Label-free quantification of host cell protein impurity in recombinant hemoglobin materials
André Henrion,
Cristian-Gabriel Arsene,
Maik Liebl
et al.
Abstract:Quantitative analysis relies on pure-substance primary calibrators with known mass fractions of impurity. Here, label-free quantification (LFQ) is being evaluated as a readily available, reliable method for determining the mass fraction of host cell proteins (HCPs) in bioengineered proteins which are intended for use as protein calibration standards. In this study a purified hemoglobin-A2 (HbA2) protein, obtained through its overexpression in E. coli, was used. Two different materials were produced: natural an… Show more
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