Label-free and portable field-effect sensor for monitoring RT-LAMP products to detect SARS-CoV-2 in wastewater

Alvarez-Serna, Bryan E.; Ramírez-Chavarría, Roberto G.; Castillo-Villanueva, Elizabeth; Carrillo‐Reyes, Julián; Ramírez-Zamora, Rosa-María; Buitrón, Germán; Álvarez-Icaza, Luis

doi:10.1016/j.talanta.2022.124060

Cited by 11 publications

(5 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Integrating a reverse transcription loop-mediated isothermal amplification (RT-LAMP) reaction as a low-cost molecular test with high specificity, this sensor offers a novel method for identifying the N and ORF1ab genes within the SARS-CoV-2 genome. The detection limit for the SARS-CoV-2 genome in actual wastewater samples was 0.31 × 10 −3 ng/µL [47]. As science and technology continue to advance, more efficient and accurate automated detection techniques are anticipated to emerge, catering to the demands of sewage epidemiological research.…”

Section: Emerging Analysis and Detection Techniques For Biomarkersmentioning

confidence: 99%

Research Progress and Perspectives on Wastewater-Based Epidemiology: A Bibliometric Analysis

Yang,

Jin,

Song

et al. 2024

Water

View full text Add to dashboard Cite

Wastewater-based epidemiology (WBE) evaluates the health status, environmental exposure, and lifestyle habits of community inhabitants through the investigation of chemical or biological markers present in urban wastewater systems. This approach is frequently employed in discerning drug abuse, disease prevalence, and the presence of environmental contaminants. To comprehend the current state and developmental trajectories in WBE research, the current study utilizes the source literature of the Web of Science Core Collection (WOSCC) database. Implementing the Bibliometrix toolkit in R language and employing CiteSpace and VOSviewer for bibliometric analysis, this investigative pursuit effectuates an all-encompassing evaluation of the WBE literature, traversing a substantial time span of 16 years, encompassing 2008 through 2023. The results of this bibliometric analysis illuminate annual propensities and disciplinary distribution related to WBE research, while discerning the most impactful and prolific contributors, including authors, institutions, countries, and scholarly journals. The onset of the COVID-19 pandemic has engendered the expedited progression of WBE, leading to a substantial escalation in research endeavors in the past three years. By meticulously evaluating highly-cited publications, co-occurrence network of keywords, and keyword burst analysis, it is concluded that the research hotspots in this field focus on the monitoring of illicit drugs, psychoactive substances, and viruses in sewage. Subsequent investigations possess the capacity to propel the advancement of emerging methodologies for biomarker identification and analytical techniques. By concurrently integrating big data technologies (including artificial intelligence and cloud computing) with epidemiological and clinical data sets, a more expansive, precise, and efficacious rendition of WBE research can be realized.

show abstract

Section: Emerging Analysis and Detection Techniques For Biomarkersmentioning

confidence: 99%

Research Progress and Perspectives on Wastewater-Based Epidemiology: A Bibliometric Analysis

Yang,

Jin,

Song

et al. 2024

Water

View full text Add to dashboard Cite

show abstract

“…The specificity of detection is determined by the ability of LAMP to recognize the target sequence. [ 178,179 ] The nonimmobilization method in transistor‐based biosensors simplifies the biofunctionalization process and reduces costs, providing label‐free, real‐time monitoring of biochemical reactions, which promises a universal platform for biosensing.…”

Section: Functionalization Of Transistors For Biosensorsmentioning

confidence: 99%

“…The specificity of detection is determined by the ability of LAMP to recognize the target sequence. [178,179] The nonimmobilization method in transistor-based biosensors simplifies the biofunctionalization process and reduces costs, providing label-free, real-time monitoring of biochemical reactions, which promises a universal platform for biosensing. [98] Copyright 2021, The Authors, published by The Hong Kong Polytechnic University and John Wiley & Sons Australia, Ltd. b) A diagram of the OFET-based biosensor designed for procalcitonin (PCT) detection.…”

Section: Nonimmobilized Secondary Sensing Strategymentioning

confidence: 99%

Recent Advances in Field‐Effect Transistor‐Based Biosensors for Label‐Free Detection of SARS‐CoV‐2

Yu,

Zhang,

Liu

et al. 2023

Small Science

View full text Add to dashboard Cite

Field‐effect transistor‐based biosensors (FET biosensors) have gained significant attention in the context of the global SARS‐CoV‐2 pandemic owing to their label free and highly sensitive detection capabilities. In this comprehensive review, recent advances in the use of electrochemical‐transistor‐based biosensors for the label‐free detection of SARS‐CoV‐2 are analyzed. A general introduction to the electrochemical‐transistor‐based biosensor system is initiated, highlighting its critical technical requirements. Various structural designs and working principles of transistor‐based transducers are described, and the essential aspects of interface engineering for improved sensing performance are summarized. The applications of transistor‐based biosensors for the detection of SARS‐CoV‐2 are discussed in detail. Finally, perspectives for the future development of transistor‐based sensing systems are provided. This review aims to provide valuable insights and guidance for the design and optimization of biochemical sensor systems with the potential to impact health monitoring, disease diagnosis, and biosafety in the field of in vitro diagnostic products.

show abstract

“…Furthermore, since the COVID-19 pandemic, the use of isothermal reactions coupled with automated and portable sensing, pave the road to develop innovative analytical devices. For instance, for emergent pathogens one can find recent developments based on optical detection [9], electrochemical transduction [10], nanomaterial-based sensors [11], colorimetric assays [12] and field-effect devices [13], to mention only a few. Jointly, there is a current need to provide solutions not only for viral particles, but also for easy-to-use and rapid molecular tests.…”

Section: Introductionmentioning

confidence: 99%

Automatic Analysis of Isothermal Amplification via Impedance Time-Constant-Domain Spectroscopy: A SARS-CoV-2 Case Study

et al. 2023

Self Cite

View full text Add to dashboard Cite

The development of sensitive and affordable testing devices for infectious diseases is essential to preserve public health, especially in pandemic scenarios. In this work, we have developed an attractive analytical method to monitor products of genetic amplification, particularly the loop-mediated isothermal amplification reaction (RT-LAMP). The method is based on electrochemical impedance measurements and the distribution of relaxation times model, to provide the so-called time-constant-domain spectroscopy (TCDS). The proposed method is tested for the SARS-CoV-2 genome, since it has been of worldwide interest due to the COVID-19 pandemic. Particularly, once the method is calibrated, its performance is demonstrated using real wastewater samples. Moreover, we propose a simple classification algorithm based on TCDS data to discriminate among positive and negative samples. Results show how a TCDS-based method provides an alternative mechanism for label-free and automated assays, exhibiting robustness and specificity for genetic detection.

show abstract

Label-free and portable field-effect sensor for monitoring RT-LAMP products to detect SARS-CoV-2 in wastewater

Cited by 11 publications

References 47 publications

Research Progress and Perspectives on Wastewater-Based Epidemiology: A Bibliometric Analysis

Research Progress and Perspectives on Wastewater-Based Epidemiology: A Bibliometric Analysis

Recent Advances in Field‐Effect Transistor‐Based Biosensors for Label‐Free Detection of SARS‐CoV‐2

Automatic Analysis of Isothermal Amplification via Impedance Time-Constant-Domain Spectroscopy: A SARS-CoV-2 Case Study

Contact Info

Product

Resources

About