Laboratory and field growth studies of commercial strains ofEucheuma denticulatum andKappaphycus alvarezii in the Philippines

Dawes, Clinton J.; Lluisma, Arturo O.; Trono, Gavino C.

doi:10.1007/bf02185899

Cited by 103 publications

(52 citation statements)

References 5 publications

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“…In some cases, however, this performance was higher (Ramus & van der Meer 1983, Dawes et al 1994, Guimarães 2000. In the present study, green and red strains of Gracilaria cornea presented similar growth rates in all tested conditions, suggesting the potential of the green strain to be tested in mariculture experiments.…”

Section: Discussionmentioning

confidence: 89%

Growth of red and green strains of the tropical agarophyte Gracilaria cornea J. Agardh (Gracilariales, Rhodophyta) in laboratory

2006

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-(Growth of red and green strains of the tropical agarophyte Gracilaria cornea J. Agardh (Gracilariales, Rhodophyta) in laboratory). Gracilaria cornea J. Agardh is an important agarophyte occurring in the western Atlantic Ocean. Green colour individuals of G. cornea were found in a natural population, growing next to red individuals, which were more common. Due to the importance of colour strains in genetic and intraspecific variability studies, this work aimed to characterize the red and green strains evaluating different nutritional and light conditions. Red and green gametophytes were cultivated at 14:10 light: dark cycle, with alternating aeration periods of 30 min. Two different enriched solutions were tested: von Stosch (VSS) at concentrations reduced to 12.5% and 25%; and Provasoli (PES) at concentrations reduced to 25% and 50%, and 100%. Red and green gametophytes were cultivated at the irradiance of 45 µmol photons m -2 s -1 . In another experiment utilizing PES 100%, two sources of light (Osram 40 W daylight fluorescent tubes and Sylvania Designer 3,500 tubes) were tested at irradiances of 90 and 180 µmol photons m -2 s -1. Growth rates (GR) were evaluated for five weeks. Gametophytes developed few branches and reproductive structures were not induced. Differences were not observed between GR of red and green strains in the conditions tested. GR were higher in VSS 12.5% (8.4% day -1 ) than in 25% (7.1% day -1 ), suggesting an adaptation of the species to low nutrient concentrations. GR were higher at 180 (9.0% day -1 ) than at 90 µmol photons m -2 s -1 (6.3% day -1 ). These results suggest that G. cornea should be cultivated in laboratory at high irradiances and low nutrient concentrations. These data will be useful in future genetic and physiological studies of the species.Key words -agarophyte, colour strain, Gracilaria cornea, growth, light RESUMO -(Crescimento de linhagens de coloração verde e vermelha da agarófita tropical Gracilaria cornea J. Agardh (Gracilariales, Rhodophyta) em laboratório). Gracilaria cornea J. Agardh é uma importante agarófita que ocorre no Oeste do Oceano Atlântico. Indivíduos de coloração verde de G. cornea foram encontrados em uma população natural, próximos a indivíduos de coloração vermelha, mais comuns. Devido à importância de linhagens cromáticas em estudos de genética e variabilidade intraespecífica, o objetivo deste trabalho foi caracterizar as linhagens verde e vermelha avaliando diferentes condições de nutrintes e luz. Gametófitos verdes e vermelhos foram cultivados em fotoperíodo de 14 horas (14L:10E), com períodos alternados de aeração de 30 minutos. Duas soluções de água do mar enriquecida foram testadas: von Stosch (VSS) nas concentrações reduzidas a 12,5% e 25%; e Provasoli (PES) nas concentrações reduzidas de 25% e 50%, e 100%. A irradiância foi de 45 µmol fótons m -2 s -1 . Num outro experimento, utilizando-se PES 100%, dois tipos de fontes luminosas (lâmpadas fluorescentes Osram 40 W luz do dia e Sylvania Designer 3.500) foram testadas em duas irra...

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Section: Discussionmentioning

confidence: 89%

Growth of red and green strains of the tropical agarophyte Gracilaria cornea J. Agardh (Gracilariales, Rhodophyta) in laboratory

2006

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“…Earlier reports were made by Fries (1984) in Fucus and Dawes et al (1994) in Kappaphycus on the use of seaweed extract for tissue culture. However, the use of AMPEP with known N-P and other macronutrients composition is used for the first time in tissue culture technique in the present study to germinate shoots and regenerate microplantlets.…”

Section: Discussionmentioning

confidence: 96%

“…Successful micropropagation of Kappaphycus alvarezii and Eucheuma denticulatum has been reported by Dawes and Koch (1991), Dawes et al (1993Dawes et al ( , 1994, Hurtado and Cheney (2003) and Hurtado and Biter (2007) using different culture media. Reddy et al (2003) reported the somatic embryogenesis and regeneration of embryos from pigmented callus of K. alvarezii as a potential means for clonal production of seed stock for commercial farming.…”

Section: Introductionmentioning

confidence: 89%

Use of Acadian marine plant extract powder from Ascophyllum nodosum in tissue culture of Kappaphycus varieties

et al. 2008

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Three varieties of Kappaphycus alvarezii (Kapilaran, KAP), Tambalang purple (PUR), Adik-adik (AA), and one variety of Kappaphycus striatum var. sacol (green sacol (GS) were used to determine the efficiency of Acadian marine plant extract powder (AMPEP) as a culture medium at different concentrations, for the regeneration of young plants of Kappaphycus varieties, using tissue culture techniques for the production of seed stock for nursery and outplanting purposes for the commercial cultivation of carrageenophytes. A shorter duration for shoot formation was observed when the explant was treated with AMPEP+Plant Growth Regulator (PGR=PAA+zeatin at 1 mg L −1 ) compared to AMPEP when used singly. However, four explants responded differently to the number of days required for shoot formation. The KAP variety took 46 days to form shoots at 3-4 mg L −1 AMPEP+PGR; while PUR required 21 days at 3-5 mg L −1 AMPEP and 3-4 mg L −1 AMPEP+PGR. AA required 17 days at 3-5 mg L −1 AMPEP and AMPEP+PGR; and GS 25 days at 1 mg L −1 AMPEP+ PGR. It was observed that among the four explants used, PUR and AA initiated shoot formation with the use of AMPEP only at higher concentrations (3-5 mg L −1 ) after a shorter period. Only PUR responded positively to ESS/2 for shoot initiation. The use of AMPEP alone and/or in combination with PGR as a culture medium in the propagation of microplantlets using tissue culture technique is highly encouraging.

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“…Estes resultados são semelhantes aos verificados para a maioria das variantes cromáticas de Rhodophyta, que apresentou um menor desempenho, quando comparadas aos tipos selvagens (van der Meer 1990). No entanto, em alguns casos, este desempenho foi semelhante ou até superior (Ramus & van der Meer 1983, Dawes et al 1994 Figura 2. Taxas de crescimento (Dia -1 , média + desvio padrão) calculadas a partir das medidas de comprimento de espécimes de coloração vermelha (vm) e verde clara (vc) de Gracilaria sp.…”

Section: Discussionunclassified

Crescimento in vitro de linhagens de coloração vermelha e verde clara de Gracilaria birdiae (Gracilariales, Rhodophyta) em dois meios de cultura: análise de diferentes estádios reprodutivos

Ursi¹,

Plastino²

2001

Rev. bras. Bot.

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Laboratory and field growth studies of commercial strains ofEucheuma denticulatum andKappaphycus alvarezii in the Philippines

Cited by 103 publications

References 5 publications

Growth of red and green strains of the tropical agarophyte Gracilaria cornea J. Agardh (Gracilariales, Rhodophyta) in laboratory

Growth of red and green strains of the tropical agarophyte Gracilaria cornea J. Agardh (Gracilariales, Rhodophyta) in laboratory

Use of Acadian marine plant extract powder from Ascophyllum nodosum in tissue culture of Kappaphycus varieties

Crescimento in vitro de linhagens de coloração vermelha e verde clara de Gracilaria birdiae (Gracilariales, Rhodophyta) em dois meios de cultura: análise de diferentes estádios reprodutivos

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