Label-Free Reading of Microarray-Based Immunoassays with Surface Plasmon Resonance Imaging

Kanda, Vishal; Kariuki, James K.; Harrison, David; McDermott, Mark T.

doi:10.1021/ac049318q

Cited by 195 publications

(129 citation statements)

References 39 publications

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“…An alternative to the optical interference-based array is the use of surface plasmon resonance imaging to develop label-free protein arrays. SPR imaging makes it possible to fabricate arrays with controlled surface density of antigens and provides qualitative and quantitative evaluation of antibody binding in a label free format (28 ).…”

Section: Detection Methodsmentioning

confidence: 99%

Antibody Arrays: Technical Considerations and Clinical Applications in Cancer

Sänchez‐Carbayo¹

2006

Clinical Chemistry

120

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Antibody arrays represent one of the high-throughput techniques that are able to detect multiple proteins simultaneously. One of the main advantages of this technology over other proteomic approaches is that the identities of the measured proteins are known or can be readily characterized, allowing a biological interpretation of the results. Features such as lower sample volume and antibody concentration requirements, higher format versatility, and reproducibility support the increasing use of antibody arrays in cancer research. Clinical applications include disease marker discovery for diagnosis, prognosis, and drug response, characterization of signaling and protein pathways, and modifications associated with disease development and progression. This report presents an overview of technical issues of the main antibody array formats and various applications in cancer research. Antibody arrays are high-throughput tools that improve the functional characterization of molecular bases for disease. Furthermore, identification and validation of protein expression patterns, characteristic of cancer progression, and tumor subtypes may intervene and improve tailored therapies in the clinical management of cancer patients.

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Section: Detection Methodsmentioning

confidence: 99%

Antibody Arrays: Technical Considerations and Clinical Applications in Cancer

Sänchez‐Carbayo¹

2006

Clinical Chemistry

120

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“…12,[15][16][17][18][19]26,28,32 Immunoassays, with high selectivity and affinity for the antibody molecules to their corresponding antigens, have been widely exploited for analytical purposes in the field of clinical diagnosis. [36][37][38][39][40][41] Fluorescence spectroscopy has been one of the most widely used readout methods for immunoassay due to its high sensitivity. Immunoassay based on SERS provides a high sensitivity that is comparable to that of fluorescence spectroscopy.…”

Section: -35mentioning

confidence: 99%

SERS Immunoassay Using Microcontact Printing for Application of Sensitive Biosensors

Hong¹,

Jung²

2011

Bulletin of the Korean Chemical Society

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We introduced a promising patterned substrate by using a microcontact printing method that can be used for SERS immunoassays based on antigen-antibody binding. SERS spectrum of the Raman reporter with antibody, which is rhodamine 6G (R6G) adsorbed on colloidal gold nanoparticles, was observed only for the surfaces in which prostate-specific antigen (PSA) is present on the substrate that is attached to an immobilized layer of antibody on the gold nanoparticles layer of the patterned substrate. Raman mapping images clearly showed that the antibodies on the Raman reporter were successfully and selectively conjugated with the antigen on the patterned substrate. This method could be potentially extended to multi-protein detections and ultrasensitive biosensors.

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“…Kanda et al [36] used PDMS microfluidic devices as a surface to pattern the gold and adsorb antigens from the sample -this device had the potential to produce arrays of 64 or more spots. Xinglong et al [37] studied rabbit IgG and its antibody by 2-D detection in different microarray.…”

Section: Surface Plasmon Resonance Detectionmentioning

confidence: 99%

Unconventional detection methods for microfluidic devices

2006

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The direction of modern analytical techniques is to push for lower detection limits, improved selectivity and sensitivity, faster analysis time, higher throughput, and more inexpensive analysis systems with ever-decreasing sample volumes. These very ambitious goals are exacerbated by the need to reduce the overall size of the device and the instrumentation -the quest for functional micrototal analysis systems epitomizes this. Microfluidic devices fabricated in glass, and more recently, in a variety of polymers, brings us a step closer to being able to achieve these stringent goals and to realize the economical fabrication of sophisticated instrumentation. However, this places a significant burden on the detection systems associated with microchip-based analysis systems. There is a need for a universal detector that can efficiently detect sample analytes in real time and with minimal sample manipulation steps, such as lengthy labeling protocols. This review highlights the advances in uncommon or less frequently used detection methods associated with microfluidic devices. As a result, the three most common methods -LIF, electrochemical, and mass spectrometric techniques -are omitted in order to focus on the more esoteric detection methods reported in the literature over the last 2 years.

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Label-Free Reading of Microarray-Based Immunoassays with Surface Plasmon Resonance Imaging

Cited by 195 publications

References 39 publications

Antibody Arrays: Technical Considerations and Clinical Applications in Cancer

Antibody Arrays: Technical Considerations and Clinical Applications in Cancer

SERS Immunoassay Using Microcontact Printing for Application of Sensitive Biosensors

Unconventional detection methods for microfluidic devices

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