Label‐free molecular probe based on G‐quadruplex and strand displacement for sensitive and selective detection and naked eye discrimination of exon 2 deletion of AIMP2

Abstract

Exon 2 deletion of aminoacyl tRNA synthetase complex‐interacting multifunctional protein 2 (AIMP2) is a genetic deletion related to various cancers, for instance ovarian and lung cancers. It can be worked as an indicator of cancer for diagnosis of diseases. Here, we developed a label‐free method based on the formation of split G‐quadruplex in the presence of target DNA combined with strand displacement to detect exon 2 deletion of AIMP2 (DE2) sensitively and selectively. This method is easy‐operating and cost‐saving. Moreover, it has observed discrimination of gene deletion from wild‐types by naked eyes. The results demonstrate that this strategy can be further used for the detection of different gene deletions to achieve early diagnosis of diseases and allow better prognosis.

Commentary: Reproducible science, why protocols matter

“…Commentary: Reproducible science, why protocols matter David L. Selwood F I G U R E 1 Detection of exon 2 deletion of AIMP2 based on split a G-quadruplex system, the chemical structure of NMM is shown on the right. Figure taken (Sun et al, 2018) and a second describes a novel fluorescent system for detection of genetic mutations but without using sequencing (Zang, Wang, Zhu, Yao, & Shi, 2018). This system is developed for detection of exon 2 deletion of AIMP2 which is thought to important in some cancers, such as ovarian and lung cancers.…”

“…The DNA and RNA system tools category highlights the increasing importance of this area to therapeutics, two papers on G‐quadruplex systems: one from Ning Sun and co‐workers (Wing‐Leung Wong lab.) describing a propeller‐like molecule as a probe (Sun et al., ) and a second describes a novel fluorescent system for detection of genetic mutations but without using sequencing (Zang, Wang, Zhu, Yao, & Shi, ). This system is developed for detection of exon 2 deletion of AIMP2 which is thought to important in some cancers, such as ovarian and lung cancers.…”

“…Detection of exon 2 deletion of AIMP 2 based on split a G‐quadruplex system, the chemical structure of NMM is shown on the right. Figure taken from Zang et al., with chemical structure of NMM added [Colour figure can be viewed at wileyonlinelibrary.com]…”

Exploration of intramolecular split G-quadruplex and its analytical applications

“…In other words, a G-quadruplex sequence is split into two segments which are linked with consecutive DNA bases, and thus a new G-quadruplex structure may be produced, which is nominated as intramolecular split G-quadruplex (Intra-SG, Scheme 1). There have been a few related reports on utilization of Intra-SG for illumination of logic operations and biosensing (11–13). For example, Yang group constructed various logic gates (OR, INHIBIT, AND and XOR) and combinational logic gates (INHIBIT-OR), using Intra-SG-clamped molecular beacon (MB) (12).…”