Label-free potentiometric biosensor based on solid-contact for determination of total phenols in honey and propolis

Draghi, Patricia Ferrante; Fernandes, Julio Cesar B.

doi:10.1016/j.talanta.2016.12.002

Cited by 23 publications

(21 citation statements)

References 27 publications

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“…Recent advances in nanomaterial technology have been employed for a variety of biosensors incorporating carbon-based nanomaterials, metallic nanoparticles, or polymers. A solid-contact potentiometric biosensor with two-layer transducer was reported by [ 41 ]. The first layer contains a blend of poly(vinyl) chloride carboxylate (PVC-COOH), graphite and potassium permanganate, and the second one contains a mixture of PVC-COOH and graphite, which was deposited on top of the first one.…”

Section: Biosensors For the Assessment Of Phenol Classesmentioning

confidence: 99%

Biosensors for Antioxidants Detection: Trends and Perspectives

2020

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Herein we review the recent advances in biosensors for antioxidants detection underlying principles particularly emphasizing advantages along with limitations regarding the ability to discriminate between the specific antioxidant or total content. Recent advances in both direct detection of antioxidants, but also on indirect detection, measuring the induced damage on DNA-based biosensors are critically analysed. Additionally, latest developments on (bio)electronic tongues are also presented.

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Section: Biosensors For the Assessment Of Phenol Classesmentioning

confidence: 99%

Biosensors for Antioxidants Detection: Trends and Perspectives

2020

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“…The mechanism of interaction resulted in the elimination of the hydrogen bonds between the amino acids and the MAA monomer units in the polymer. [14] The production of the polymer nanoparticles and the morphology and size of these particles were determined using scanning electron microscopy (SEM). The first polymer particles of the MIP and the non-imprinted polymer (NIP) were prepared by producing a suspension in acetonitrile solvent in Teflon test tubes.…”

Section: Sensor Simulationmentioning

confidence: 99%

“…These images are illustrated in the results section. [2,14] Construction of membrane with MIP modifier Both graphite and gold were applied to construct the body of the microelectrode. However, because of the price of gold, a graphite microelectrode was used in this study.…”

Section: Sensor Simulationmentioning

confidence: 99%

Staphylococcus aureus exotoxin detection using potentiometric nanobiosensor for microbial electrode approach with the effects of pH and temperature

Ahari

Hedayati

Akbari‐adergani

et al. 2017

International Journal of Food Properties

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Considering the ever-increasing population and trends in industrialization, it is difficult to detect the toxins produced in food products using traditional techniques. In this study, a potentiometric nanobiosensor technique using selective patterns for Staphylococcus aureus exotoxin was thoroughly considered. A molecular framework and polymer were produced using methacrylic acid (MAA) monomers, which formed covalent bonds between MAA monomers to produce a white polymer. In addition, hydrogen bonds formed between the amino acids of the exotoxin and the MAA functional groups, which functioned as selective sites for the polymer. To evaluate the effect of pH on the S. aureus exotoxin nanobiosensor, diluted solutions of NaOH and HNO 3 were applied for the upper and lower pH levels, respectively. The effect of temperature was tested using distilled water at fixed temperatures. The results showed that the molecular framework polymer (MFP) in the designed biosensor was able to detect an exotoxin density up to 10-3 M at 68 nm of synthesized molecularly imprinted polymer (MIP) during the first 32 days of the experiment (from a total of 56 days). The potential differences remained constants at an optimum pH range of 5.0-8.5 and at an optimum temperature range of 15°C-25°C. Therefore, we concluded that the pH and temperature can affect the precision of a potentiometric nanobiosensor for detecting S. aureus exotoxin.

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“…The content of phenolic compounds in aqueous samples can be determined by various analytical instrumental methods such as gas chromatography(GC)/mass spectrometric assay [7] spectrophotometric determination [17,18], electro analyticaland potentiometricanalysis [19],high performance liquid chromatographic with various stationary phases as well as, hyper-cross linked polystyrene resin with multiple flow modes, includingisocraticand gradient flow (have been employedwith various detectors such as diode-array [20],mass spectrometry [21]ultraviolet detection [22].…”

Section: Introductionmentioning

confidence: 99%

“…Benzidines were determined in different environmental matrices such as water [15] and river samples [12].Phenolic compounds were determined in various samples, such as environmental [22],food [19], Agricultural [21]seawater,soil, sediments [7,17,18], and wastewater samples [20].…”

Section: Introductionmentioning

confidence: 99%

Liquid Chromatographic and validation study in separation and determination of benzidines and phenols in the main discharge point of wastewater

Altahir¹,

Sultan²,

Mahdi³

et al. 2018

IJCTR

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Benzidines and phenols are the most priority pollutants. Separation and quantitative estimation of priority pollutant benzidines composed of various benzidines BZ, including substituted 3, 3'-dichlorobenzidine DCB and 3, 3'-dimethylbenzidine DMB, and priority pollutant phenols (9 compounds, i.e., phenol, 2-and 4-nitrophenol, 2,4-dimethylphenol, 2-, 2,4-di-, 2,4,6-tri-, Penta-chlorophenol, and 4-chloro-3-methylphenol)was performed using high performance liquid chromatography-ultra violet techniques. Both groups were separated using a C-18 column with a UV detector at a wavelength of 280 nm, and the flow of the mobile phase was isocratic. The mobile phase consisted of 75:25 methanol: water. The column temperature was 50°C, and the flow rate was 1.8 ml/min for the Benzedine's separation. The mobile phase consisted of a 50:50 acetonitrile: phosphate buffer. The optimum pH was 7.1, the flow rate was 0.7 ml/min and the optimum column temperature was 45°C for the phenols separation. The separation parameters were calculated, including the chromatographic parameters such as the capacity factor (k), the number of theoretical plates (N) , the selectivity factor (α), and the resolution factors (Rs).This method was applied to real samples. The water samples that were analyzed were obtained from a petroleum refinery wastewater treatment unit. The results ranged between undetectable levels and 246.9μg/L of the selected benzidines.The results were ranged between undetectable levels and 1865.61 μg/L of the selected phenols.

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Label-free potentiometric biosensor based on solid-contact for determination of total phenols in honey and propolis

Cited by 23 publications

References 27 publications

Biosensors for Antioxidants Detection: Trends and Perspectives

Biosensors for Antioxidants Detection: Trends and Perspectives

Staphylococcus aureus exotoxin detection using potentiometric nanobiosensor for microbial electrode approach with the effects of pH and temperature

Liquid Chromatographic and validation study in separation and determination of benzidines and phenols in the main discharge point of wastewater

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