Label-free detection of endotoxin and gram-negative bacteria from water using copper (I) oxide anchored reduced graphene oxide

Kuttoth, Haritha; Thomas, Teena; Nair, Resmi V.; Sandhyarani, N.

doi:10.1016/j.aca.2022.340597

Cited by 8 publications

(3 citation statements)

References 46 publications

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“…The characteristic anodic and cathodic peaks corresponding to the redox reaction were obtained for BGCE with an I pa of 56.74 μA. For GCE/Cu, the I pa value decreased to 24.68 μA due to the reduction in surface conductivity, as previously documented in a study by, 39 and the ΔE p value increased to 156.25 mV. In the GCE/ ERGO-p(L-Lys) electrode, the I pa value increased to 68.92 μA, and the ΔE p value decreased to 107.42 because the electrochemical reaction of [Fe(CN) 6 ] 3−/4− was supported by the positively charged ERGO-p(L-Lys).…”

Section: Resultssupporting

confidence: 81%

Development of a Cu@ERGO-p(L-Lys) Modified GCE Electrochemical Sensor for the Simultaneous Detection of Ascorbic Acid, Dopamine, and Uric Acid

Atış,

Turan,

Aydoğdu Tığ

2024

J. Electrochem. Soc.

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In this work, a sensitive sensing platform was developed using a glassy carbon electrode (GCE) to simultaneously determine AA, DA, and UA. Cu nanostructures, the poly-L-Lysine (p(L-Lys)), and the electrochemically reduced graphene oxide (ERGO) modified GCE (GCE/Cu@ERGO-p(L-Lys)) was developed via the electrodeposition of Cu and electropolymerization of the ERGO-p(L-Lys). Simultaneous detection makes analysis more efficient and cost-effective by reducing the need for multiple sensors. The GCE/Cu@ERGO-p(L-Lys) was characterized by electrochemical impedance spectroscopy, cyclic voltammetry, and scanning electron microscopy. The limit of detection values for AA, DA, and UA analytes were 0.16, 0.033, and 0.021 μM, respectively, while the linear ranges were 0.53–50.0, 0.11–100.0, and 0.070–0.75 μM. The proposed sensor was found to be applicable for the determination of target analytes in fetal bovine serum samples. The proposed GCE/Cu@ERGO-p(L-Lys) hybrid composite modified electrode is a promising material for simultaneous determination in biological fluids with excellent analytical performance and anti-interference effect.

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Section: Resultssupporting

confidence: 81%

Development of a Cu@ERGO-p(L-Lys) Modified GCE Electrochemical Sensor for the Simultaneous Detection of Ascorbic Acid, Dopamine, and Uric Acid

Atış,

Turan,

Aydoğdu Tığ

2024

J. Electrochem. Soc.

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“…The calculated binding constant value was 3.02 × 10 5 mL g −1 . 44 The binding of PmB−LPS resulted in the detachment of PmB from the CuNCs/CuF surface. Since PmB forms an insulating layer over CuNCs/CuF (shown in Figure 4B), causing a lower current in the presence of PmB, the removal of PmB increases the peak current.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Our group studied the strong binding efficiency of PmB toward LPS by the binding constant calculation. The calculated binding constant value was 3.02 × 10 5 mL g –1 . The binding of PmB–LPS resulted in the detachment of PmB from the CuNCs/CuF surface.…”

Section: Resultsmentioning

confidence: 99%

Electrochemical Approach for the Synthesis of Ultrasmall Cu₁₃ Clusters and Their Application in the Detection of Endotoxin

et al. 2023

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Ultrasmall metal nanoclusters are attractive for their size-dependent optical and electrochemical properties. Here, blue-emitting copper clusters stabilized with cetyltrimethylammonium bromide (CTAB) are synthesized using an electrochemical approach. The electrospray ionization (ESI) analysis reveals that the cluster has 13 copper atoms in the core. The clusters are then used for the electrochemical detection of endotoxina bacterial toxin in Gram-negative bacteria. Differential pulse voltammetry (DPV) is used to detect endotoxins with high selectivity and sensitivity. It shows a lower detection limit of 100 ag mL–1 with a linear range of 100 ag mL–1 to 10 ng mL–1. The sensor is efficient for detecting endotoxins from human blood serum samples.

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Electrochemical sensors for organic and microbial contaminants

Evtugyn,

Kozitsina

2024

Recent Trends and Perspectives on Electrochemical Sensors for Environmental Monitoring

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Label-free detection of endotoxin and gram-negative bacteria from water using copper (I) oxide anchored reduced graphene oxide

Cited by 8 publications

References 46 publications

Development of a Cu@ERGO-p(L-Lys) Modified GCE Electrochemical Sensor for the Simultaneous Detection of Ascorbic Acid, Dopamine, and Uric Acid

Development of a Cu@ERGO-p(L-Lys) Modified GCE Electrochemical Sensor for the Simultaneous Detection of Ascorbic Acid, Dopamine, and Uric Acid

Electrochemical Approach for the Synthesis of Ultrasmall Cu₁₃ Clusters and Their Application in the Detection of Endotoxin

Electrochemical sensors for organic and microbial contaminants

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Label-free detection of endotoxin and gram-negative bacteria from water using copper (I) oxide anchored reduced graphene oxide

Cited by 8 publications

References 46 publications

Development of a Cu@ERGO-p(L-Lys) Modified GCE Electrochemical Sensor for the Simultaneous Detection of Ascorbic Acid, Dopamine, and Uric Acid

Development of a Cu@ERGO-p(L-Lys) Modified GCE Electrochemical Sensor for the Simultaneous Detection of Ascorbic Acid, Dopamine, and Uric Acid

Electrochemical Approach for the Synthesis of Ultrasmall Cu13 Clusters and Their Application in the Detection of Endotoxin

Electrochemical sensors for organic and microbial contaminants

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Electrochemical Approach for the Synthesis of Ultrasmall Cu₁₃ Clusters and Their Application in the Detection of Endotoxin