Laboratory and Semi - Field Evaluation and Effect of Clove Essential - Oil against Two - Spotted Spider - Mite Tetranychus urticae, Koch. (Acari:Tetranychidae)

Abstract

The Effects of clove, Syzygium aromaticum essential-oil against spider-mite Tetranychus urticae, Koch was estimated in lab and semi-field conditions. Treated adult-females conducted by different concentrations of clove essential oil (eugenol) concentrations using spraying method for 1, 3 and 7 days. Mortality was measured upon treatment with three concentrations of 0.25, 0.50 and 1.0% V/V. Female mortality increasing with increasing concentrations and LC50 value was calculated. Moreover, all the tested concentrations of the essential oil were significant. The effect of LC50 of eugenol on the neuronal-enzymes acetylcholine-esterase "AchE" and alkalinephosphatase "ALP" of spider-mites were investigated on 24 hours and 3 days post-treatment. The obtained data resulted the activity of "AchE" and "ALP" showed decreasing after treatment on 24 hours by clove-oil while, "ALP" showed increasing on 3-days post-treatment. The evaluation of clove essential oil effect in semi-field condition on "T. urticae", results indicated that it gave satisfying results whereas 92 % population reduction respectively. Finally, we can conclude that clove essential oil was achieved good results to control this spider mites.

Chemical Characterization of Clove, Basil and Peppermint Essential Oils; Evaluating Their Toxicity on the Development Stages of Two-Spotted Spider Mites Grown on Cucumber Leaves

“…Clove oil with its main component eugenol showed comparable effects to abamectin when comparing basil and peppermint EOs. In this regard, Mahmoud and Kassem [56] found that clove essential oil has a considerable effect against T. urticae, showing that mortality was high at 24 h and three days post treatment. No effect on mortality percentage was observed after seven days post-treatment.…”