Label‐Free Homogeneous Immunosensor Based on FRET for the Detection of Virus Antibody in Serum

Zhang, Cuiling; Gao, Dingshan; Zhou, Guohua; Lu, Chen; Zhang, Xianan; Cui, Zongqiang; He, Zhike

doi:10.1002/asia.201200221

Cited by 10 publications

(7 citation statements)

References 40 publications

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“…Using a similar concept, a label-free homogeneous immunosensor for the detection of the simian vacuolating virus 40 (SV40) was developed. 802 QDs coated with SV40 virus-like particles (SVLP) were adsorbed to the surface of GO through hydrophobic interactions leading to ET and quenching of the QD PL. The addition of an anti-SV40 antibody created a larger complex with the SVLP-coated QDs that increased the distance between the QD and GO, reducing ET.…”

Section: Sensorsmentioning

confidence: 99%

Energy Transfer with Semiconductor Quantum Dot Bioconjugates: A Versatile Platform for Biosensing, Energy Harvesting, and Other Developing Applications

Spillmann²,

et al. 2016

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Luminescent semiconductor quantum dots (QDs) are one of the more popular nanomaterials currently utilized within biological applications. However, what is not widely appreciated is their growing role as versatile energy transfer (ET) donors and acceptors within a similar biological context. The progress made on integrating QDs and ET in biological configurations and applications is reviewed in detail here. The goal is to provide the reader with (1) an appreciation for what QDs are capable of in this context, (2) how this field has grown over a relatively short time span, and, in particular, (3) how QDs are steadily revolutionizing the development of new biosensors along with a myriad of other photonically active nanomaterial-based bioconjugates. An initial discussion of QD materials along with key concepts surrounding their preparation and bioconjugation is provided given the defining role these aspects play in the QDs ability to succeed in subsequent ET applications. The discussion is then divided around the specific roles that QDs provide as either Förster resonance energy transfer (FRET) or charge/electron transfer donor and/or acceptor. For each QD-ET mechanism, a working explanation of the appropriate background theory and formalism is articulated before examining their biosensing and related ET utility. Other configurations such as incorporation of QDs into multistep ET processes or use of initial chemical and bioluminescent excitation are treated similarly. ET processes that are still not fully understood such as QD interactions with gold and other metal nanoparticles along with carbon allotropes are also covered. Given their maturity, some specific applications ranging from in vitro sensing assays to cellular imaging are separated and discussed in more detail. Finally a perspective on how this field will continue to evolve is provided.

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Section: Sensorsmentioning

confidence: 99%

Energy Transfer with Semiconductor Quantum Dot Bioconjugates: A Versatile Platform for Biosensing, Energy Harvesting, and Other Developing Applications

Spillmann²,

et al. 2016

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“…An original approach relies on an immunosensor made of GO and quantum dots encapsulated in simian vacuolating virus 40 (SV40) virus-like particles, which was exploited for the detection of anti-SV40 antibodies in serum. 202 SV40 is a nonenveloped tumorigenic virus, and the occurrence of nucleic acid sequences identical with SV40 sequences was observed in some tumors. As a result, the detection of serum anti-SV40 antibodies allows the diagnosis of cancer related to SV40.…”

Section: ■ Detection Of Viruses and Proteins Released By Infected Org...mentioning

confidence: 99%

Two-Dimensional Material-Based Biosensors for Virus Detection

2020

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Viral infections are one of the major causes of mortality and economic losses worldwide. Consequently, efficient virus detection methods are crucial to determine the infection prevalence. However, most detection methods face challenges related to false-negative or falsepositive results, long response times, high costs, and/or the need for specialized equipment and staff. Such issues can be overcome by access to low-cost and fast response point-of-care detection systems, and two-dimensional materials (2DMs) can play a critical role in this regard. Indeed, the unique and tunable physicochemical properties of 2DMs provide many advantages for developing biosensors for viral infections with high sensitivity and selectivity. Fast, accurate, and reliable detection, even at early infection stages by the virus, can be potentially enabled by highly accessible surface interactions between the 2DMs and the analytes. High selectivity can be obtained by functionalization of the 2DMs with antibodies, nucleic acids, proteins, peptides or aptamers, allowing for specific binding to a particular virus, viral fingerprints or proteins released by the host organism. Multiplexed detection and discrimination between different virus strains are also feasible. In this review, we present a comprehensive overview of the major advances of 2DMbased biosensors for the detection of viruses. We describe the main factors governing the efficient interactions between viruses and 2DMs, making them ideal candidates for the detection of viral infections. We also critically detail their advantages and drawbacks, providing insights for the development of future biosensors for virus detection. Lastly, we provide suggestions to stimulate research in the fast expanding field of 2DMs that could help in designing advanced systems for preventing virus-related pandemics.

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“…The energy was transferred from SVLP-QD to GO, and the constructed label-free immunosensing platform could be used for rapid, inexpensive, and easy-to-perform detection of anti-SV40 antibody directly in serum. 156 Until now, several kinds of immunoassay systems including electrochemical and colorimetric ones were explored based on intrinsic properties of graphene, e.g., performing a central role in fast electron-transfer kinetics as well as promoting further signal amplication, for achieving high detection sensitivity, wide detection range, good selectivity and low detection limit. In the next step, real-life applications, such as cancer diagnostics, of these novel immunosensors prepared by graphene hybrids and composites integrated with both organic and inorganic materials should be carried out.…”

Section: Biosensing and Immunosensingmentioning

confidence: 99%

Recent advancements of graphene in biomedicine

2013

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Graphene, as a rising star in the field of nanomaterials, possesses a unique planar structure and exceptional electronic, mechanical, and optical properties. The material has attracted tremendous interest not only for its intrinsic properties but also promising application opportunities in a wide range of technologies and markets. This review specifically summarizes recent research advancements of graphene in the areas of biotechnology and biomedicine. The bio-application opportunities lie in the unique attributes of graphene, i.e., (1) its nano-scale structure that allows for bio-compatibility, (2) biofunctionalization of graphene for biological recognition, (3) its mechanical, electronic and optical properties for bioimaging and external stimulus driven therapeutics, and (4) its functionalities for tissue and genetic engineering.The review mainly highlights eight subjects including (1) biocompatibility, cytotoxicity and biofunctionalization, (2) antibacterial activity, (3) biosensing and immunosensing, (4) bioimaging, (5) genetic engineering, (6) drug delivery, (7) cancer phototherapy, and (8) tissue engineering. Perspectives and future challenges in this rapidly developing area are also discussed.

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Label‐Free Homogeneous Immunosensor Based on FRET for the Detection of Virus Antibody in Serum

Cited by 10 publications

References 40 publications

Energy Transfer with Semiconductor Quantum Dot Bioconjugates: A Versatile Platform for Biosensing, Energy Harvesting, and Other Developing Applications

Energy Transfer with Semiconductor Quantum Dot Bioconjugates: A Versatile Platform for Biosensing, Energy Harvesting, and Other Developing Applications

Two-Dimensional Material-Based Biosensors for Virus Detection

Recent advancements of graphene in biomedicine

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