Lab-on-a-Chip Systems for Cellular Assays

Wolf, Bernhard; Brischwein, M.; Grothe, H.; Stepper, Christoph; Ressler, J.; Weyh, Thomas

doi:10.1007/978-0-387-28732-4_9

Cited by 9 publications

(3 citation statements)

References 104 publications

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“…In such a way a bacterial growth sensor could be accomplished and growth inhibition tests on a microscale with accelerated measuring times were established [98]. A comprehensive overview of cell-based monitoring devices is given in the literature [99].…”

Section: Biosensor Arrays For Monitoring Cell-based Metabolic Parametersmentioning

confidence: 99%

Micro- and nanobiosensors—state of the art and trends

Urban

2008

Meas. Sci. Technol.

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Recent progress in microsystem technologies for creating small, integrated and reliable microelectronic devices and microtransducers in combination with biological sensing elements has raised the expectation to get a comprehensive insight into dynamic cellular metabolic events and subsequently a complete understanding of the metabolism of human biology. Such micro- and nanobiosensor tools in combination with appropriate microfluidics enable the simultaneous description and moreover monitoring of gene, protein expression and metabolic states in a biosystem. This knowledge will lead to new future therapies and pharmaceutical treatments. Furthermore, with such methods and microdevices a new direct combination of diagnostics and immediately starting therapy can be established which is called theranostic. There are a lot of challenging problems to be overcome to reach this goal: microsystems monitoring of physical parameters in biological systems is well established, whereas chemo- and biosensors are available for only a few parameters and mainly as in vitro devices. Gene arrays entered the research market but are up to now not in clinical practice. Protein arrays are in a developmental state with promising results, but far from a mature reproducible state. For getting insights into metabolic events intra- and extracellularly, some new and promising technologies are under research and will undoubtedly revolutionize systems biology but only in the longer run. As a conclusion, it can be stated that new methods stemming from microelectronics and biological technologies are now entering the world of chemical and biological analytics as well as clinics to offer clinicians new theranostic tools in the future.

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Section: Biosensor Arrays For Monitoring Cell-based Metabolic Parametersmentioning

confidence: 99%

Micro- and nanobiosensors—state of the art and trends

Urban

2008

Meas. Sci. Technol.

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“…While micro total analysis systems (µTAS) [Daridon et al 2001, Kovarik et al 2012 were originally developed for chemical analysis, lab-on-chip systems (LOCs) [Becker et al 1995, Dürr et al 2003, El-Ali et al 2006, Fuhr et al 1997, Goater et al 1997, Wolf et al 2006 were developed to monitor physiological cell parameters, cell manipulation and sorting, or for use as micro-bioreactors. Nowadays, a distinction between the two types of microsystems seems somewhat obsolete.…”

Section: Introductionmentioning

confidence: 99%

A short tutorial contribution to impedance and AC-electrokinetic characterization and manipulation of cells and media: Are electric methods more versatile than acoustic and laser methods?

Gimsa

Stubbe

Gimsa

2014

Journal of Electrical Bioimpedance

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Lab-on-chip systems (LOCs) can be used as in vitro systems for cell culture or manipulation in order to analyze or monitor physiological cell parameters. LOCs may combine microfluidic structures with integrated elements such as piezo-transducers, optical tweezers or electrodes for AC-electrokinetic cell and media manipulations. The wide frequency band (<1 kHz to >1 GHz) usable for AC-electrokinetic manipulation and characterization permits avoiding electrochemical electrode processes, undesired cell damage, and provides a choice between different polarization effects that permit a high electric contrast between the cells and the external medium as well as the differentiation between cellular subpopulations according to a variety of parameters. It has been shown that structural polarization effects do not only determine the impedance of cell suspensions and the force effects in AC-electrokinetics but can also be used for the manipulation of media with inhomogeneous temperature distributions. This manuscript considers the interrelations of the impedance of suspensions of cells and AC-electrokinetic single cell effects, such as electroorientation, electrodeformation, dielectrophoresis, electrorotation, and travelling wave (TW) dielectrophoresis. Unified models have allowed us to derive new characteristic equations for the impedance of a suspension of spherical cells, TW dielectrophoresis, and TW pumping. A critical review of the working principles of electro-osmotic, TW and electrothermal micropumps shows the superiority of the electrothermal pumps. Finally, examples are shown for LOC elements that can be produced as metallic structures on glass chips, which may form the bottom plate for self-sealing microfluidic systems. The structures can be used for cell characterization and manipulation but also to realize micropumps or sensors for pH, metabolites, cell-adhesion, etc.

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“…Previous methods often neglecting parallel, dynamic and non-linear signaling patterns of cells-requiring individual testswere unable to do this. 6,7 Figure 1 shows the basic setup of such a test system, indicating how the signal parameters of the cells couple into the chip. This fundamental principle applies for further developments of the system and permits online and real-time analysis of cellular signal behavior.…”

Section: Introductionmentioning

confidence: 99%

Electronics for better healthcare

Wolf

Herzog

2013

Eur J Prev Cardiolog

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Microelectronics and microsystem technology have changed our daily lives considerably in the past 50 years. Countless everyday objects contain microelectronic components. In healthcare up to the present, however, it has not been possible to make major alterations in introducing electronics and information technology that would lead to innovative improvements and greater transparency. This paper describes initial steps in diagnostics and oncological therapy including telematic healthcare systems which can, for example, assist patients with cardiovascular diseases and shows, through these areas, how electronics and microsystems technology can contribute to better healthcare.

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Lab-on-a-Chip Systems for Cellular Assays

Cited by 9 publications

References 104 publications

Micro- and nanobiosensors—state of the art and trends

Micro- and nanobiosensors—state of the art and trends

A short tutorial contribution to impedance and AC-electrokinetic characterization and manipulation of cells and media: Are electric methods more versatile than acoustic and laser methods?

Electronics for better healthcare

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