Label-free lateral flow assay for Listeria monocytogenes by aptamer-gated release of signal molecules

Taşbaşi, Behiye Büşra; Guner, Buket C.; Sudağıdan, Mert; Uçak, Samet; Kavruk, Murat; Özalp, Veli Cengiz

doi:10.1016/j.ab.2019.113449

Cited by 52 publications

(19 citation statements)

References 23 publications

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“…The high catalytic activity of HRP has led to its application in the LFA to improve the assay's sensitivity. Ozalp's group [ 95 ] immobilized HRP in the test zone of an LFA to indicate whether the target was present. In that study, after the interaction between the target bacterial cells and the aptamer in the conjugation zone of the LFA, TMB was released and migrated to test zone, where HRP converted the free TMB into blue particles.…”

Section: Labeling Materials Used In Lateral Flow Assaysmentioning

confidence: 99%

An Overview for the Nanoparticles‐Based Quantitative Lateral Flow Assay

Wang

Zhao

et al. 2021

Small Methods

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The development of the lateral flow assay (LFA) has received much attention in both academia and industry because of their broad applications to food safety, environmental monitoring, clinical diagnosis, and so forth. The user friendliness, low cost, and easy operation are the most attractive advantages of the LFA. In recent years, quantitative detection has become another focus of LFA development. Here, the most recent studies of quantitative LFAs are reviewed. First, the principles and corresponding formats of quantitative LFAs are introduced. In the biomaterial and nanomaterial sections, the detection, capture, and signal amplification biomolecules and the optical, fluorescent, luminescent, and magnetic labels used in LFAs are described. The invention of dedicated strip readers has drawn further interest in exploiting the better performance of LFAs. Therefore, next, the development of dedicated reader devices is described and the usefulness and specifications of these devices for LFAs are discussed. Finally, the applications of LFAs in the detection of metal ions, biotoxins, pathogenic microorganisms, veterinary drugs, and pesticides in the fields of food safety and environmental health and the detection of nucleic acids, biomarkers, and viruses in clinical analyses are summarized.

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Section: Labeling Materials Used In Lateral Flow Assaysmentioning

confidence: 99%

An Overview for the Nanoparticles‐Based Quantitative Lateral Flow Assay

Wang

Zhao

et al. 2021

Small Methods

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“…The production, labeling, amplification, and modification processes are also easier in aptamers in comparison to antibodies. Recently, aptamer‐functionalized AuNPs and aptamer‐gated silica NPs have been used to improve the sensitivity of LFA (Dalirirad & Steckl, 2019; Tasbasi et al., 2019).…”

Section: Poc Testing Devicesmentioning

confidence: 99%

Rapid point‐of‐care testing methods/devices for meat species identification: A review

Kumar

Narsaiah

2020

Comp Rev Food Sci Food Safe

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The authentication of animal species is an important issue due to an increasing trend of adulteration and mislabeling of animal species in processed meat products. Polymerase chain reaction is the most sensitive and specific technique for nucleic acid‐based animal species detection. However, it is a time‐consuming technique that requires costly thermocyclers and sophisticated labs. In recent times, there is a need of on‐site detection by point‐of‐care (POC) testing methods and devices under low‐resource settings. These POC devices must be affordable, sensitive, specific, user‐friendly, rapid and robust, equipment free, and delivered to the end users. POC devices should also confirm the concept of micro total analysis system. This review discusses POC testing methods and devices that have been developed for meat species identification. Recent developments in lateral flow assay‐based devices for the identification of animal species in meat products are also reviewed. Advancements in increasing the efficiency of lateral flow detection are also discussed.

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“…dynamic light scattering immunoassay, lateral-flow enzyme immunoconcentration, enzyme-linked immunosorbent assay and quartz crystal microbalance immunosensor) have been developed for LM detection. 4,[8][9][10][11] There is no doubt that these method were very important in detecting LM, while they are easy to suffer from some defects either time-consuming, expensive equipment or complex operation. Compared to the general methods, the electrochemical technology has many incomparable merits since it's very simple, low-cost, easy to operate, and time-saving.…”

Section: Introductionmentioning

confidence: 99%

A novel electrochemical sandwich-like immunosensor based on carboxyl Ti₃C₂T_x MXene and rhodamine b/gold/reduced graphene oxide for Listeria monocytogenes

et al. 2022

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Label-free lateral flow assay for Listeria monocytogenes by aptamer-gated release of signal molecules

Cited by 52 publications

References 23 publications

An Overview for the Nanoparticles‐Based Quantitative Lateral Flow Assay

An Overview for the Nanoparticles‐Based Quantitative Lateral Flow Assay

Rapid point‐of‐care testing methods/devices for meat species identification: A review

A novel electrochemical sandwich-like immunosensor based on carboxyl Ti₃C₂T_x MXene and rhodamine b/gold/reduced graphene oxide for Listeria monocytogenes

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Label-free lateral flow assay for Listeria monocytogenes by aptamer-gated release of signal molecules

Cited by 52 publications

References 23 publications

An Overview for the Nanoparticles‐Based Quantitative Lateral Flow Assay

An Overview for the Nanoparticles‐Based Quantitative Lateral Flow Assay

Rapid point‐of‐care testing methods/devices for meat species identification: A review

A novel electrochemical sandwich-like immunosensor based on carboxyl Ti3C2Tx MXene and rhodamine b/gold/reduced graphene oxide for Listeria monocytogenes

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A novel electrochemical sandwich-like immunosensor based on carboxyl Ti₃C₂T_x MXene and rhodamine b/gold/reduced graphene oxide for Listeria monocytogenes