Label-free protein detection using terahertz time-domain spectroscopy

Han, Xiaohui; Yan, Shihan; Zang, Ziyi; Wei, Dongshan; Cui, Hong‐Liang; Du, Chunlei

doi:10.1364/boe.9.000994

Cited by 26 publications

(13 citation statements)

References 31 publications

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“…Third, THz imaging can be used to detect tumors because the water content of tumor tissues differs greatly from that of normal tissues ( Chen et al., 2011 ; Yamaguchi et al., 2016 ). Forth, the THz wave's high coherence aids in determining the precise refractive index and absorption coefficient of samples ( Han et al., 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Advances of terahertz technology in neuroscience: Current status and a future perspective

Zhang¹,

Li²

2021

iScience

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Summary Terahertz (THz) waves are ranged between microwave and infrared region in the electromagnetic spectrum. THz technology has been demonstrated promising potential for biomedical applications. Exploration of biological effects of THz waves has emerged as a critical new area in life sciences. It is critical to uncover the effects of THz waves on complex biological systems in order to lay out the framework for THz technology development and future applications. Specifically, THz radiation has been shown to affect the nervous system, including the structure of nerve cell membranes, genes expressions, and cytokines level. In this review, we primarily discuss the biological impacts and mechanisms of THz waves on the nervous system at the organisms, cellular, and molecular levels. The future application perspectives of THz technologies in neuroscience are also highlighted and proposed.

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Section: Introductionmentioning

confidence: 99%

Advances of terahertz technology in neuroscience: Current status and a future perspective

Zhang¹,

Li²

2021

iScience

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“…An interesting study was conducted by Han et al using both THz spectroscopy and imaging [174]. These attempted to identify proteins and quantify their content.…”

Section: Current Status Of Thz Spectroscopy For Protein Researchmentioning

confidence: 99%

“…Figure 3.Refractive index of the proteins rn21, rn22, rn28, n42, n43 and n53 for samples with a concentration of 2.0 µg/µL and a quantity of 8 µg. Reprinted from Ref [174]…”

mentioning

confidence: 99%

Terahertz Spectroscopic Analysis in Protein Dynamics: Current Status

et al. 2022

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Proteins play a key role in living organisms. The study of proteins and their dynamics provides information about their functionality, catalysis and potential alterations towards pathological diseases. Several techniques are used for studying protein dynamics, e.g., magnetic resonance, fluorescence imaging techniques, mid-infrared spectroscopy and biochemical assays. Spectroscopic analysis, based on the use of terahertz (THz) radiation with frequencies between 0.1 and 15 THz (3–500 cm−1), was underestimated by the biochemical community. In recent years, however, the potential of THz spectroscopy in the analysis of both simple structures, such as polypeptide molecules, and complex structures, such as protein complexes, has been demonstrated. The THz absorption spectrum provides some information on proteins: for small molecules the THz spectrum is dominated by individual modes related to the presence of hydrogen bonds. For peptides, the spectral information concerns their secondary structure, while for complex proteins such as globular proteins and viral glycoproteins, spectra also provide information on collective modes. In this short review, we discuss the results obtained by THz spectroscopy in the protein dynamics investigations. In particular, we will illustrate advantages and applications of THz spectroscopy, pointing out the complementary information it may provide.

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“…THz imaging decreases both requirements, allowing researchers to see interactions at even smaller molecule sizes with rapid sampling durations. [129][130][131][132][133][134][135][136][137] One such example is depicted in Fig. 6, where THz imaging achieved the same accuracy as fluorine labeling for detecting the presence of streptavidin protein bonding.…”

Section: Protein Interactions and Amino Acidsmentioning

confidence: 99%

Survey of terahertz photonics and biophotonics

et al. 2020

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We review the advances of terahertz (THz) science and technology in biophotonics, including related challenges and solutions. The main impediment to THz spectroscopy and imaging in this field is the high absorption of the THz beam in water. Hence, transmission imaging and spectroscopy of thick wet tissue using THz radiation has generally been quite difficult. However, the absorption of THz waves by water molecules is so strong that increasing the power of the THz source can lead to structural and functional changes in tissues, so solutions must go beyond a larger power output. In terms of resolution, THz imaging is superior to ultrasound but inferior to visible light microscopy. Owing to its unique material analysis capabilities, promising diagnosis applications have been demonstrated through THz imaging and spectroscopy. Unfortunately, many applications are limited by beam penetration depth and resolution. Hence, researchers from a wide variety of scientific and technical fields have been actively improving these features through the development of electronic devices and materials. In addition, groundbreaking optical architecture and materials to reduce beam absorption in the optics of a system and generate focused beams with smaller diameters have been proposed. On the software side, image processing techniques to computationally enhance the resolution and quality of THz imaging have been proposed. Data science and machine learning to automate the diagnosis of defects and diseases through processing THz images and spectroscopy data have been proposed. We have reviewed the applications of THz radiation in biophotonics and research achievements toward advancing these applications. A conclusion with a roadmap toward increasing the footprint of the THz technology in biophotonics is also proposed.

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Label-free protein detection using terahertz time-domain spectroscopy

Cited by 26 publications

References 31 publications

Advances of terahertz technology in neuroscience: Current status and a future perspective

Advances of terahertz technology in neuroscience: Current status and a future perspective

Terahertz Spectroscopic Analysis in Protein Dynamics: Current Status

Survey of terahertz photonics and biophotonics

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