L-forms of group A streptococci Size of viable particles and growth requirements

Boven, C. P. A. van; Ensering, Henriette L.; Hijmans, W.; Kastelein, Margaretha J. W.

doi:10.1007/bf02097512

Cited by 1 publication

(2 citation statements)

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“…Williams found no effect due to a filter-paper covering on colony formation by an L-phase variant of S. aureus (35). van Boven and Hijams et al (3,4) reported that, dependent upon the experimental conditions, the filtration end point of group A streptococcal L-phase variants AED and GL8 varied between 0.25 and 0.45 ,gm, but that only bodies greater than 0.8 um in diameter could develop into colonies. Examina-tion of the 0.45-,tm filtrate, concentrated in an ultracentrifuge, revealed elements ranging in size from 0.7 to 1.7 Mm.…”

Section: Resultsmentioning

confidence: 96%

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Growth of Streptococcal Protoplasts and L-Colonies on Membrane Filters

Wyrick

Gooder

1971

J Bacteriol

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Membrane filters (Millipore Corp.; pore sizes 1.2 to 0.22 Mm) were placed on the surface of L-phase growth medium solidified with agar. The filter and the surrounding medium were inoculated with either protoplasts or stable broth-grown Lphase variants obtained from Streptococcus faecium strain F24. The L-phase inoculum gave rise to viable L-colonies on the filters and on the medium, whereas protoplasts gave colony formation only on the medium. However, when the Millipore filters were covered by a layer of solid L-phase medium, 75 Mm or greater in depth, before inoculation with protoplasts, colony formation resulted but with atypical morphology. In contrast, inoculation of protoplasts on Nuclepore and Sartorius membrane filters did give rise to L-colonies on the surface and underneath the filters after 2 days of incubation at 37 C. Submicroscopic, viable L-phase elements produced during colony formation were capable of passing through membrane filters with pore channels as small as 0.22 Mrm; these elements required transfer from underneath the filters to fresh agar medium in order to develop into L-phase colonies. Membrane filters were also placed on the surface of L-phase growth medium solidified with gelatin. Inoculation of the filters and surrounding medium with a lysozyme-prepared protoplast suspension gave rise to streptococci on the surface of the filters and on the medium. However, inoculation with the stable broth-grown Lphase variants gave rise to atypical colonies on the medium and only small patches of abortive growth on the filters.

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Section: Resultsmentioning

confidence: 96%

“…Filters were chosen of a pore size which would retard the parent bacterial cell. Growth of group A streptococcal L-phase var-646 iants has been observed to occur through filters with pore sizes as small as 0.45 Mm (3)(4)(5)31), 0.22 um (34), and 0.10 gm (9).…”

mentioning

confidence: 99%

Growth of Streptococcal Protoplasts and L-Colonies on Membrane Filters

Wyrick

Gooder

1971

J Bacteriol

View full text Add to dashboard Cite

show abstract

L-forms of group A streptococci Size of viable particles and growth requirements

Cited by 1 publication

References 3 publications

Growth of Streptococcal Protoplasts and L-Colonies on Membrane Filters

Growth of Streptococcal Protoplasts and L-Colonies on Membrane Filters

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