L929 cell cytotoxicity associated with experimental and commercial dental flosses

Tua-Ngam, Peerapong; Supanitayanon, L; Dechkunakorn, Surachai; Anuwongnukroh, Niwat; Srikhirin, Toemsak; Roongrujimek, Pitchaya

doi:10.1088/1757-899x/265/1/012010

Cited by 3 publications

(2 citation statements)

References 8 publications

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“…It was observed that the protein was biocompatible with a viability value of 70% or higher at all concentrations, considering that the bioavailability threshold for cells is reported to be 70%. [ 76,77 ] These results suggest that rGp144 did not induce any cytotoxic effect, indicating its potential use in biomedical and pharmaceutical applications. Moreover, evaluating the biocompatibility of RBPs for the first time in the literature is crucial for their safe usage in humans for the diagnosis and imaging of the bacterial infections.…”

Section: Resultsmentioning

confidence: 84%

The evaluation of biotechnological potential of Gp144, the key molecule of natural predator bacteriophage K in Staphylococcus aureus hunting mechanism

Tasdurmazli,

Dokuz,

Erdogdu

et al. 2023

Biotechnology Journal

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Bacteriophages, which selectively infect bacteria, and phage‐derived structures are considered promising agents for the diagnosis and treatment of bacterial infections due to the increasing antibiotic resistance. The binding of phages to their specific receptors on host bacteria is highly specific and irreversible, and therefore, the characterization of receptor‐binding proteins(RBPs), which are key determinants of phage specificity, is crucial for the development of new diagnostic and therapeutic products. This study highlights the biotechnological potential of Gp144, an RBP located in the tail baseplate of bacteriophage K and responsible for adsorption of phageK to S. aureus. Once it was established that recombinant Gp144 (rGp144)is biocompatible and does not exhibit lytic effects on bacteria, its interaction with the host, the binding efficiency and performance were assessed in vitro using microscopic and serological methods. Results showed that rGp144 has a capture efficiency (CE) of over 87% and the best CE score is %96 which captured 9 CFU mL−1 out of 10 CFU mL−1 bacteria, indicating that very low number of bacteria could be detected. Additionally, it was shown for the first time in the literature that rGp144 binds to both S. aureus and methicillin‐resistant S. aureus (MRSA) cells in vitro, while its affinity to different Gram‐positive bacteria (E. faecalis and B. cereus) was not observed. The findings suggest that rGp144 can be effectively used for the diagnosis of S. aureus and MRSA, and that the use of RBPs in host‐phage interaction can be a novel and effective strategy for imaging and diagnosing the site of infection.

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Section: Resultsmentioning

confidence: 84%

The evaluation of biotechnological potential of Gp144, the key molecule of natural predator bacteriophage K in Staphylococcus aureus hunting mechanism

Tasdurmazli,

Dokuz,

Erdogdu

et al. 2023

Biotechnology Journal

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“…This cell line was bought from Pune's National Centre for Cell Science. This assay was appropriately conducted in accordance with ISO 10993:5, a standard for evaluating the cytotoxic cellular response to medical devices and biomaterials with certain biological activities [36]. In this assay, the cells were incubated in modified eagle's medium containing fetal bovine serum at 5% CO 2 .…”

Section: In-vitro Cytotoxicity Testmentioning

confidence: 99%

Calcium silicate biocomposites: effects of selenium oxide on the physico-mechanical features and their in-vitro biological assessments

et al. 2023

Biomed. Mater.

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Bone tissue regenerative material serves as a prospective recovery candidate with self-adaptable biological properties of bio-activation, degradability, compatibility, and antimicrobial efficacy instead of metallic implants. Such materials are highly expensive due to chemical reagents and complex synthesis procedures, making them unaffordable for patients with financial constraints. This research produced an efficient bone tissue regenerative material using inexpensive naturally occurring source materials, including silica sand and limestone. The extracted SiO2 and CaO particles (75:25 wt%) were subjected to hydrothermal synthesis (water treatment instead of chemical solvents) to produce the CaSiO3 biomaterial (code: S). Selenium oxide was doped with calcium silicate at 3, 5, and 10 wt.% to enhance its properties, yielding biocomposite materials (i.e., S3, S5, and S10). The physico-mechanical properties of these materials were investigated with XRD, FTIR, FESEM-EDS, and micro-UTM. The results revealed that the synthesized biocomposites have a crystalline wollastonite phase with a porously fused rough surface. From structural parametric calculations, we found that the biocomposites have reduced particle size and enhanced surface area due to the influence of selenium oxide. The biocomposite S10, having high SeO2 content, attained the maximum compressive strength of 75.2 MPa. In-vitro studies of bioactivity, biodegradability, biocompatibility, and antibacterial activity were performed. At 7 and 14 days of bioactivity, the synthesized biocomposites are capable of dissolving their ions into SBF solution to precipitate HAp and a required Ca/P ratio of 1.69 was achieved by S3. A comparative analysis has been performed on the degradation activity in Tris-HCl and the consequent pH changes during SBF treatment. The bio-analysis revealed that the biocomposite S3 shows enhanced bioactivity through a controlled degradation rate and secured cell viability of 88% at a concentration of 100 μg/ml. It also offers significant bacterial inhibition potency against E.coli and S.aureus bacteria.

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Bone formation with high bacterial inhibition and low toxicity behavior by melding of Al2O3 on nanobioactive glass ceramics via sol-gel process

Mubina

Shailajha

Sankaranarayanan

et al. 2022

J Sol-Gel Sci Technol

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L929 cell cytotoxicity associated with experimental and commercial dental flosses

Cited by 3 publications

References 8 publications

The evaluation of biotechnological potential of Gp144, the key molecule of natural predator bacteriophage K in Staphylococcus aureus hunting mechanism

The evaluation of biotechnological potential of Gp144, the key molecule of natural predator bacteriophage K in Staphylococcus aureus hunting mechanism

Calcium silicate biocomposites: effects of selenium oxide on the physico-mechanical features and their in-vitro biological assessments

Bone formation with high bacterial inhibition and low toxicity behavior by melding of Al2O3 on nanobioactive glass ceramics via sol-gel process

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