Label-free cell profiling

Schasfoort, Richardus B.M.; Bentlage, Arthur E. H.; Stojanović, Ivan; Kooi, Alex van der; Schoot, Ellen van der; Terstappen, Leon W.M.M.; Vidarsson, Gestur

doi:10.1016/j.ab.2013.04.001

Cited by 26 publications

(39 citation statements)

References 8 publications

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“…Surface plasmon resonance (SPR) measurements analyzing protein-protein interactions were carried out in an IBIS MX96 (IBIS Technologies, Enschede, The Netherlands) as described by de Lau et al 19 Cellular SPR (cSPR) measuring the interaction of whole platelets with biosensor-coupled protein ligands was carried out on the IBIS MX96 using methods based on the principles described in Schasfoort et al 20 Both are described in details in the supplemental Methods. …”

Section: Surface Plasmon Resonancementioning

confidence: 99%

“…To investigate the binding of CRP to platelets more closely, we used a sensitive cSPR method that we recently developed for red blood cells but now applied to platelets. 20 With this method, the platelets were allowed to interact with up to 48 spots, each coupled with different ligands, and the platelet interaction to each spot was measured simultaneously in real time. Platelets (HPA-1a 1 /HPA-5b 2 ) opsonized with anti-HPA-1a…”

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confidence: 99%

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C-reactive protein enhances IgG-mediated phagocyte responses and thrombocytopenia

Kapur

Heitink‐Pollé

Porcelijn

et al. 2015

Blood

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Key Points• CRP enhances IgG-mediated respiratory burst and phagocytosis of platelets in vitro and their clearance in vivo.• CRP levels are increased in ITP patients and correlate with platelet counts and bleeding severity and predict time to recovery.Immune-mediated platelet destruction is most frequently caused by allo-or autoantibodies via Fcg receptor-dependent phagocytosis. Disease severity can be predicted neither by antibody isotype nor by titer, indicating that other factors play a role. Here we show that the acute phase protein C-reactive protein (CRP), a ligand for Fc receptors on phagocytes, enhances antibody-mediated platelet destruction by human phagocytes in vitro and in vivo in mice. Without antiplatelet antibodies, CRP was found to be inert toward platelets, but it bound to phosphorylcholine exposed after oxidation triggered by antiplatelet antibodies, thereby enhancing platelet phagocytosis. CRP levels were significantly elevated in patients with allo-and autoantibody-mediated thrombocytopenias compared with healthy controls. Within a week, intravenous immunoglobulin treatment in children with newly diagnosed immune thrombocytopenia led to significant decrease of CRP levels, increased platelet numbers, and clinically decreased bleeding severity. Furthermore, the higher the level of CRP at diagnosis, the longer it took before stable platelet counts were reached. These data suggest that CRP amplifies antibody-mediated platelet destruction and may in part explain the aggravation of thrombocytopenia on infections. Hence, targeting CRP could offer new therapeutic opportunities for these patients. (Blood. 2015;125(11): 1793-1802 IntroductionFetal or neonatal alloimmune thrombocytopenia (FNAIT) and immune thrombocytopenia (ITP) are both antibody-mediated disorders in which platelets are destroyed mainly through activating immunoglobulin (IgG) Fc receptors on phagocytes in the spleen and liver, eventually resulting in thrombocytopenia. 1 In childhood, ITP is characterized by a typical history of acute development of purpura and bruising in an otherwise healthy child, caused by development of platelet autoantibodies, with an incidence of ;5 in 100 000 children.2 Most children with newly diagnosed ITP will not suffer from serious bleeding and will recover within 12 months. In ;60% of ITP, there is a history of a prior infection. [1][2][3] FNAIT is a potentially destructive disease in pregnancies, with intracerebral hemorrhage (ICH) of the fetus or neonate as the most feared complication, resulting in perinatal death in 1% to 7% or in severe neurological impairments in 14% to 26% of affected pregnancies. [4][5][6][7] FNAIT is caused by maternal IgG platelet alloantibodies, in whites mainly directed against human platelet antigen (HPA)-1a (85%), that cross the placenta and destroy the platelets of the fetus or newborn. Immunization against HPA-1a occurs in ;1:450 random pregnancies. [8][9][10] Although platelet decrement is related to antibody titer in FNAIT, 8,11,12 this correlation is not strict, as cas...

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Section: Surface Plasmon Resonancementioning

confidence: 99%

mentioning

confidence: 99%

C-reactive protein enhances IgG-mediated phagocyte responses and thrombocytopenia

Kapur

Heitink‐Pollé

Porcelijn

et al. 2015

Blood

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“…Quantitative assessment of agglutination can help the identification of subtypes with varying antigen densities 11 . Technologies using surface coating and monitoring interactions of RBC with the functionalized surface require specialized instruments for readout, such as surface plasmon resonance 12, 13 detection. The approach we present here is based on device surface-RBC interactions, in a simple microfluidic device, yet with an easily quantifiable readout (Table 2).…”

Section: Discussionmentioning

confidence: 99%

Detection of red blood cell surface antigens by probe-triggered cell collision and flow retardation in an autonomous microfluidic system

Sautner

Papp

Holczer

et al. 2017

Sci Rep

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Microfluidic devices exploit combined physical, chemical and biological phenomena that could be unique in the sub-millimeter dimensions. The current goal of development of Point-of-Care (POC) medical devices is to extract the biomedical information from the blood. We examined the characteristics of blood flow in autonomous microfluidic devices with the aim to realize sensitive detection of interactions between particulate elements of the blood and the appropriately modified surfaces of the system. As a model experiment we demonstrated the fast analysis of the AB0 blood group system. We observed that the accumulation of red blood cells immobilized on the capillary wall leads to increased lateral movement of the flowing cells, resulting in the overall selective deceleration of the red blood cell flow column compared to the plasma fraction. We showed that by monitoring the flow rate characteristics in capillaries coated with blood type reagents it is possible to identify red blood cell types. Analysis of hydrodynamic effects governing blood flow by Finite Element Method based modelling supported our observations. Our proof-of-concept results point to a novel direction in blood analysis in autonomous microfluidic systems and also provide the basis for the construction of a simple quantitative device for blood group determination.

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“…SPR for blood group antigen detection 22, 23 and antibody detection 24–26 has been reported. Quinn et al .…”

Section: Introductionmentioning

confidence: 99%

“…More recently, another platform utilizing SPR image analysis demonstrated multiplex RBC typing 23 . This study highlighted the ability to detect the presence of A, M, and D antigens on the RBC surface using each antigen’s corresponding IgG antibody, and provided sedimentation profiles to that of the whole cells flowing through SPR imaging device.…”

Section: Introductionmentioning

confidence: 99%

Quantitative Detection of Weak D Antigen Variants in Blood Typing using SPR

Then

Aguilar

Garnier

2017

Sci Rep

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Modern techniques for quantifying blood group antibody-antigen interactions are very limited, especially for weaker interactions which result from low antigen expression and/or partial expression of the antigen structure. Surface plasmon resonance (SPR) detection is often used to monitor and quantify bio-interactions. Previously, a regenerable, multi-fucntional platform for quantitative RBC phenotyping of normal antigen expression using SPR detection was reported. However, detection of weaker variants were not explored. Here, this sensitivity study used anti-human IgG antibodies immobilized to a gold sensor surface to two clinically important types of weaker D variants using SPR; weak D and partial D. Positive pre-sensitised cells bind to the anti-human IgG monolayer, and the response unit (RU) is reported (>100 RU). Unbound negative cells are directly eluted (<100 RU). Weak D cells were detected between a range of 180–580 RU, due to a lower expression of antigens. Partial D cells, category D VI, were also positively identified (352–1147 RU), similar to that of normal D antigens. The detection of two classes of weaker D variants was achieved for the first time using this fully regenerable SPR platform, opening up a new avenue to replace the current subjective and arbitrary methods for quantifying blood group antibody-antigen interactions.

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Label-free cell profiling

Cited by 26 publications

References 8 publications

C-reactive protein enhances IgG-mediated phagocyte responses and thrombocytopenia

C-reactive protein enhances IgG-mediated phagocyte responses and thrombocytopenia

Detection of red blood cell surface antigens by probe-triggered cell collision and flow retardation in an autonomous microfluidic system

Quantitative Detection of Weak D Antigen Variants in Blood Typing using SPR

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