Label-free ratiometric DNA detection using two kinds of interaction-responsive emission dyes

Guo, Yahui; Chen, Qingmin; Qi, Yiting; Xie, Yunfei; Qian, He; Yao, Weirong; Pei, Renjun

doi:10.1016/j.bios.2016.08.041

Cited by 27 publications

(3 citation statements)

References 34 publications

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“…By using two kinds of structure-selective dyes, G4-specific and dsDNA-inserting, our group developed a dual-wavelength ratiometric DNA sensor based on a stem-loop structure. 101 The application of LFBs for cellular sensing or imaging remains a challenge, because factors including membrane permeability, low quantum yield, autofluorescence in the UV band etc. always obstruct the way.…”

Section: Conclusion and Perspectivementioning

confidence: 99%

Label-free DNA-based biosensors using structure-selective light-up dyes

Guo

Hong

et al. 2016

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Label-free biosensors (LFBs) have demonstrated a great potential in cost-effective applications, and most of the DNA-based LFBs are based on the principle of binding-induced structural transformation. This review is a collection of the latest reported studies, which have employed structure-selective nucleic acid dyes for the development of DNA-based LFBs. The collections in this review have been structured based on the selective binding of dyes towards specific DNA conformations, including single-stranded DNA, double-stranded DNA, triplex DNA, i-motifs and G-quadruplexes. The newest studies of employing versatile nucleases, fascinating nanomaterials, logic gates and cascades, DNA junctions and nanostructures have also been collected as examples. It is predicted that the imperative requirement for ultrahigh sensitivity, intelligent analysis, reliable detection, portable and fast assay would force the LFB development in a stimulative and continuous way.

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Section: Conclusion and Perspectivementioning

confidence: 99%

Label-free DNA-based biosensors using structure-selective light-up dyes

Guo

Hong

et al. 2016

Analyst

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“…In addition, label-free biosensors provide further promise without the labeling of electrochemical, colorimetric, or fluorescent reporter elements for measurements. The most important aspects are to preserve the integrity of the identification element and the capability of identifying the target …”

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confidence: 99%

“…The most important aspects are to preserve the integrity of the identification element and the capability of identifying the target. 39 Given the specificity for the detection of biomolecules, the use of aptamer provides an ideal alternative to improve the specificity of DNA walker biosensors. Aptamers, as a type of single-stranded RNA or DNA with a certain sequence, have been discovered and designed with the systematic evolution of ligands by exponential enrichment (SELEX).…”

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confidence: 99%

Smart DNA Machine for Carcinoembryonic Antigen Detection by Exonuclease III-Assisted Target Recycling and DNA Walker Cascade Amplification

Wang

et al. 2017

Anal. Chem.

167

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A synthetic DNA machine performs quasi-mechanical movements in response to external intervention, suggesting the promise of constructing sensitive and specific biosensors. Herein, a smart DNA walker biosensor for label-free detection of carcinoembryonic antigen (CEA) is developed for the first time by a novel cascade amplification strategy of exonuclease (Exo) III-assisted target recycling amplification (ERA) and DNA walker. ERA as the first stage of amplification generates the walker DNA, while the autonomous traveling of the walker DNA on the substrate-modified silica microspheres as the second stage of amplification produces an ultrasensitive fluorescent signal with the help of N-methylmesoporphyrin IX (NMM). The DNA machine as a biosensor could be applied for transducing and quantifying signals from isothermal molecular amplifications, avoiding the complicated reporter elements and thermal cycling. The present biosensor achieves a detection limit of 1.2 pg·mL within a linear range of 10 pg·mL to 100 ng·mL for CEA, along with a favorable specificity. The practical applicability of the biosensor is demonstrated by the detection of CEA in human serum with satisfactory results; thus, it shows great potential in clinical diagnosis.

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