Tetranychus urticae Koch is the most harmful pest to many plants in the globe. This previously completed work may offer fresh perspectives on the use of Thuja orientalis, a plant extract, as environmentally beneficial acaricide. The effectiveness of various quantities of plant extract from T. orientalis against adult females of the two-spotted spider mite T. urticae, with LC50 values of 8.35, 6.310, and 4.48 after one day, two days, and three days, respectively. In addition to the abamectin (vertimec 1.8 EC) acaricide's acaricidal efficacy at various concentrations, where the LC50 values were 1.02, 2.74, and 1.65 after one day, two days, and three days, respectively. Furthermore, the adult fecundity, repellency, and hatchability of T. orientalis plant extract and vertimec varied substantially with concentration. The findings of this study suggested that the plant extract of T. orientalis might be used as a bio acaricide compared with vertimec to control spider mites.