La microglie : des cellules immunitaires qui sculptent et contrôlent les synapses neuronales

Audinat, Etienne; Arnoux, Isabelle

doi:10.1051/medsci/20143002012

Cited by 9 publications

(5 citation statements)

References 41 publications

(46 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…EtOH acts on microglial cells (Erickson et al, 2019) which are characterized by numerous extensions allowing them to analyze their microenvironment and to react accordingly (Nimmerjahn et al, 2005; Ohsawa & Kohsaka, 2011). Microglial reactivity induced by threat detection is identifiable by morphological changes such as thickening of proximal extensions and reduction in distal ramifications (Audinat & Arnoux, 2014). These changes are observable through the expression of Iba1 (ionized calcium‐binding adaptor molecule 1), a protein restricted to microglia and macrophages (Crews et al, 2016; Ito et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

Astrogliosis and compensatory neurogenesis after the first ethanol binge drinking‐like exposure in the adolescent rat

Vilpoux

Fouquet

Deschamps

et al. 2021

Alcoholism Clin & Exp Res

View full text Add to dashboard Cite

Background Multiple ethanol binge drinking‐like exposures during adolescence in the rat induce neuroinflammation, loss of neurogenesis, and cognitive deficits in adulthood. Interestingly, the first ethanol binge drinking‐like exposure during adolescence also induces short‐ term impairments in cognition and synaptic plasticity in the hippocampus though the cellular mechanisms of these effects are unclear. Here, we sought to determine which of the cellular effects of ethanol might play a role in the disturbances in cognition and synaptic plasticity observed in the adolescent male rat after two binge‐like ethanol exposures. Methods Using immunochemistry, we measured neurogenesis, neuronal loss, astrogliosis, neuroinflammation, and synaptogenesis in the hippocampus of adolescent rats 48 h after two binge‐like ethanol exposures (3 g/kg, i.p., 9 h apart). We used flow cytometry to analyze activated microglia and identify the TLR4‐expressing cell types. Results We detected increased hippocampal doublecortin immunoreactivity in the subgranular zone (SGZ) of the dentate gyrus (DG), astrogliosis in the SGZ, and a reduced number of mature neurons in the DG and in CA3, suggesting compensatory neurogenesis. Synaptic density decreased in the stratum oriens of CA1 revealing structural plasticity. There was no change in microglial TLR4 expression or in the number of activated microglia, suggesting a lack of neuroinflammatory processes, although neuronal TLR4 was decreased in CA1 and DG. Conclusions Our findings demonstrate that the cognitive deficits associated with hippocampal synaptic plasticity alterations that we previously characterized 48 h after the first binge‐like ethanol exposures are associated with hippocampal structural plasticity, astrogliosis, and decreased neuronal TLR4 expression, but not with microglia reactivity.

show abstract

Section: Discussionmentioning

confidence: 99%

Astrogliosis and compensatory neurogenesis after the first ethanol binge drinking‐like exposure in the adolescent rat

Vilpoux

Fouquet

Deschamps

et al. 2021

Alcoholism Clin & Exp Res

View full text Add to dashboard Cite

show abstract

“…La microglie constitue une population de cellules gliales à activité phagocytaire, connue comme formant la principale défense immunitaire du système nerveux central. Les astrocytes, quant à eux, jouent un rôle important dans le maintien et la protection des neurones, mais aussi dans la défense immunitaire et la réparation du système nerveux central après lésion [6] (➜). Le rôle de ces deux types cellulaires dans le maintien de l'intégrité du système nerveux central est intéressant à considérer au regard de leur faible permissivité à l'infection.…”

Section: Discussionunclassified

Cultures 3D de cerveau

Froment

Erraiss

2021

Med Sci (Paris)

View full text Add to dashboard Cite

Le dossier que nous présentons a été rédigé par les étudiantes et étudiants de Master 1 de Biologie de l’École normale supérieure de Lyon à l’issue de l’UE Microbiologie moléculaire et structurale (2020-2021). Le Master de biologie de l’ENS de Lyon accueille chaque année environ 40 étudiants en M1 et en M2 et propose une formation de haut niveau à la recherche en biosciences. Chaque étudiant y construit son parcours à la carte, en choisissant ses options parmi un large panel de modules, favorisant ainsi une approche pluridisciplinaire des sciences du vivant, et cela en relation étroite avec les laboratoires de recherche du tissu local, national et international. En participant à diverses activités scientifiques liées aux UE de leur formation, les étudiants préparent également l’obtention du Diplôme de l’ENS de Lyon, qui valide leur scolarité à l’ENS. La rédaction du présent dossier, qui vise à transmettre de façon claire les messages issus d’une sélection d’articles scientifiques publiés récemment dans le domaine de la microbiologie, constitue l’une de ces activités connexes proposées aux étudiants.

show abstract

“…Activation of P2X7R has been shown to induce microglial migration to the sites of injury and phagocytosis [ 12 , 70 ]. Consistent with these reports, our studies show that P2X7R is an important regulator of microglial effector function in response to stimulant abuse.…”

Section: Discussionmentioning

confidence: 99%

Methamphetamine alters microglial immune function through P2X7R signaling

Fernandes

Sriram

Gofman

et al. 2016

J Neuroinflammation

View full text Add to dashboard Cite

Background: Purinoceptors have emerged as mediators of chronic inflammation and neurodegenerative processes. The ionotropic purinoceptor P2X7 (P2X7R) is known to modulate proinflammatory signaling and integrate neuronal-glial circuits. Evidence of P2X7R involvement in neurodegeneration, chronic pain, and chronic inflammation suggests that purinergic signaling plays a major role in microglial activation during neuroinflammation. In this study, we investigated the effects of methamphetamine (METH) on microglial P2X7R.

show abstract

La microglie : des cellules immunitaires qui sculptent et contrôlent les synapses neuronales

Cited by 9 publications

References 41 publications

Astrogliosis and compensatory neurogenesis after the first ethanol binge drinking‐like exposure in the adolescent rat

Astrogliosis and compensatory neurogenesis after the first ethanol binge drinking‐like exposure in the adolescent rat

Cultures 3D de cerveau

Methamphetamine alters microglial immune function through P2X7R signaling

Contact Info

Product

Resources

About