Label-free and reagentless capacitive aptasensor for thrombin

Chen, Hsin-Ju; Chen, Richie L.C.; Hsieh, Bo-Chuan; Hsiao, Hsien-Yi; Kung, Yi; Hou, Yung-Te; Cheng, Tzong‐Jih

doi:10.1016/j.bios.2019.02.025

Cited by 40 publications

(18 citation statements)

References 53 publications

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“…[Fe(CN)6]-3/-4 was applied as a redox indicator. The proposed aptasensor showed a response toward thrombin in the range from 10 pM to 1 µM (0.36 µg•L −1 to 0.036 g•L −1 ) with a lower limit of detection of 10 pM (0.36 µg•L −1 ) [144].…”

Section: Coagulation Biomarkers Thrombinmentioning

confidence: 99%

From Small Molecules toward Whole Cells Detection: Application of Electrochemical Aptasensors in Modern Medical Diagnostics

Ziółkowski

Jarczewska

Górski

et al. 2021

Sensors

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This paper focuses on the current state of art as well as on future trends in electrochemical aptasensors application in medical diagnostics. The origin of aptamers is presented along with the description of the process known as SELEX. This is followed by the description of the broad spectrum of aptamer-based sensors for the electrochemical detection of various diagnostically relevant analytes, including metal cations, abused drugs, neurotransmitters, cancer, cardiac and coagulation biomarkers, circulating tumor cells, and viruses. We described also possible future perspectives of aptasensors development. This concerns (i) the approaches to lowering the detection limit and improvement of the electrochemical aptasensors selectivity by application of the hybrid aptamer–antibody receptor layers and/or nanomaterials; and (ii) electrochemical aptasensors integration with more advanced microfluidic devices as user-friendly medical instruments for medical diagnostic of the future.

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Section: Coagulation Biomarkers Thrombinmentioning

confidence: 99%

From Small Molecules toward Whole Cells Detection: Application of Electrochemical Aptasensors in Modern Medical Diagnostics

Ziółkowski

Jarczewska

Górski

et al. 2021

Sensors

View full text Add to dashboard Cite

show abstract

“…Thrombin is a type of serine protease essential to molecular biology for tumor growth, metastasis, angiogenesis, and blood coagulation [90]. It is used primarily as a tumor marker to diagnose pulmonary metastasis [91,92].…”

Section: Electrochemical Detection Of Thrombinmentioning

confidence: 99%

Recent Advances in Electrochemical Sensors for the Detection of Biomolecules and Whole Cells

2020

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Electrochemical sensors are considered an auspicious tool to detect biomolecules (e.g., DNA, proteins, and lipids), which are valuable sources for the early diagnosis of diseases and disorders. Advances in electrochemical sensing platforms have enabled the development of a new type of biosensor, enabling label-free, non-destructive detection of viability, function, and the genetic signature of whole cells. Numerous studies have attempted to enhance both the sensitivity and selectivity of electrochemical sensors, which are the most critical parameters for assessing sensor performance. Various nanomaterials, including metal nanoparticles, carbon nanotubes, graphene and its derivatives, and metal oxide nanoparticles, have been used to improve the electrical conductivity and electrocatalytic properties of working electrodes, increasing sensor sensitivity. Further modifications have been implemented to advance sensor platform selectivity and biocompatibility using biomaterials such as antibodies, aptamers, extracellular matrix (ECM) proteins, and peptide composites. This paper summarizes recent electrochemical sensors designed to detect target biomolecules and animal cells (cancer cells and stem cells). We hope that this review will inspire researchers to increase their efforts to accelerate biosensor progress—enabling a prosperous future in regenerative medicine and the biomedical industry.

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“…In Faradic impedance mode, a redox couple is utilized, and the reaction occur due to the charge transfer through the electron traveling to/from the electrode surface [81]. Alternatively, in the non-faradaic mode, the changing currents are dominant and capacitive changes occurring due to the charge distribution, surface dielectric, or local conductance [82]. Furthermore, the non-faradaic approaches have the advantages comparing with the faradaic, due to non-faradaic reaction does not require pre-addition of redox probes to the analytical solution [18].…”

Section: A Capacitive-based Biosensors For Chemicals and Pesticides mentioning

confidence: 99%

Recent Progress in the Development of Biosensors for Chemicals and Pesticides Detection

et al. 2020

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Chemicals and pesticides contamination in the food, drinking water, and environment ecosystem have become one of the most serious problems for human public health in the world due to their large amount used and wide application in the agriculture industry. Therefore, the detection and analysis of contamination in the food and drinking water by using techniques that are simple and suitable for fast screening are important. This review gives an overview of the last trends and recent advances biosensors for chemicals and pesticides detection based on electrochemical, optical and mechanical transducers strategies. Furthermore, the biosensors are classified according to their immobilized biorecognition elements including aptamer, antibodies, enzymes, and molecularly imprinted polymers. The implementation of nanomaterials such as graphene, carbon nanotubes, and metals nanoparticles are also emphasized and discussed in this review, these nanomaterials provides remarkable features to the biosensors such highly sensitive and accurate which allowing efficient pesticides detection. In addition to highlighting and summarizing various novel sensors, this review also provided some drawbacks, challenging, prospects as well as the current efforts to enhanced optical sensors. INDEX TERMS Biosensors, chemical detection, electrochemical transducer, mechanical transducer, optical transducer, pesticides detection.

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Label-free and reagentless capacitive aptasensor for thrombin

Cited by 40 publications

References 53 publications

From Small Molecules toward Whole Cells Detection: Application of Electrochemical Aptasensors in Modern Medical Diagnostics

From Small Molecules toward Whole Cells Detection: Application of Electrochemical Aptasensors in Modern Medical Diagnostics

Recent Advances in Electrochemical Sensors for the Detection of Biomolecules and Whole Cells

Recent Progress in the Development of Biosensors for Chemicals and Pesticides Detection

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