Laboratory Animal Models for Brucellosis Research

Silva, Teane M. A.; Costa, Érica Azevedo; Paixão, Tatiane Alves da; Tsolis, Renée M.; Santos, Renato L.

doi:10.1155/2011/518323

Cited by 90 publications

(98 citation statements)

References 80 publications

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“…It was also reported that BALB/c pregnant female mice infected with 10 6 cfu of B. abortus virulent strain 2308 developed a moderate multifocal necrotic placentitis associated with severe neutrophilic infiltrate and intralesional bacteria in trophoblastic cells (Silva et al, 2011).…”

Section: Discussionmentioning

confidence: 88%

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CLINICAL AND REPRODUCTIVE PATHOLOGICAL CHANGES ASSOCIATED WITH <i>BRUCELLA MELITENSIS</i> AND ITS LIPOPOLYSACCHARIDES IN FEMALE MICE VIA ORAL INOCULATION

Abdullah

Adamu

Hazirah

et al. 2013

American Journal of Animal and Veterinary Sciences

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Brucella melitensis (B. melitensis) are Gram-negative, aerobic, facultative intracellular bacteria that cause brucellosis that usually leads to abortion in sheep and goats. Three groups of equal number of 24 healthy female mice were used as animal models. They were orally inoculated with 0.4 mL of phosphate Buffered Saline (PBSControl group), 0.4 mL of 10 9 cfu of B. melitensis and 0.4 mL of Lipopolysaccharides (LPS) extracted from 10 9 cfu of B. melitensis (both as treatment groups). Clinical signs exhibited by the mice were observed for 10 days, after which the survived mice were euthanized by cervical dislocation. Following that, post mortem was conducted and histopathological study of the reproductive organs was carried out. B. melitensis group showed mild clinical signs compared to LPS group which showed normal behaviours except for mild ruffled fur, 14 and 34 h post-inoculation, respectively. The control group (PBS) showed normal behaviours. Histopathology results revealed that both B. melitensis and LPS groups showed mild to moderate infiltration of inflammatory cells in the reproductive organs, along with normal to mild findings of necrosis. Mild to moderate haemorrhage were found in the mice of B. melitensis group, while LPS group showed normal to mild haemorrhage and moderate to severe congestion of the ovary. The study proved that mice infected orally with B. melitensis developed mild clinical signs whereas mice orally inoculated by its LPS showed normal behavior except for the mild ruffled fur. Moreover, both groups of mice inoculated with B. melitensis immunogens developed pathological changes in the reproductive organs. The LPS of B. melitensis could be a potential candidate for the development of vaccines.

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Section: Discussionmentioning

confidence: 88%

“…that affects humans as well as domestic and wild animals, with reproductive disorders as the main problem in domestic animals (Megid et al, 2010;Silva et al, 2011). The validly published species of Brucella are B. melitensis, B.…”

Section: Introductionmentioning

confidence: 99%

CLINICAL AND REPRODUCTIVE PATHOLOGICAL CHANGES ASSOCIATED WITH <i>BRUCELLA MELITENSIS</i> AND ITS LIPOPOLYSACCHARIDES IN FEMALE MICE VIA ORAL INOCULATION

Abdullah

Adamu

Hazirah

et al. 2013

American Journal of Animal and Veterinary Sciences

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“…For this, clinical data and mostly laboratory in vivo models, in which different infection stages can be monitored, are essential. A widely used Brucella in vivo model is mouse, which largely recapitulates natural animal brucellosis (Silva et al 2011). Several Bartonella species also have a small animal model reflecting infection either (1) in the reservoir host (Fig.…”

Section: Lessons Learned From In Vivo Studiesmentioning

confidence: 99%

Bartonella and Brucella--Weapons and Strategies for Stealth Attack

Ben-Tekaya

Gorvel

Dehio

2013

Cold Spring Harbor Perspectives in Medicine

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“…. It has a devastating impacts on public health, infecting around 500,000 new cases each year globally (Silva et al, 2011). The incidence of the disease is reported to be 2-8/ 1,000,000 in a global incidence report; however, according to World Organization for Animal Health (OIE) the incidence of brucellosis is 3.8 per million in the country (Saeed et al, 2014).…”

Section: …………………………………………………………………………………………………… Introduction:-mentioning

confidence: 99%

Brucellosis Awareness and Risk Factors Among Population of Paghman District of Kabul Province - Afghanistan.

popalzai¹

2017

IJAR

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Zoonoses are a major health problem in Afghanistan but there is very limited data to describe the effectiveness of awareness and management of these disease particularly brucellosis. A cross-sectional study was conducted which focused on Brucellosis awareness among the residents of the targeted area specifically the people holding livestock. The study included both men and women engaged with livestock interventions and people showed the clinical picture of Brucellosis. The study population were of Paghman district in Kabul province of Afghanistan that is a rural area where majority of the residents rely on animals" rearing for their daily income and food. Population being engaged with livestock interventions was identified seeking the help of local veterinary practitioners and VFUs (Veterinary Field Units) in Paghman district. A random selection of households were applied once they were identifies as inclusive segments of this study. A total of 400 people were randomly selected to be interviewed. A structured questionnaire during face to face interviews was used for the study within the context of the study specifically focusing on Brucellosis signs and symptoms. A total of 244 male (61%) and 156 (39%) female were interviewed with outmost efforts to include equal representation and convincing community for females in the study. A major problem was in the equal selection of participants from male and female was cultural constraints at the community level given that women are not allowed to be interviewed. Substantial majorities (77%) of respondents were able to correctly answer to the knowledge of Brucellosis but specific knowledge of signs and symptoms was less consistent. Gender wise respondents" knowledge of brucellosis was higher among men (75%) than women (25%). Brucellosis transmission knowledge of respondents knowing the main routes of agent was found considerably significant since the awareness programs are running at country level. More than half of the both participants both men and women were able to describe the main routes of brucellosis. More than 90% of respondents strongly agreed with the statement that "brucellosis occurrence can be reduced through improving public awareness". However, only between 21.1% and 38.3% of respondents either agreed or strongly agreed that people usually do not follow professionals" advise and changing behaviors to avoid becoming infected with a zoonotic disease. The findings of this

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Laboratory Animal Models for Brucellosis Research

Cited by 90 publications

References 80 publications

CLINICAL AND REPRODUCTIVE PATHOLOGICAL CHANGES ASSOCIATED WITH <i>BRUCELLA MELITENSIS</i> AND ITS LIPOPOLYSACCHARIDES IN FEMALE MICE VIA ORAL INOCULATION

CLINICAL AND REPRODUCTIVE PATHOLOGICAL CHANGES ASSOCIATED WITH <i>BRUCELLA MELITENSIS</i> AND ITS LIPOPOLYSACCHARIDES IN FEMALE MICE VIA ORAL INOCULATION

Bartonella and Brucella--Weapons and Strategies for Stealth Attack

Brucellosis Awareness and Risk Factors Among Population of Paghman District of Kabul Province - Afghanistan.

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