L-cysteine modified silver nanoparticles for selective and sensitive colorimetric detection of vitamin B1 in food and water samples

Abstract

The use of L-cysteine modified silver nanoparticles (Cys-capped AgNPs) as a colorimetric probe for determination of vitamin B1 (thiamine) is described in the present work. This method is based on the measurement of red shift of localized surface plasmon resonance (LSPR) band of Cys-capped AgNPs in the region of 200-800 nm. The color of Cys-capped AgNPs was changed from yellow to colorless by the addition of vitamin B1. The mechanism for detection of vitamin B1 is based on the electrostatic interaction between positively charged vitamin B1, which causes the red shift of LSPR band from 390 nm to 580 nm. The interaction between Cys-capped AgNPs and vitamin B1 was theoretically explored by density function theory (DFT) using LANL2DZ basis sets with help of Gaussian 09 (C.01) program. The morphology, size distribution and optical properties of Cys-capped AgNPs were characterized by transmission electron microscope (TEM), UV-Visible spectrophotometry, Fourier transform infrared spectroscopy (FTIR) and dynamic light scattering (DLS) techniques. The method is linear in the range of 25-500 μg mL À1 with correlation coefficient (R 2) 0.992 and limit of detection of 7.0 μg mL À1. The advantages of using Cys-capped AgNPs as a chemical sensor in colorimetry assay are being simple, low cost and selective for detection of vitamin B1 from food (peas, grapes and tomato) and environmental (river, sewage and pond) water samples.

Recent progress in 0D optical nanoprobes for applications in the sensing of (bio)analytes with the prospect of global health monitoring and detailed mechanistic insights

“…Recently, AgNPs have been widely explored for biosensing applications towards the detection of various bio-analytes, such as dopamine, cysteamine, cysteine, glucose, lactate, uric acid, alkaline phosphatase, vitamin, adenosine, H 2 O 2 etc . 171–199 (Table 5). AgNPs exhibit promising affinity towards thiol functionalities.…”

“…202 Deb et al reported the use of l -cysteine-functionalized AgNPs for the sensitive chromogenic detection of thiamine. 177 The electrostatic interaction with the positively charged thiamine is the driving force for the thiamine-induced aggregation of the nanosensor, which ultimately led to a bathochromic shift in the LSPR band from 390 nm to 580 nm with a naked eye chromogenic change from yellow to colorless (Fig. 11B).…”

LSPR‐based colorimetric biosensing for food quality and safety

“…They found that the AuNPs-B 12 RNA aptamer complex aggregated when it trapped vitamin B 12 , yielding a large bulky complex, which altered the Au surface LSPR wave that partially enhanced the fluorescence ability of RNA under UV-Vis incident light and red-to-purple color change in real samples. Likewise, colorimetric detection of vitamin B 1 (thiamine) was reported using l-cysteine modified AgNPs, as solution changed from yellow to colorless in the presence of vitamin B 1 in food (peas, grapes, and tomato) and environmental water samples (Khalkho et al, 2020).…”

Advances in electrochemical and optical sensing techniques for vitamins detection: a review

“…Optical sensing, too, has gathered a lot of attention. Recent research could find colorimetric methods being used to determine Vit D (Lee et al 2017;Keyfi et al 2018), Vit C (Kalaiyarasan and Joseph May 2019), and B1 (Duenchay et al 2020). Surface plasmon resonance (SPR) and localized surface plasmon resonance (LSPR)-based sensing have also generated a lot of interest for Vit A (Prakashan et al 2019), Vit D (Jo et al 2021), Vit C (Zhu et al 2020a), and Vit B3 (Verma and Gupta 2013) detection.…”