L1 CAM expression in the superficial dorsal horn is derived from the dorsal root ganglion

Runyan, Stephen A.; Roy, Roland R.; Zhong, Hui; Phelps, Patricia E.

doi:10.1002/cne.20479

Cited by 20 publications

(32 citation statements)

References 61 publications

(63 reference statements)

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“…3A, B). These results confirm previous studies in rats in which peptidergic afferents that largely coincide with L1-positive axons in laminae I-IIo originate primarily from the small-diameter DRG nociceptors (Chung et al, 1988;Traub et al, 1989;Runyan et al, 2005). Three months after deafferentation, substantially more CGRP immunoreactive axons are present in the superficial dorsal horn at the center of the denervated region than observed in acutely deafferented wild-type mice (Fig.…”

supporting

confidence: 91%

“…1C-H). Similar to findings in adult rats (Runyan et al, 2005), L1 expression does not completely colocalize with either the peptidergic (CGRP) or nonpeptidergic (lectin IB4) axons in mice. L1-and CGRP-positive axons are double-labeled in laminae I-IIo (Fig.…”

Section: Distribution Of Small-diameter Afferents In the Dorsal Horn supporting

confidence: 76%

“…The expression patterns of L1, CGRP, IB4, and P2X 3 immunoreactivity in the superficial dorsal horn of adult wild-type mice resemble those reported in adult rats ( Fig. 1; Wiesenfeld-Hallin et al, 1984;Silverman and Kruger, 1990;Vulchanova et al, 1998;Runyan et al, 2005;Jakeman et al, 2006). L1 is concentrated in Lissauer's Tract, laminae I-II, and the dorsolateral funiculus (Figs.…”

supporting

confidence: 69%

“…Previously we identified a population of L1-positive primary afferents that sprout into the center of the denervated area following a dorsal rhizotomy in rats (Runyan et al, 2005). Here we ask if L1 CAM is required for sprouting of these CGRP/L1-positive primary afferents by examining the effects of unilateral dorsal rhizotomy at the center of the deafferented region (T12-T13) in mice lacking the L1 gene.…”

Section: Do Peptidergic Primary Afferents Sprout In L1 Mutant Mice?mentioning

confidence: 97%

“…The unilateral dorsal rhizotomy model, in which multiple dorsal roots are transected, established that intact axons from dorsal roots rostral and caudal to the rhizotomy can sprout into the partially denervated dorsal horn (Liu and Chambers, 1958;Goldberger and Murray, 1974;McNeill and Hulsebosch, 1987). Recently we used a unilateral rhizotomy model to demonstrate that L1 expression in laminae I-II is predominantly derived from the DRG and is expressed on sprouting axons that largely correlate with CGRP-labeled afferents (Runyan et al, 2005). To address the complex and sometimes contradictory role of L1 following injury we use a unilateral dorsal rhizotomy paradigm to determine if L1 is required for DRG axons to sprout by comparing the expression of peptidergic and nonpeptidergic primary afferent markers in both wild-type and L1 mutant mice.…”

Section: Nih Public Accessmentioning

confidence: 99%

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L1 cell adhesion molecule is not required for small-diameter primary afferent sprouting after deafferentation

Runyan

Roy²,

Zhong³

et al. 2007

Neuroscience

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L1 is a cell adhesion molecule associated with axonal outgrowth and fasciculation during spinal cord development and may reiterate its developmental role in adults following injury; L1 is upregulated on certain sprouting and regenerating axons in adults, but it is unclear if L1 expression is necessary for, or contributes to, regrowth of axons. This study asks if L1 is required for small-diameter primary afferents to sprout by conducting unilateral dorsal rhizotomies (6 segments; T10-L2) on both wildtype and L1 mutant mice. First we determined that L1 co-localizes substantially with the peptidergic (calcitonin gene-related peptide; CGRP) but minimally with the nonpeptidergic (isolectin B4; IB4) primary afferents in intact wild-type and L1 mutant mice. However, we encountered a complication using IB4 to identify primary afferents post-rhizotomy; we detected extensive abnormal IB4 expression in the dorsal horn and dorsal columns. Much of this aberrant IB4 labeling is associated with fibrous astrocytes and microglia. Five days after dorsal rhizotomy a large decrease in peptidergic and nonpeptidergic afferents is evident on the deafferented side in both wild-type and L1 mutants. Three months after surgery the peptidergic primary afferents sprouted into the center of the denervated dorsal horn in both wild-type and mutant mice, and quantitative analyses confirmed a sprouting density of similar magnitude in both genotypes. In contrast, we did not detect sprouting in the nonpeptidergic primary afferents in either genotype. These results suggest that the absence of L1 neither diminishes nor enhances sprouting of peptidergic small-diameter primary afferent axons following a dorsal rhizotomy. Keywordscell adhesion molecule; denervated; IB4; CGRP; nociceptors; rhizotomy The cell adhesion molecule L1 (L1 CAM) is an axonal glycoprotein and a member of the immunoglobulin superfamily (Kamiguchi et al., 1997). L1 plays a role in axonal outgrowth, fasciculation, and guidance during spinal cord development (Stallcup et al., 1985;Jessell, 1988;Stoeckli and Landmesser, 1995;Orlino et al., 2000;Tran and Phelps, 2000;Akopians et al., 2003) through homophilic and various heterophilic binding partners such as integrins (αVβ3 and α5β1) and the fibroblast growth factor receptor (Kamiguchi and Lemmon, 1997 Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Lemmon, 1997;Castellani et al., 2000). NIH Public AccessMutations in X-linked L1 in humans cause major developmental errors and result in a group of symptoms that include corpus callosum hypoplasia, spastic paraplegia and hydrocephalus (Fran...

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supporting

confidence: 91%

Section: Distribution Of Small-diameter Afferents In the Dorsal Horn supporting

confidence: 76%

supporting

confidence: 69%

Section: Do Peptidergic Primary Afferents Sprout In L1 Mutant Mice?mentioning

confidence: 97%

Section: Nih Public Accessmentioning

confidence: 99%

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L1 cell adhesion molecule is not required for small-diameter primary afferent sprouting after deafferentation

Runyan

Roy²,

Zhong³

et al. 2007

Neuroscience

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Olfactory ensheathing glia express aquaporin 1

Shields

Moore

Phelps

et al. 2010

J of Comparative Neurology

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Olfactory ensheathing glia (OEG) are distinct from other glia in their developmental origin, presence in both the peripheral and central nervous systems, and highly restricted location. OEG are present only in the olfactory lamina propria, olfactory nerve, and the outer two layers of the olfactory bulb, where they envelop bundles of olfactory sensory neuron axons, in a manner distinct from myelination. Because of their unique properties and their association with the continually generated olfactory sensory neurons, OEG have attracted interest for their potential capacity to support axonal regeneration, for example, after spinal cord injury. However, study of the properties and function of OEG has been hampered by a paucity of neurochemical markers with which to identify and distinguish them definitively from other types of glia. Here we provide evidence through anatomical colocalization studies that OEG express the water channel aquaporin 1 (AQP1), both in vivo and in vitro. We propose that AQP1 expression represents an important distinguishing characteristic of OEG, which may impart unique function to these glia.

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Neurosphere cell differentiation to aldynoglia promoted by olfactory ensheathing cell conditioned medium

Doncel‐Pérez

Caballero-Chacón

Nieto–Sampedro

2009

Glia

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Cells from central nervous system with morphology similar to radial glia and properties intermediate between astrocytes and Schwann cells were called growth-promoting glia or aldynoglia. These cells are present in adult brain olfactory bulb, hypothalamus, hypophysis, pineal gland and in the developing brain, and spinal cord (Cameron and Rakic (1991) Glia 4:124-137; Gudiño-Cabrera and Nieto-Sampedro (2000) 30:49-63). We report now that neurosphere cells, abundantly generated from E15 rat or E13 mouse corpus striatum, differentiate to aldynoglia-like cells when plated onto an adhesive substrate, and cultured in the presence of olfactory ensheathing cell conditioned medium, supplemented with EGF and bFGF. The immunophenotype, mRNA expression (microarray and RT-PCR analysis), neurite growth-promoting and ensheathing properties of the cells derived from neurospheres were similar to those of aldynoglia. Neurosphere-derived, aldynoglia-like cells expressed, with respect to neurospheres, very increased levels of GFAP, high levels of nestin and vimentin, extracellular matrix proteins, and inhibitors of the catabolism of those extracellular matrix proteins.

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L1 CAM expression in the superficial dorsal horn is derived from the dorsal root ganglion

Cited by 20 publications

References 61 publications

L1 cell adhesion molecule is not required for small-diameter primary afferent sprouting after deafferentation

L1 cell adhesion molecule is not required for small-diameter primary afferent sprouting after deafferentation

Olfactory ensheathing glia express aquaporin 1

Neurosphere cell differentiation to aldynoglia promoted by olfactory ensheathing cell conditioned medium

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