Label-free electrochemical immunosensor based on Nile blue A-reduced graphene oxide nanocomposites for carcinoembryonic antigen detection

Gao, Yansha; Zhu, Xiaofei; Xu, Jingkun; Lu, Limin; Wang, Wenmin; Yang, Taotao; Xing, Huakun; Yu, Yongfang

doi:10.1016/j.ab.2016.02.010

Cited by 61 publications

(26 citation statements)

References 38 publications

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“…Using carcinoembryonic antigen (CEA) as a model analyte, we developed a label-free photoelectrochemical immunosensor for detection of CEA based on 2D TiO 2 nanosheets and carboxylated graphitic carbon nitride. CEA, a usual tumor marker, can be used for the early detection of recurrent diseases and indicate the effect of therapy in early breast cancer and gastrointestinal cancers as well as other tumor markers1920. Coupling carboxylated graphitic carbon nitride with 2D TiO 2 nanosheets can evidently extend the absorption range, increase the utilization of light energy, and promote the photocurrent intensity.…”

mentioning

confidence: 99%

Photoelectrochemical Immunosensor for Detection of Carcinoembryonic Antigen Based on 2D TiO2 Nanosheets and Carboxylated Graphitic Carbon Nitride

Wang

Zhang

et al. 2016

Sci Rep

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Carcinoembryonic antigen (CEA) was used as the model, an ultrasensitive label-free photoelectrochemical immunosensor was developed using 2D TiO2 nanosheets and carboxylated graphitic carbon nitride (g-C3N4) as photoactive materials and ascorbic acid as an efficient electron donor. 2D TiO2 nanosheets was sythsized by surfactant self-assembly method and proved to have higher photoelectrochemical signals than TiO2 nanoparticles. Firstly, carboxylated g-C3N4 could be attached to 2D TiO2 nanosheets through the bond formed between carboxyl group of carboxylated g-C3N4 and TiO2. And the photocurrent of g-C3N4/TiO2 drastically enhances compared to carboxylated g-C3N4 and TiO2. Then, antibody of CEA was bonded to TiO2 through the dentate bond formed between carboxyl group of anti-CEA and TiO2, leading to the decrease of the photocurrents. As proven by PEC experiments and electrochemical impedance spectroscopy (EIS) analysis, the fabrication process of the immunosensor is successful. Under the optimal conditions, the intensity decreased linearly with CEA concentration in the range of 0.01~10 ng/mL. The detection limit is 2.1 pg/mL. The work provides an effective method for the detection of tumor markers and can be extended for the application in food safety and environmental monitoring analysis.

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mentioning

confidence: 99%

Photoelectrochemical Immunosensor for Detection of Carcinoembryonic Antigen Based on 2D TiO2 Nanosheets and Carboxylated Graphitic Carbon Nitride

Wang

Zhang

et al. 2016

Sci Rep

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“…This immunosensor offered a linear signal between 1 pg mL −1 and 30 ng mL −1 , with a LoD of 0.4 pg mL −1 . Last example for CEA detection, Gao et al [80] described a Nile blue–modified GO which was electrochemically reduce in the presence of AuCl 4 − , to led to a AuNPs/NB/RGO-modified electrode. CEA antibodies were immobilized on this nanocomposite.…”

Section: Discussionmentioning

confidence: 99%

Recent Advances in Electrochemical Immunosensors

Piro

Reisberg

2017

Sensors

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Immunosensors have experienced a very significant growth in recent years, driven by the need for fast, sensitive, portable and easy-to-use devices to detect biomarkers for clinical diagnosis or to monitor organic pollutants in natural or industrial environments. Advances in the field of signal amplification using enzymatic reactions, nanomaterials such as carbon nanotubes, graphene and graphene derivatives, metallic nanoparticles (gold, silver, various oxides or metal complexes), or magnetic beads show how it is possible to improve collection, binding or transduction performances and reach the requirements for realistic clinical diagnostic or environmental control. This review presents these most recent advances; it focuses first on classical electrode substrates, then moves to carbon-based nanostructured ones including carbon nanotubes, graphene and other carbon materials, metal or metal-oxide nanoparticles, magnetic nanoparticles, dendrimers and, to finish, explore the use of ionic liquids. Analytical performances are systematically covered and compared, depending on the detection principle, but also from a chronological perspective, from 2012 to 2016 and early 2017.

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“…During the testing process, the FDA also identified CEA as an acceptable serum biomarker for colon cancer [3]. For detection of CEA, numerous electrochemical immunosensors have been developed [4][5][6][7].…”

Section: Carcinoembryonic Antigen (Cea)mentioning

confidence: 99%

“…The decrease in the electrical signals of the immunosensor has a linear relationship for the quantitative detection of CEA ranging from 100 fg mL −1 to 400 ng mL −1 with a low detection limit of 100 fg mL −1 . Interestingly, a novel label-free electrochemical immunosensors [5] for detecting CEA based on gold nanoparticles (AuNPs) and Nile blue A (NB) hybridized electrochemically reduced graphene oxide (NB-ERGO) as shown in Figure 2. The NB-graphene oxide (NB-GO) composite was developed by the π-π interaction.…”

Section: Carcinoembryonic Antigen (Cea)mentioning

confidence: 99%

Current and Prospective of Breast Cancer Biomarkers

Benjamin¹,

Lima²

2021

Molecular Biotechnology

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Biomarkers have shown great promise over the past decade the process of drug development more effective and have become an integral part of diagnosis of diseases. Biosensors were integrated with biomarker detection and point-of-care detection for signal amplification, high specificity and sensitivity, rapid response time, low cost, simplicity and multi-analytical testing. In order to detect more sensitively, these particular biomarkers have been explored with the possibility of real-time measurements in order to develop simple and compact systems which can analyze complex specimens. Various biosensors including electrochemical biosensors have recently been developed based on disease-specific biomarkers in the diagnosis of cancer disease. The main objective of the book chapter is to review research with new materials/methods in electrochemical biosensing techniques to detection of breast cancer biomarkers and evaluating latest techniques for detection of important analytes in real samples. In this book chapter, the recent development of electrochemical biosensors of breast cancer biomarkers will be reviewed. Furthermore, recent and future trend application of breast cancer biomarkers will be discussed.

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Label-free electrochemical immunosensor based on Nile blue A-reduced graphene oxide nanocomposites for carcinoembryonic antigen detection

Cited by 61 publications

References 38 publications

Photoelectrochemical Immunosensor for Detection of Carcinoembryonic Antigen Based on 2D TiO2 Nanosheets and Carboxylated Graphitic Carbon Nitride

Photoelectrochemical Immunosensor for Detection of Carcinoembryonic Antigen Based on 2D TiO2 Nanosheets and Carboxylated Graphitic Carbon Nitride

Recent Advances in Electrochemical Immunosensors

Current and Prospective of Breast Cancer Biomarkers

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