

confidence: 99%

L-carnitine has a protective effect on the colonic mucosa during abdominopelvic radiotherapy in rats

et al. 2016

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PURPOSE:To evaluate histopathologically the radioprotective effect of L-carnitine on the colonic mucosa in rats undergoing abdominopelvic irradiation. METHODS:Thirty-two rats were randomly assigned to four experimental groups: intraperitoneal administration of normal saline (group 1) or L-carnitine (300 mL/kg; group 2), followed in groups 3 and 4, respectively, by one dose of abdominopelvic radiation (20 Gy) 30 min later. Rats were sacrificed 5 days after radiation, and their descending colons were resected for histopathological evaluation of the presence and severity of damage. RESULTS:Average damage scores did not differ significantly between groups 1 and 2 (0.13 ± 0.35 and 0.25 ± 0.46, respectively); the group 3 score was highest (10.25 ± 0.71), and the group 4 score (3.63 ± 1.41) was significantly lower than that of group 3 (both p = 0.0001). Pre-radiation L-carnitine administration significantly reduced mucosal thinning, crypt distortion, reactive atypia, inflammation, cryptitis, and reactive lymph-node hyperplasia (all p < 0.01).CONCLUSIONS: L-carnitine had a radioprotective effect on rat colonic mucosa. L-carnitine use should be explored for patients with gastrointestinal cancer, who have reduced serum L-carnitine levels.

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Section: Introductionmentioning

confidence: 99%

L-carnitine has a protective effect on the colonic mucosa during abdominopelvic radiotherapy in rats

et al. 2016

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“…Total RNA was extracted from 100 mg of each tissue sample using QIAzol lysis reagent (QIAGEN Inc., Valencia, CA) according to the manufacturer's instructions and as detailed previously. 26) Integrity of the prepared RNA was checked by electrophoresis. RNA concentration and purity were determined spectrophotometrically at 260 and 280 nm.…”

Section: )mentioning

confidence: 99%

Anti-obesity effects of Taif and Egyptian pomegranates: molecular study

Ahmed

Samir

El-Shehawi

et al. 2015

Bioscience, Biotechnology, and Biochemistry

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The present study investigated the anti-obesity effects of pomegranate (Punica granatum) juices from the two Saudi Arabian, Taif red, Taif white, and Egyptian pomegranates in high-fat diet (HFD)-induced obese rats. Administrating any of the used juices decreased the body weight gain, food consumption, and serum levels of lipid, leptin, and glucose, while it increased serum insulin level. Histologically, all types of juices decreased the number and size of lipid droplets in hepatocytes compared to the obese, non-treated animals. All juices types upregulated the hepatic mRNA expression of hormone-sensitive lipase, pyruvate kinase, and adiponectin in obese rats; the genes were all suppressed by HFD feeding. Additionally, the expression of fatty acid synthase, sterol regulatory element-binding protein-1c, and acetyl-CoA carboxylase1 was also upregulated by all types of juices. Conversely, ghrelin mRNA expression was downregulated by all used juices’ types. These findings demonstrate that all types of tested juices protect against the HFD-induced obesity in rats.

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“…15 Integrity of the prepared RNA was checked by electrophoresis, while RNA concentration and purity were determined spectrophotometrically at 260 nm and 280 nm. For cDNA synthesis, 2 µg RNA were reverse transcribed with oligo-dT primer and Moloney murine leukaemia virus (M-MuLV) reverse transcriptase (SibEnzyme Ltd. AK, Novosibirsk, Russia) as previous described.…”

Section: Rna Extraction and Cdna Synthesismentioning

confidence: 99%

“…For cDNA synthesis, 2 µg RNA were reverse transcribed with oligo-dT primer and Moloney murine leukaemia virus (M-MuLV) reverse transcriptase (SibEnzyme Ltd. AK, Novosibirsk, Russia) as previous described. 15 The resultant cDNA was preserved at -20°C until used.…”

Section: Rna Extraction and Cdna Synthesismentioning

confidence: 99%

Synthesis, characterization, and efficacy evaluation of a new anti-diabetic vanadyl(II) thiamine hydrochloride complex in streptozotocin-induced diabetic rats

El-Shazly

Ahmed

Ibrahim

et al. 2015

Int J Immunopathol Pharmacol

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Diabetes mellitus (DM) is a chronic metabolic disorder characterized by hyperglycemia due to abnormalities in either insulin secretion or action. A range of vanadium complexes have been synthesized and demonstrated to be effective in lowering hyperglycemia. Thiamine administration was also reported to prevent deterioration in fasting glucose and insulin levels, and to improve glucose tolerance in hyperglycemic patients. This study has been conducted to evaluate the ionic vanadyl(II) thiamine hydrochloride complex (VC) as a new anti-diabetic candidate. The new complex was characterized by infrared spectroscopy (FT-IR), electronic spectra, magnetic susceptibility, electron spin resonance (ESR), scanning electron microscopy (SEM), and thermogravimetric analysis (TGA). The anti-diabetic effect of VC was investigated in comparison to vanadium sulfate in streptozotocin (STZ)-induced diabetic rats. Treatment of diabetic rats with VC versus vanadyl sulfate showed a more potent effect on reducing serum glucose and cholesterol close to normal levels. VC suppressed the diabetes-induced upregulation of hepatic glucose transporter (GLUT)-2, Phosphoenol pyruvate carboxykinase (PEPCK), and hormone-sensitive lipase (HSL) more significantly than vanadyl sulfate. Either vanadyl sulfate or VC restored hepatic sterol regulatory element-binding protein transcription factor-1c (SREBP-1c) and muscle hexokinase (HK) mRNA expression that was downregulated in diabetic group. Pyruvate kinase (PK) mRNA expression was restored more significantly in VC-treated than vanadyl sulfate-treated diabetic rats. These results indicate that the newly synthesized VC could be an effective anti-diabetic candidate as the anti-diabetic activity of the ionic vanadium was enhanced after being modified with the organic ligand, thiamin. The results also suggest that VC achieves its effect most likely through modulating the transcription of energy metabolizing enzymes.

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l-Carnitine protects against testicular dysfunction caused by gamma irradiation in mice

Cited by 34 publications

References 46 publications

L-carnitine has a protective effect on the colonic mucosa during abdominopelvic radiotherapy in rats

L-carnitine has a protective effect on the colonic mucosa during abdominopelvic radiotherapy in rats

Anti-obesity effects of Taif and Egyptian pomegranates: molecular study

Synthesis, characterization, and efficacy evaluation of a new anti-diabetic vanadyl(II) thiamine hydrochloride complex in streptozotocin-induced diabetic rats

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