Lab Pearls: Making Great Slides

Lutz, Alex

doi:10.1017/cbo9780511581236.008

Cited by 2 publications

(3 citation statements)

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“…If this fails, he suggests taking initial thin cuts in order to achieve mono-layer cells and only then to take one or two thick cuts. 14 As seen in Fig. 3a,b, the fourth and fifth discordant cases both displayed a single, small piece of tumour at the deepest edge in specimens, which were divided into halves after excision.…”

Section: Discussionmentioning

confidence: 91%

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Concordance between a Mohs surgeon and a dermatopathologist in evaluating Mohs cryosections

Atilla

Gököz

Özer

et al. 2021

Acad Dermatol Venereol

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Background Mohs micrographic surgery is the gold standard treatment for high-risk non-melanoma skin cancers. The success of Mohs relies on accurate histopathologic evaluation. Due to law restrictions in some European countries, Mohs surgeons are not permitted to report on histopathology; therefore, a pathologist evaluates the frozen sections. Objective To retrospectively assess the concordance between the certified Mohs surgeon and the pathologist in evaluating the Mohs slides that were intraoperatively evaluated by the pathologist. Materials and MethodsFrozen section slides of a total of 237 Mohs cases between 2013 and 2020 were examined by the blinded Mohs surgeon, and the tumours were marked on copy maps. The copy maps and the original maps were compared, and the non-concordant cases were re-evaluated together by the Mohs surgeon and the dermatopathologist.The concordance rate was calculated, and the inter-rater agreement was statistically analysed using Cohen's Kappa coefficient. ResultsWe report a high concordance rate (97.9%) and inter-rater agreement (0.96) between Mohs surgeon and dermatopathologist in evaluating Mohs slides. ConclusionAs a newly settled centre, our results are in alignment with experienced centres where the Mohs surgeon evaluates the slides herself and performs the surgery.

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Section: Discussionmentioning

confidence: 91%

“…Instead of taking thick sections, Lutz suggested freezing adipose tissue with liquid nitrogen to help fatty sections. If this fails, he suggests taking initial thin cuts in order to achieve mono‐layer cells and only then to take one or two thick cuts 14 …”

Section: Discussionmentioning

confidence: 99%

Concordance between a Mohs surgeon and a dermatopathologist in evaluating Mohs cryosections

Atilla

Gököz

Özer

et al. 2021

Acad Dermatol Venereol

View full text Add to dashboard Cite

show abstract

“…Such approaches include retaining malleability by keeping cartilage moist, using positively charged slides to improve adherence, gentle heating, and acetone fixation. 1,2 Previously reported methods that target curvatures include relaxing incisions and positioning samples using buttresses created from embedding medium. 1,3,4 A case involving serpentine complex contours of the ear is presented, and it highlights grossing strategies to separate each curve into a distinct piece.…”

mentioning

confidence: 99%