Label-free Electrochemical Cocaine Aptasensor Based on a Target-inducing Aptamer Switching Conformation

Mei, Hua; Tao, Manlan; Wang, Ping; Zhang, Yinfeng; Wu, Zai‐Sheng; Chang, Yanbing; Yang, Yunhui

doi:10.2116/analsci.26.1265

Cited by 34 publications

(15 citation statements)

References 28 publications

(22 reference statements)

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“…In aptasensors based on competitive replacement [33], the aptamer is immobilized on the sensor surface. Before the measurement of the analyte, the labelled target is bound to the aptamer.…”

Section: Aptasensors Based On Competitive Replacementmentioning

confidence: 99%

Aptasensors for Small Molecule Detection

Walter

Heilkenbrinker

Austerjost

et al. 2012

Zeitschrift Für Naturforschung B

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Dedicated to Professor Heribert Offermanns on the occasion of his 75 th birthdayAptamers are single-stranded oligonucleotides composed of RNA or DNA that are able to bind their corresponding targets via molecular recognition. Thus, aptamers can be thought of as nucleic acidbased alternatives to antibodies and have attracted attention as receptors in biosensors. Aptamers seem to be ideal biological recognition elements, since they enable the design of intelligent sensors based on their specific properties. Especially the fact that most aptamers undergo conformational changes during the binding of the target and their oligonucleotide nature can be used to rationally design novel sensing strategies. This review focuses on aptasensors for the detection of small molecules. In the first part, aptamers, their generation and their properties are briefly described. In the second part, different design strategies for aptasensors are reviewed, and examples for the detection of small molecules are given.

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“…In aptasensors based on competitive replacement [33], the aptamer is immobilized on the sensor surface. Before the measurement of the analyte, the labelled target is bound to the aptamer.…”

Section: Aptasensors Based On Competitive Replacementmentioning

confidence: 99%

Aptasensors for Small Molecule Detection

Walter

Heilkenbrinker

Austerjost

et al. 2012

Zeitschrift Für Naturforschung B

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“…Generally, in water algal bloom, MC-LR may coexist with some algal toxins such as Nodularin-R (NODLR), MC-RR, MC-YR, 17β-estradiol (EE2), as well as some food toxins such as zearalenone (ZEO) [38,39,40,41]. Therefore, there is a need to evaluate the cross-reactivity of the aptatoxisensor.…”

Section: Resultsmentioning

confidence: 99%

Electrochemical Aptatoxisensor Responses on Nanocomposites Containing Electro-Deposited Silver Nanoparticles on Poly(Propyleneimine) Dendrimer for the Detection of Microcystin-LR in Freshwater

Bilibana

Williams

Rassie

et al. 2016

Sensors

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A sensitive and reagentless electrochemical aptatoxisensor was developed on cobalt (II) salicylaldiimine metallodendrimer (SDD–Co(II)) doped with electro-synthesized silver nanoparticles (AgNPs) for microcystin-LR (L, l-leucine; R, l-arginine), or MC-LR, detection in the nanomolar range. The GCE|SDD–Co(II)|AgNPs aptatoxisensor was fabricated with 5’ thiolated aptamer through self-assembly on the modified surface of the glassy carbon electrode (GCE) and the electronic response was measured using cyclic voltammetry (CV). Specific binding of MC-LR with the aptamer on GCE|SDD–Co(II)|AgNPs aptatoxisensor caused the formation of a complex that resulted in steric hindrance and electrostatic repulsion culminating in variation of the corresponding peak current of the electrochemical probe. The aptatoxisensor showed a linear response for MC-LR between 0.1 and 1.1 µg·L−1 and the calculated limit of detection (LOD) was 0.04 µg·L−1. In the detection of MC-LR in water samples, the aptatoxisensor proved to be highly sensitive and stable, performed well in the presence of interfering analog and was comparable to the conventional analytical techniques. The results demonstrate that the constructed MC-LR aptatoxisensor is a suitable device for routine quantification of MC-LR in freshwater and environmental samples.

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“…Reported detection limits of cocaine in other studies were as following: 190 nM for label-free fluorescence aptamer-based sensor (Qiu et al 2013), 3 ng/mL for HPLC/MS (Johansen and Bhatia 2007), 0.5 pM for Aptamer-based microfluidic beads array sensor (Zhang et al 2014), 300 nM for Label-free electrochemical cocaine aptasensor (Hua et al 2010), 1 ng/mL for reversed-phase HPLC (Tagliaro et al 1994), 5 µM for label-free DNA hairpin biosensor for colorimetric detection (Nie et al 2013), 10 pM for microfluidic affinity sensor (Hilton et al 2011), 0.1 μM for Solid-state probe based electrochemical aptasensor (Du et al 2010), 0.48 nM for chemiluminescence aptasensor based on double-functionalized gold nanoprobes and functionalized magnetic microbeads ) and down to 20 nM for electrochemical aptasensor based on enzyme linked aptamer assay (Zhang et al 2012). In comparison with the designed electrochemical aptasensor most of these methods are expensive, time-consuming and have higher LODs.…”

Section: Cocaine Analysismentioning

confidence: 90%