L-lactic Acid Production from Cassava Starch by Thermotolerant Rhizopus 
 microsporus LTH23

Yuwa-amornpitak, Thalisa; Chookietwattana, Kannika

doi:10.3923/jbs.2014.284.291

Cited by 6 publications

(7 citation statements)

References 15 publications

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“…Currently, glycerol is an abundant C-source derived from biodiesel industries that few microbes can use for lactic acid production. Cassava starch can easily be converted to lactic acid by R. microsporus LTH23, 6 but only limited lactic acid was produced from on glycerol media. We then developed mutant strains by UV and chemical treatments.…”

Section: Resultsmentioning

confidence: 99%

“…R. microsporus LTH23 was isolated from soil and identified by sequencing of the Internal transcript spacer (ITS) region compared with a previous study by Yuwa-amornpitak and Chookietwatana (2014). 6 The mutant strains of R. microsporus 2T5, R. microsporus 5T3, R. microsporus 10T5, and R. microsporus H120M4 were derived from the wild type R. microsporus LTH23 by UV and chemical treatments. The genomic sequence of R. microsporus LTH23 was submitted to NCBI gene bank under the accession number KU358721.…”

Section: Methodsmentioning

confidence: 99%

“…A sole isomer of l -lactic is generated by Rhizopus fungi. In addition, this fungus is capable of using a wide range of substrates for lactic acid production, including glucose, 5 molasses, starch, 6 lignocelluloses, 7 , 8 office paper, 9 and crude glycerol. 10 Currently, biodiesel is an important alternative fuel that is used in many counties in the world to replace petro oil.…”

Section: Introductionmentioning

confidence: 99%

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Bioconversion of waste cooking oil glycerol from cabbage extract to lactic acid by Rhizopus microsporus

Yuwa-amornpitak

Chookietwatana

2018

Brazilian Journal of Microbiology

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Glycerol from spent oil was processed by transesterification for biodiesel production. Although glycerol contains many types of impurities, it can be used as a C-source for lactic acid production by fungi, such as Rhizopus microsporus. In this study, we found that wild type R. microsporus (LTH23) produced more lactic acid than the mutant strains on cabbage glycerol media (CG media). More lactic acid was produced on CG media than on cabbage extract media (C media) by about two-fold in batch fermentation conditions. In addition, we found that lactic acid production in a fed-batch process was also slightly higher than in a batch process. To study the combined effects of pH, urea, and glycerol waste concentration on lactic acid production, a response surface methodology was used. The optimum pH, urea, and glycerol waste concentrations were pH 6.5, 3.75g/L, and 17g/L, respectively. The maximum lactic acid production predicted by this equation model was 4.03g/L.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bioconversion of waste cooking oil glycerol from cabbage extract to lactic acid by Rhizopus microsporus

Yuwa-amornpitak

Chookietwatana

2018

Brazilian Journal of Microbiology

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“…A few examples about the use of fungi for the direct conversion of starch to LA are also listed in Table . Among them, the best results were obtained by Yuwa‐amornpitak and Chookietwattana with Rhizopus microsporus reaching a concentration of 56 g L −1 pure L‐LA and a productivity of 1.2 g L −1 h −1 . In comparison, the process described in this study for the direct production of LA with G. stearothermophilus has a productivity of 1.8 g L −1 h −1 , which is the second best only after Zhang and Cheryan and has the economic and technical advantage of requiring no sterilization and of being run under non‐sterile conditions throughout the whole process.…”

Section: Resultsmentioning

confidence: 99%

Direct fermentation of potato starch and potato residues to lactic acid by Geobacillus stearothermophilus under non‐sterile conditions

Smerilli

Wurz

Haas

et al. 2015

J of Chemical Tech & Biotech

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BACKGROUNDLactic acid is an important biorefinery platform chemical. The use of thermophilic amylolytic microorganisms to produce lactic acid by fermentation constitutes an efficient strategy to reduce operating costs, including raw materials and sterilization costs.RESULTSA process for the thermophilic production of lactic acid by Geobacillus stearothermophilus directly from potato starch was characterized and optimized. Geobacillus stearothermophilus DSM 494 was selected out of 12 strains screened for amylolytic activity and the ability to form lactic acid as the major product of the anaerobic metabolism. In total more than 30 batches at 3–l scale were run at 60 °C under non-sterile conditions. The process developed produced 37 g L−1 optically pure (98%) L-lactic acid in 20 h from 50 g L−1 raw potato starch. As co-metabolites smaller amounts (<7% w/v) of acetate, formate and ethanol were formed. Yields of lactic acid increased from 66% to 81% when potato residues from food processing were used as a starchy substrate in place of raw potato starch.CONCLUSIONSPotato starch and residues were successfully converted to lactic acid by G. stearothermophilus. The process described in this study provides major benefits in industrial applications and for the valorization of starch-rich waste streams. © 2015 The Authors.Journal of Chemical Technology & Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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“…As it has been shown that the choice of nitrogen source (N source) can have a significant impact on carboxylic acid production in different fungi species (Zhang, Jin, and Kelly, 2007;Yuwa-Amompitak and Chookietwattana, 2014;Guo et al, 2021) we examined whether this would also be the case for O. polymorpha. The original N source in the chosen Verduyn medium is ammonium sulfate ((NH 4 ) 2 SO 4 ) (Verduyn et al, 1992).…”

Section: Impact Of Nitrogen Sourcesmentioning

confidence: 99%

Engineering the methylotrophic yeast Ogataea polymorpha for lactate production from methanol

Wefelmeier,

Schmitz,

Haut

et al. 2023

Front. Bioeng. Biotechnol.

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Introduction: Lactate has gained increasing attention as a platform chemical, particularly for the production of the bioplastic poly-lactic acid (PLA). While current microbial lactate production processes primarily rely on the use of sugars as carbon sources, it is possible to envision a future where lactate can be produced from sustainable, non-food substrates. Methanol could be such a potential substrate, as it can be produced by (electro)chemical hydrogenation from CO2.Methods: In this study, the use of the methylotrophic yeast Ogataea polymorpha as a host organism for lactate production from methanol was explored. To enable lactate production in Ogataea polymorpha, four different lactate dehydrogenases were expressed under the control of the methanol-inducible MOX promoter. The L-lactate dehydrogenase of Lactobacillus helveticus performed well in the yeast, and the lactate production of this engineered strain could additionally be improved by conducting methanol fed-batch experiments in shake flasks. Further, the impact of different nitrogen sources and the resulting pH levels on production was examined more closely. In order to increase methanol assimilation of the lactate-producing strain, an adaptive laboratory evolution experiment was performed.Results and Discussion: The growth rate of the lactate-producing strain on methanol was increased by 55%, while at the same time lactate production was preserved. The highest lactate titer of 3.8 g/L in this study was obtained by cultivating this evolved strain in a methanol fed-batch experiment in shake flasks with urea as nitrogen source. This study provides a proof of principle that Ogataea polymorpha is a suitable host organism for the production of lactate using methanol as carbon source. In addition, it offers guidance for the engineering of methylotrophic organisms that produce platform chemicals from CO2-derived substrates. With reduced land use, this technology will promote the development of a sustainable industrial biotechnology in the future.

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L-lactic Acid Production from Cassava Starch by Thermotolerant Rhizopus microsporus LTH23

Cited by 6 publications

References 15 publications

Bioconversion of waste cooking oil glycerol from cabbage extract to lactic acid by Rhizopus microsporus

Bioconversion of waste cooking oil glycerol from cabbage extract to lactic acid by Rhizopus microsporus

Direct fermentation of potato starch and potato residues to lactic acid by Geobacillus stearothermophilus under non‐sterile conditions

Engineering the methylotrophic yeast Ogataea polymorpha for lactate production from methanol

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