Label-Free Biosensors

Pasquarelli, A.

doi:10.1007/978-3-030-76469-2_6

Cited by 1 publication

(1 citation statement)

References 44 publications

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“…Label-free biosensors have emerged also as powerful analytical devices for detecting and quantifying biomolecules in various fields, e.g., environmental monitoring. They can detect a wide range of analytes, including proteins, nucleic acids, and small molecules, making them versatile tools for diverse applications (Cooper, 2009;Schöning and Poghossian, 2018;Pasquarelli, 2021;Samuel and Rao, 2022). These biosensors offer several advantages over traditional immunoassays such as the Enzyme-Linked Immunosorbent Assay (ELISA).…”

Section: Introductionmentioning

confidence: 99%

Label-free electrochemical immunosensor as a reliable point-of-care device for the detection of Interleukin-6 in serum samples from patients with psoriasis

Cancelliere,

Cosio,

Campione

et al. 2023

Front. Chem.

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Interleukin-6 (IL-6) plays a crucial role in autoimmunity and chronic inflammation. This study aims to develop a low-cost, simple-to-manufacture, and user-friendly label-free electrochemical point-of-care device for the rapid detection of IL-6 in patients with psoriasis. Precisely, a sandwich-based format immunosensor was developed using two primary antibodies (mAb-IL6 clone-5 and clone-7) and screen-printed electrodes modified with an inexpensive recycling electrochemical enhancing material, called biochar. mAb-IL6 clone-5 was used as a covalently immobilized capture bioreceptor on modified electrodes, and mAb-IL6 clone-7 was used to recognize the immunocomplex (Anti-IL6 clone-5 and IL-6) and form the sandwich. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to conduct electrochemical characterization of the layer-by-layer assembly of the immunosensor, while square wave voltammetry (SWV) was used to perform the sensing. The developed immunosensor demonstrated robust analytical performance in buffer solution, with a wide linear range (LR) by varying from 2 to 250 pg/mL, a good limit of detection (LOD) of 0.78 pg/mL and reproducibility (RSD<7%). In addition, a spectrophotometric ELISA kit was employed to validate the results obtained with the label-free device by analyzing twenty-five serum samples from control and patients affected by psoriasis. A strong correlation in terms of pg/mL concentration of IL-6 was found comparing the two methods, with the advantage for our label-free biosensor of an ease use and a quicker detection time. Based on IL-6 levels, the proposed immunosensor is a dependable, non-invasive screening device capable of predicting disease onset, progression, and treatment efficacy.

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Section: Introductionmentioning

confidence: 99%