2007
DOI: 10.1002/elan.200704001
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Lab‐on‐a‐Chip Biosensor for Glucose Based on a Packed Immobilized Enzyme Reactor

Abstract: In this work, the development of a packed immobilized enzyme reactor (IMER) and its integration to a capillary electrophoresis microchip is described. The present microchip design differs from others, in the fact that the same design could be used with or without the particles and, just by changing the material used to pack the IMER, different analytes can be detected. The applied procedure involves the separation of the target analyte by capillary electrophoresis (CE), which is then coupled to a post-column I… Show more

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Cited by 22 publications
(14 citation statements)
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“…Additions of fructose showed no effect on either the peak position or the peak magnitude of glucose. The microchip-CE-IMER was used to quantify glucose in carbonated beverages with a good agreement to other reports (Blanes et al 2007). An extensive summary of glucose biosensor constructions and their applications can be found in a review written by Wang (Wang 2008).…”
Section: Glucosementioning
confidence: 93%
“…Additions of fructose showed no effect on either the peak position or the peak magnitude of glucose. The microchip-CE-IMER was used to quantify glucose in carbonated beverages with a good agreement to other reports (Blanes et al 2007). An extensive summary of glucose biosensor constructions and their applications can be found in a review written by Wang (Wang 2008).…”
Section: Glucosementioning
confidence: 93%
“…As a compromise between signal magnitude and selectivity,[45, 46] the oxidation of hydrogen peroxide was performed at +650 mV (vs. Ag|AgCl|KCl sat ) and followed by chronoamperometry.…”
Section: Methodsmentioning
confidence: 99%
“…One possibility is the definition of microfluidic structures including steps (Ito et al, 2011;Malecha et al, 2009) or micropillars (Jang and Koh, 2010;Kim et al, 2009) which stop the movement of the particles in the fluid stream at one point of the system. Wires incorporated into microfluidic channels can serve the same purpose (Blanes et al, 2007). The other typical method to retain microparticles is the usage of magnetic particles (Hernandez et al, 2013;Lin et al, 2013;Sheng et al, 2012).…”
Section: Fabricationmentioning
confidence: 98%