Label-free electrochemical immunosensor for the rapid and sensitive detection of the oxidative stress marker superoxide dismutase 1 at the point-of-care

Santharaman, Paulraj; Das, Mainak; Singh, Sushil Kumar; Sethy, Niroj Kumar; Bhargava, Kalpana; Claussen, Jonathan C.; Karunakaran, C.

doi:10.1016/j.snb.2016.06.040

Cited by 29 publications

(8 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As some markers of oxidative stress, along with indicators of antioxidant defense, are available as point-of-care test (POCT), the confirmation of a relationship between oxidative stress markers and sepsis severity would be of major relevance in critical care settings. Particularly, available or under development POCTs for oxidative stress and antioxidant status include free oxygen radicals test [ 56 , 57 ], free oxygen radicals defense test [ 56 , 57 ], 8-hydroxy-2′-deoxyguanosine [ 58 ], 3-nitrotyrosine [ 59 ], CuZn SOD [ 60 ], BAP, measured as capacity of reduction of ferric to ferrous ions [ 57 ], and iridium-reducing assay, particularly sensitive to GSH [ 61 ]. Despite at present these POCTs have been studied in the adult population, they would offer several advantages for critically ill newborns, as timely manner stratification of sepsis severity and identification of a patient who would particularly benefit from antioxidant strategies, the need for small blood samples, and the possibility to monitor the response to antioxidant treatment.…”

Section: Redox Status In Neonatal Sepsismentioning

confidence: 99%

Sepsis and Oxidative Stress in the Newborn: From Pathogenesis to Novel Therapeutic Targets

Poggi

Dani

2018

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

Sepsis is at present one of the leading causes of morbidity and mortality in the neonatal population. Together with inflammation, oxidative stress is involved in detrimental pathways activated during neonatal sepsis, eventually leading to organ dysfunction and death. The redox cascade during sepsis is mainly initiated by IL-6 and IL-8 stimulation in newborns and includes multiple noxious processes, as direct cell damage induced by reactive oxygen species, activation of gene expression leading to amplification of inflammation and oxidative stress, and impairment of mitochondrial function. Once proinflammatory and prooxidant pathways are established as stimulated by causing pathogens, self-maintaining unfavorable redox cycles ensue, leading to oxidative stress-related cellular damage, independently from the activating pathogens themselves. Despite antioxidant systems are induced during neonatal sepsis, as an adaptive response to an increased oxidative burden, a condition of redox imbalance favoring oxidative pathways occurs, resulting in increased markers of oxidative stress damage. Therefore, antioxidant treatment would exert beneficial effects during neonatal sepsis, potentially interrupting prooxidant pathways and preventing the maintenance of detrimental redox cycles that cannot be directly affected by antibiotic treatment. Among others, antioxidant agents investigated in clinical settings as adjunct treatment for neonatal sepsis include melatonin and pentoxifylline, both showing promising results, while novel antioxidant molecules, as edaravone and endothelin receptor antagonists, are at present under investigation in animal models. Finally, mitochondria-targeted antioxidant treatments could represent an interesting line of research in the treatment of neonatal sepsis.

show abstract

Section: Redox Status In Neonatal Sepsismentioning

confidence: 99%

Sepsis and Oxidative Stress in the Newborn: From Pathogenesis to Novel Therapeutic Targets

Poggi

Dani

2018

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

show abstract

“…Graphitic materials are known for their good structural, electronic, mechanical, optical, thermal and chemical properties 42 . In the last years, carbon-based nanomaterials, such as, graphene 43 , nanotubes 44 , nanoparticles 45 and conductive polymers 46 are being widely employed for biosensing devices. Due to their high surface area and high electrical conductivity, these electrochemical sensors have become a potential alternative to the conventional labour intensive and time consuming assays that are currently used for biomarker analysis.…”

Section: Resultsmentioning

confidence: 99%

Paper-Based Sensing Device for Electrochemical Detection of Oxidative Stress Biomarker 8-Hydroxy-2′-deoxyguanosine (8-OHdG) in Point-of-Care

Martins

Tavares

Fortunato

et al. 2017

Sci Rep

View full text Add to dashboard Cite

This work presents a cost-effective, label-free in point-of-care (POC) biosensor for the sensitive detection of 8-hydroxy-2′-deoxyguanosine (8-OHdG), the most abundant oxidative product of DNA, that may allow a premature assessment of cancer disease, thereby improving diagnosis, prognostics and survival rates. The device targets the direct detection of 8-OHdG by using for the first time a carbon-ink 3-electrode on a paper substrate coupled to Differential Pulse Voltammetry readings. This design was optimized by adding nanostructured carbon materials to the ink and the conducting polymer PEDOT, enhancing the electrocatalytic properties of the sensor towards 8-OHdG detection. Meanwhile, the ability of this oxidative stress biomarker to undertake an oxidation reaction enabled the development of the sensing electrochemical device without the need of chemical probes and long incubation periods. This paper-modified sensor presented high electrochemical performance on the oxidation of 8-OHdG with a wide linear range (50–1000 ng/ml) and a low detection limit (14.4 ng/ml). Thus, our results showed the development of a direct and facile sensor with good reproducibility, stability, sensitivity and more importantly, selectivity. The proposed carbon-based electrochemical sensor is a potential candidate to be miniaturized to small portable size, which make it applicable for in-situ 8-OHdG sensing in real biological samples.

show abstract

“…The electrochemical oxygen probe showed a detection limit of 1.5 x 10 -9 M, 46 and two assays using surface plasma resonance (SPR) had detection limits of 1 x 10 -9 M 47 and 0.9 x 10 -6 M. 48 The chromogenic ELISA employing HRP-antibody conjugate and 3,3',5,5'-tetramethylbenzidine gave a detection limit of 1.2 x 10 -6 M and requires at least 6 h. 48 The most recent electrochemical method for detection of superoxide dismutase using affinity interaction has detection limit of 0.5 nM. 49 Thus, our fluorogenic and electrochemical assays requiring between 4 and 6 h of total incubation and washing time improve upon these published detection limits by three orders of magnitude. This remarkable decrease in the detection limit will allow for smaller samples needed from patients.…”

Section: Scheme 3 Electrochemical Detection Methods Using Screen Prinmentioning

confidence: 99%

Microbead QD-ELISA: Microbead ELISA Using Biocatalytic Formation of Quantum Dots for Ultra High Sensitive Optical and Electrochemical Detection

Grinyte

Barroso

Möller

et al. 2016

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Electrochemical detection strategies employing semiconductor quantum dots (QDs) open up new opportunities for highly sensitive detection of biological targets. We designed a new assay based on microbead linked enzymatic generation of CdS QDs (Microbead QD-ELISA) and employed it in optical and electrochemical affinity assays for the cancer biomarker superoxide dismutase 2 (SOD2). Biotinylated antibodies against SOD2 were immobilized on the surface of polyvinyl chloride microbeads bearing streptavidin. In order to prevent any non-specific adsorption the microbeads were further blocked with bovine serum albumin. The analyte, SOD2 was captured on microbeads and labeled with alkaline phosphatase-conjugated antibody linked with mouse antibody against SOD2. Hydrolysis of para-nitrophenylphosphate by immobilized alkaline phosphatase triggered the rapid formation of phosphate-stabilized CdS QDs on the surface of microbeads. The resulting semiconductor nanoparticles were detected by fluorescence spectroscopy, microscopy, and square-wave voltammetry (SWV). The electrochemical assay based on the detection with square-wave voltammograms of Cd ions originating from immobilized CdS QDs showed linearity up to 45 ng mL, and the limit of SOD2 detection equal to 0.44 ng mL (1.96 × 10 M). This detection limit is lower by 2 orders of magnitude in comparison with that of other previously published assays for superoxide dismutase. The electrochemical assay was validated with HepG2 (Human hepatocellular carcinoma) cell lysate containing SOD2.

show abstract

Label-free electrochemical immunosensor for the rapid and sensitive detection of the oxidative stress marker superoxide dismutase 1 at the point-of-care

Cited by 29 publications

References 49 publications

Sepsis and Oxidative Stress in the Newborn: From Pathogenesis to Novel Therapeutic Targets

Sepsis and Oxidative Stress in the Newborn: From Pathogenesis to Novel Therapeutic Targets

Paper-Based Sensing Device for Electrochemical Detection of Oxidative Stress Biomarker 8-Hydroxy-2′-deoxyguanosine (8-OHdG) in Point-of-Care

Microbead QD-ELISA: Microbead ELISA Using Biocatalytic Formation of Quantum Dots for Ultra High Sensitive Optical and Electrochemical Detection

Contact Info

Product

Resources

About