l-Guluronan-specific alginate lyase from a marine bacterium associated with Sargassum

Brown, Bette Jo; Preston, James F.

doi:10.1016/0008-6215(91)84148-8

Cited by 42 publications

(15 citation statements)

References 29 publications

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“…Un fenómeno similar ocurre con la expresión de la alginatoliasa, la cual puede ser inducida cuando son cultivadas en presencia de alginato, como en Alginovibrio aquatilis (Stevens & Levin, 1977) y otras bacterias marinas como algunas asociadas con Sargassum (Doubet & Quatrano, 1984;Romeo & Preston, 1986;Brown & Preston, 1991). Sin embargo, en algunas especies, como por ejemplo Pseudomonas alginovora, la producción de alginatoliasa es constitutiva (Boyen et al, 1990).…”

Section: Discussionunclassified

Cepa antártica de Bacillus sp., con actividad extracelular de tipo agarolítica y alginatoliasa

et al. 2013

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RESUMENDiversas bacterias asociadas a macroalgas pueden usar fi cocoloides como fuente de carbono. Las bacterias de la Antártica, poseen características fi siológicas adaptativas que habrían evolucionado para permitir su supervivencia y funcionamiento dentro de las condiciones adversas de ese ecosistema. Por lo tanto, bacterias aisladas desde algas antárticas podrían tener la capacidad de degradar enzimáticamente azúcares complejos a temperaturas inferiores que aquellas aisladas desde zonas más cálidas, lo que potencialmente podría tener aplicaciones en la mejora en procesos industriales que utilizan enzimas. El análisis del gen ribosomal 16S indica que la cepa bacteriana aislada desde algas varadas en la Isla Rey Jorge (Antártica), pertenece al género Bacillus. El sobrenadante libre de células presentó actividad agarasa y alginatoliasa. Se encontraron diferencias signifi cativas en la temperatura óptima para hidrolizar agarosa y alginato, evaluada dentro de un rango de 4 a 30 °C. La actividad agarolítica fue mayor a 4 °C, mientras que la actividad alginatoliasa fue mayor a 30 °C. Estos resultados poseen un alto valor biotecnológico y podría ser utilizada con fi nes industriales. PALABRAS CLAVE:Bacillus, Antártica, Agarasa, Alginatoliasa. ABSTRACTSeveral bacteria associated to macroalgae can use phycocolloids as carbon source. Antarctic Bacteria's have physiological characteristics that might have evolved to allow the survival and functioning under the harsh conditions of that ecosystem. Therefore, Antarctic bacteria isolated from algae should have the ability to degrade complex sugars at lower temperatures than those isolated from warmer areas, which may have applications in the improvement of industrial processes that uses enzymes. The bacterial strain isolated from wrack algae, in King George Island, Antarctica, was identifi ed as Bacillus on the base of 16S ribosomal gene analysis. The cell-free supernatant of the culture medium showed alginate-lyase and agarase activities. Signifi cant differences in the optimal temperature to hydrolyze agarose and alginate were found within a range of 4 to 30 °C. While the agarase activity was higher at 4° C, the alginate-lyase activity was higher at 30 °C. Our results have biotechnological value and could be used with industrial aims.

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Section: Discussionunclassified

Cepa antártica de Bacillus sp., con actividad extracelular de tipo agarolítica y alginatoliasa

et al. 2013

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“…echinoderm (Boyen et al, 1990;Brown and Preston, 1991;Larsen et al, 1993). Jacob (2014), menyebutkan sebagian besar protein dan karbohidrat pada rumput laut tidak dapat dicerna oleh non ruminant.…”

Section: ) Penanganan Dan Pengolahanunclassified

Review: Potensi, Faktor Pembatas dan Pengolahan Rumput Laut Coklat (Phaeophyceae) sebagai Pakan Ayam Petelur

Dewi

Yuniza

Nuraini

et al. 2018

JPI

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ABSTRAKTulisan ini dibuat untuk mengetahui potensi, faktor pembatas, dan pengolahan rumput laut coklat (Phaeophyceae) sebagai pakan ayam petelur. Rumput laut coklat (Phaeophyceae) adalah rumput laut yang mengandung pigmen klorofil a, karotin, xanthofil dan fukosantin, pigmen yang dominan adalah fukosantin yang menutupi warna lainnya dan menyebabkan rumput laut ini kelihatan berwarna coklat. Rumput laut coklat memiliki potensi sebagai bahan pakan ayam petelur karena ketersediaan yang melimpah, tidak bersaing dengan kebutuhan manusia, mengandung zat-zat nutrisi, tidak bersifat toksik, kaya asam lemak omega 3, mengandung pigmen fukosantin, alginat dan fukoidan. Faktor pembatas penggunaan rumput laut ini, yaitu 1) Kandungan nutrisi dan kualitas: alginat tinggi, protein rendah, dan energi rendah; 2) Kandungan anti nutrisi: kadar garam tinggi dan phlorotannin; 3) Penanganan dan pengolahan: membutuhkan berbagai pengolahan khusus sebelum digunakan sebagai pakan ternak. Faktor-faktor pembatas tersebut dapat diatasi dengan perlakuan fisik (pencucian, pengeringan, autoklaf, perebusan dan freezing), fermentasi dan penambahan enzim dalam pakan sebagai upaya untuk meningkatkan ketersediaan zat nutrisi dan mendetoksifikasi senyawa antinutrisi. Rumput laut coklat (Phaeophyceae) memiliki potensi sebagai bahan pakan penyusun ransum ayam petelur.

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“…This reaction leads to the formation of an unsaturated uronate at the newly generated, nonreducing end. Oligosaccharides from a degree of polymerization of 2 to 3 or 5 can be obtained by polysaccharide degradation with lyases (3,20,40). To date, lyase activities on various polymers have been described as alginate (16,32,35), gellan (41), hyaluronan (39), ulvan (25), and xanthan (15).…”

Section: ؉mentioning

confidence: 99%

Purification and Properties of a Glucuronan Lyase from Sinorhizobium meliloti M5N1CS (NCIMB 40472)

Costa

Michaud

Petit

et al. 2001

Appl Environ Microbiol

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A glucuronan lyase extracted from Sinorhizobium meliloti strain M5N1CS was purified to homogeneity by anion-exchange chromatography. The purified enzyme corresponds to a monomer with a molecular mass of 20 kDa and a pI of 4.9. A specific activity was found only for polyglucuronates leading to the production of 4,5-unsaturated oligoglucuronates. The enzyme activity was optimal at pH 6.5 and 50°C. Zn 2؉ , Cu 2؉ , and Hg 2؉(1 mM) inhibited the enzyme activity. No homology of the enzyme N-terminal amino acid sequence was found with any of the previously published protein sequences. This enzyme purified from S. meliloti strain M5N1CS corresponding to a new lyase was classified as an endopolyglucuronate lyase.Bacterial polysaccharides as well as plant polysaccharides can be degraded by specific glycosidases corresponding either to hydrolases or to lyases. Among the polyanionic polymers, some of them (i.e., alginate) are exclusively degraded by lyases (35,40,42). The lyase cleaving mechanism consists of a ␤-elimination, in which a general base-catalyzed abstraction of the proton at C-5 of a uronic acid occurs. A transfer of electrons from the carboxyl group to form a double bond between C-4 and C-5 results in the elimination of the 4-O-glycosidic bond and in the formation of 4-deoxy-L-erythro-hex-4-enopyranosyluronic acid. This reaction leads to the formation of an unsaturated uronate at the newly generated, nonreducing end. Oligosaccharides from a degree of polymerization of 2 to 3 or 5 can be obtained by polysaccharide degradation with lyases (3,20,40). To date, lyase activities on various polymers have been described as alginate (16,32,35), gellan (41), hyaluronan (39), ulvan (25), and xanthan (15). The tested lyases were found in many organisms such as marine gastropods (19) or fungi (36, 45) as well as in many bacteria (14,15,31) and bacteriophages (1, 4). The enzyme localization in the producing organisms may be in the cytosol (28, 36) and the periplasmic space (22), as for alginate lyase from Azotobacter species, or in extracellular fractions, as for a Bacillus circulans lyase (26). Lyases present possible applications in the medical field, such as, for example, the alginate lyase, which promotes diffusion of antibiotics through extracellular polymers produced by the pathogenic Pseudomonas sp. (17). Due to potential applications, studies concerning polysaccharide lyases have been increasing.The Sinorhizobium meliloti mutant strain M5N1CS (NCIMB 40472) (7), which induces the formation of effective nodules on alfalfa roots (12), produces a glucuronan (6, 18) corresponding to high-molecular-weight (HMW) and low-molecular-weight (LMW) (134)-␤-D-polyglucuronic acid partially acetylated at the C-2 and/or C-3 position. The detection of LMW glucuronan containing a 4-5 unsaturated glucuronic residue at the nonreducing end (29, 30) led us to suspect a polymer degradation by a glucuronan lyase.In order to produce oligoglucuronans, a lyase degrading various acetylated and nonacetylated glucuronans was purified and character...

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l-Guluronan-specific alginate lyase from a marine bacterium associated with Sargassum

Cited by 42 publications

References 29 publications

Cepa antártica de Bacillus sp., con actividad extracelular de tipo agarolítica y alginatoliasa

Cepa antártica de Bacillus sp., con actividad extracelular de tipo agarolítica y alginatoliasa

Review: Potensi, Faktor Pembatas dan Pengolahan Rumput Laut Coklat (Phaeophyceae) sebagai Pakan Ayam Petelur

Purification and Properties of a Glucuronan Lyase from Sinorhizobium meliloti M5N1CS (NCIMB 40472)

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