Label-free pharmacological profiling based on dynamic mass redistribution for characterization and authentication of hazardous natural products

Song, Hui‐Peng; Wang, Hong; Zhao, Xianda; He, Ling; Zhong, Huailing; Wu, Siqi; Li, Ping; Yang, Hua

doi:10.1016/j.jhazmat.2017.03.025

Cited by 16 publications

(5 citation statements)

References 32 publications

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“…Datura metel L. (Solanaceae) known as baimantuoluo in China, it has been recorded in Chinese Pharmacopoeia ( Chinese Pharmacopoeia Commission, 2015 ). Datura metel L. is prescribed for the treatment of cough, asthma, pain, convulsions, psoriasis, and rheumatism ( Kuang et al, 2011 ; Geng et al, 2015 ; Song et al, 2017 ). Datura metel seeds are the dry seeds of Datura metel L., known as “tianqiezi” or “huqiezi” in China, which also has a long history to be used as traditional Chinese medicine and exhibit significant hypoglycemic ( Murthy et al, 2004 ), antioxidant and antimicrobial ( Bhardwaj et al, 2016 ; Bachheti et al, 2018 ), and analgesic activities ( Wannang et al, 2009 ).…”

Section: Introductionmentioning

confidence: 99%

Metabolites Identification of Bioactive Compounds Daturataturin A, Daturametelin I, N-Trans-Feruloyltyramine, and Cannabisin F From the Seeds of Datura metel in Rats

Liu

Ling

et al. 2018

Front. Pharmacol.

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Datura metel L. is a widely used traditional herbal medicine, and withanolides and amides are the two groups of main bioactive constituents in Datura metel seeds. This study aimed to elucidate the metabolism of four representative bioactive compositions containing daturataturin A (1), daturametelin I (2), N-trans-feruloyltyramine (3), and cannabisin F (4) in rats. After separately oral administration of 20 mg/kg withanolides (1, 2) and amides (3, 4) to rats, a total of 12, 24, and 21 metabolites were detected in the plasma, urine, and fecal samples, respectively. Among them, three hydroxylated metabolites, 1-M3, 2-M2, and 3-M5, were detected in plasma and rat liver microsome incubation system in high abundance. Two metabolites of 1 and 2 were unambiguously identified by comparing with reference standards. Particularly, the methylated metabolite 27α-methoxy-(22R)-22,26-epoxy-27-[(β-D-glucopyranosyl)oxy]ergosta-2,4,6,24-tetraene-1,26-dione (daturametelin L) is a new compound. The withanolides could readily get hydroxylation or methylation metabolism. Meanwhile, the phase II metabolism (glucuronidation or sulfation) was the major reaction for the amides. This is the first study on in vivo metabolism of these active compounds in seeds of Datura metel.

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Section: Introductionmentioning

confidence: 99%

Metabolites Identification of Bioactive Compounds Daturataturin A, Daturametelin I, N-Trans-Feruloyltyramine, and Cannabisin F From the Seeds of Datura metel in Rats

Liu

Ling

et al. 2018

Front. Pharmacol.

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“…In the BTPP method (Biosensor-based Two-phase Pharmacological Profiling) a RWG biosensor is used in the determination of intracellular changes which are induced by the protein relocalization following the stimulation of receptors by the toxicants. The detection principle in this sensor is to convert DMR, caused by the intracellular protein delocalization, into a measurable optical signal [ 117 ].…”

Section: Emerging Biosensor-based Detection Methods Of Natural Producmentioning

confidence: 99%

Emerging biosensors in detection of natural products

Piroozmand

Mohammadipanah

Faridbod

2020

Synthetic and Systems Biotechnology

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Natural products (NPs) are a valuable source in the food, pharmaceutical, agricultural, environmental, and many other industrial sectors. Their beneficial properties along with their potential toxicities make the detection, determination or quantification of NPs essential for their application. The advanced instrumental methods require time-consuming sample preparation and analysis. In contrast, biosensors allow rapid detection of NPs, especially in complex media, and are the preferred choice of detection when speed and high throughput are intended. Here, we review diverse biosensors reported for the detection of NPs. The emerging approaches for improving the efficiency of biosensors, such as microfluidics, nanotechnology, and magnetic beads, are also discussed. The simultaneous use of two detection techniques is suggested as a robust strategy for precise detection of a specific NP with structural complexity in complicated matrices. The parallel detection of a variety of NPs structures or biological activities in a mixture of extract in a single detection phase is among the anticipated future advancements in this field which can be achieved using multisystem biosensors applying multiple flow cells, sensing elements, and detection mechanisms on miniaturized folded chips.

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“…Furthermore, in general, the measurement procedures are not difficult, relatively cheap, and not time-consuming. In the field of natural compound research (for example active compounds in traditional Chinese medicines), novel methods, for example, resonant waveguide grating (RWG) [ 50 , 193 , 194 ] and holographic microscopy can be applied without using any dyes or other labels [ 195 ].…”

Section: Measurement Techniques For Monitoring Cellular Functions Adhesion and Viabilitymentioning

confidence: 99%

Natural Compounds as Target Biomolecules in Cellular Adhesion and Migration: From Biomolecular Stimulation to Label-Free Discovery and Bioactivity-Based Isolation

et al. 2021

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Plants and fungi can be used for medical applications because of their accumulation of special bioactive metabolites. These substances might be beneficial to human health, exerting also anti-inflammatory and anticancer (antiproliferative) effects. We propose that they are mediated by influencing cellular adhesion and migration via various signaling pathways and by directly inactivating key cell adhesion surface receptor sites. The evidence for this proposition is reviewed (by summarizing the natural metabolites and their effects influencing cellular adhesion and migration), along with the classical measuring techniques used to gain such evidence. We systematize existing knowledge concerning the mechanisms of how natural metabolites affect adhesion and movement, and their role in gene expression as well. We conclude by highlighting the possibilities to screen natural compounds faster and more easily by applying new label-free methods, which also enable a far greater degree of quantification than the conventional methods used hitherto. We have systematically classified recent studies regarding the effects of natural compounds on cellular adhesion and movement, characterizing the active substances according to their organismal origin (plants, animals or fungi). Finally, we also summarize the results of recent studies and experiments on SARS-CoV-2 treatments by natural extracts affecting mainly the adhesion and entry of the virus.

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Label-free pharmacological profiling based on dynamic mass redistribution for characterization and authentication of hazardous natural products

Cited by 16 publications

References 32 publications

Metabolites Identification of Bioactive Compounds Daturataturin A, Daturametelin I, N-Trans-Feruloyltyramine, and Cannabisin F From the Seeds of Datura metel in Rats

Metabolites Identification of Bioactive Compounds Daturataturin A, Daturametelin I, N-Trans-Feruloyltyramine, and Cannabisin F From the Seeds of Datura metel in Rats

Emerging biosensors in detection of natural products

Natural Compounds as Target Biomolecules in Cellular Adhesion and Migration: From Biomolecular Stimulation to Label-Free Discovery and Bioactivity-Based Isolation

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