Labeling and sequencing nucleic acid modifications using bio-orthogonal tools

Abstract

The bio-orthogonal reaction is a type of reaction that can occur within a cell without interfering with the active components of the cell. Bio-orthogonal reaction techniques have been used to...

Qualitative and Quantitative Analytical Techniques of Nucleic Acid Modification Based on Mass Spectrometry for Biomarker Discovery

“…It is expected that the discovery of new modifications will be achieved through higher-sensitivity detection and better untargeted tandem mass spectrometry data processing methods. Meanwhile, quantification methods based on chemical labeling can be extended to the development of sequencing methods [ 49 , 163 ], obtaining information about the modifications to the genome and transcriptome, and investigating the mechanisms by which modifications affect biological functions. With more biological models validated, more analytical methods will be applied to life processes and diseases that are currently difficult to explore.…”