Label-free immunodetection of α-synuclein by using a microfluidics coplanar electrolyte-gated organic field-effect transistor

Ricci, Simona; Casalini, Stefano; Parkula, Vitaliy; Selvaraj, Meenu; Saygin, Gulseren Deniz; Greco, Pierpaolo; Biscarini, Fabio; Mas‐Torrent, Marta

doi:10.1016/j.bios.2020.112433

Cited by 59 publications

(66 citation statements)

References 60 publications

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“…[ 62,63 ] Typically, in EGOFETs water is used as electrolyte in order to apply these devices in biosensing. [ 64–67 ] However, one of their main drawbacks is the stability of the devices in such aqueous environment. Previously, we demonstrated that the use of OSC:PS blends leads to EGOFETs with an improved stability.…”

Section: Resultsmentioning

confidence: 99%

Organic Field‐Effect Transistors Based on Ternary Blends Including a Fluorinated Polymer for Achieving Enhanced Device Stability

Tamayo

Salzillo

Mas‐Torrent

2022

Adv Materials Inter

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The stability of organic semiconductors (OSCs) is strongly hampered by the presence of water molecules. One approach that has been proved to lead to organic field‐effect transistors with an enhanced performance is the use of blends of OSCs with insulating binding polymers. In this work, the fabrication of OSC thin films based on polymeric ternary blends including a hydrophobic fluorinated polymer is reported as a novel route to engineer long‐term reliable organic field‐effect transistors (OFET) devices. In particular, OFETs based on blends of bis(triisopropylsilylethynyl)pentacene (TIPS) with polystyrene (PS) and poly(pentafluorostyrene) (PFS) are explored. The PS:PFS ratio is tuned in order to find the optimum formulation. It is shown that films including 20% of PFS in the polymeric blend exhibit an improved device performance, which is reflected by a low bias stress and an exceptional environmental stability, without significantly hampering the OFET mobility. This work advocates that adding a small percentage of fluorinated polymers in OSC blends is a promising route to realize more reliable and stable devices without importantly compromising the device mobility.

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Section: Resultsmentioning

confidence: 99%

Organic Field‐Effect Transistors Based on Ternary Blends Including a Fluorinated Polymer for Achieving Enhanced Device Stability

Tamayo

Salzillo

Mas‐Torrent

2022

Adv Materials Inter

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“…106 Copyright 2016, Springer Casalini et al also integrated microfluidics into an EG-OFET for the detection of α-synuclein at levels from 0.25 pM to 25 nM. 110 Such microfluidics engineering is important for controlling the functionalization of gate electrodes, as well as preventing contamination or physisorption on the OSC. Sabate et al constructed an EG-OFET-based sensor using anti-human immunodeficiency virus (HIV)-1 p24 capsid protein functionalized gate for the detection of HIV-1 p24 proteins at levels around 1 fM (Figure 14).…”

Section: Eg-ofet-based Liquid Sensorsmentioning

confidence: 99%

Recent progress in organic field‐effect transistor‐based chem/bio‐sensors

Luo

Liu

2022

VIEW

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Recent decades have witnessed the huge successes of organic field‐effect transistors (OFETs) and their applications. Among them, OFET‐based sensors have shown promising applications in the field of food safety, industrial security, environmental, and health monitoring. This is due to their advantages in solution processability, flexibility, light weight, and variety in molecular design. This review highlights recent progress in OFET‐based chemical and biological sensors in gas and liquid phase, especially for the past 5 years. The analytes range from small molecules to large biomolecules. The optimizations of OFET devices for sensors, including the semiconducting layers, dielectric layers, electrodes, and their interfaces etc., are illustrated. And their relationships with sensing parameters (sensitivity, selectivity, and response time) as well as the sensing mechanisms are given. Finally, the remaining challenges are discussed. We expect that this review can offer inspiration for future design of OFET‐based sensors.

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“…Another important aspect to be considered is that this design can be potentially exploited also to run the in-situ functionalization of the gate electrode [44]. This realization would be a fundamental step towards the commercial exploitation of this sensing technologies, since it would overcome the practical limitation of a pre-functionalization of the gate electrode, with the consequent stability and stocking issues [45,46].…”

Section: Design and Simulationmentioning

confidence: 99%

Design of a Portable Microfluidic Platform for EGOT-Based in Liquid Biosensing

Segantini

Parmeggiani

Ballesio

et al. 2022

Sensors

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In biosensing applications, the exploitation of organic transistors gated via a liquid electrolyte has increased in the last years thanks to their enormous advantages in terms of sensitivity, low cost and power consumption. However, a practical aspect limiting the use of these devices in real applications is the contamination of the organic material, which represents an obstacle for the realization of a portable sensing platform based on electrolyte-gated organic transistors (EGOTs). In this work, a novel contamination-free microfluidic platform allowing differential measurements is presented and validated through finite element modeling simulations. The proposed design allows the exposure of the sensing electrode without contaminating the EGOT device during the whole sensing tests protocol. Furthermore, the platform is exploited to perform the detection of bovine serum albumin (BSA) as a validation test for the introduced differential protocol, demonstrating the capability to detect BSA at 1 pM concentration. The lack of contamination and the differential measurements provided in this work can be the first steps towards the realization of a reliable EGOT-based portable sensing instrument.

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Label-free immunodetection of α-synuclein by using a microfluidics coplanar electrolyte-gated organic field-effect transistor

Cited by 59 publications

References 60 publications

Organic Field‐Effect Transistors Based on Ternary Blends Including a Fluorinated Polymer for Achieving Enhanced Device Stability

Organic Field‐Effect Transistors Based on Ternary Blends Including a Fluorinated Polymer for Achieving Enhanced Device Stability

Recent progress in organic field‐effect transistor‐based chem/bio‐sensors

Design of a Portable Microfluidic Platform for EGOT-Based in Liquid Biosensing

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