Laboratory processing of cerebrospinal fluid specimens

Jm, Andrews; Gb, Schumann

doi:10.1002/dc.2840060215

Cited by 5 publications

(2 citation statements)

References 23 publications

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“…13,14 Other reports suggested that only a limited percentage of body fluids with low cell counts are suitable for flow cytometry. 12,15 In that study, 31 CSF specimens were analyzed, and in 15 cases there were inadequate cell numbers for analysis, indicating limited value of flow cytometry in CSF analysis due to its usual hypocellularity. Since hypocellularity is the usual problem in the analysis of CSF cells, in a previous study 25 ml of CSF were obtained for flow cytometry of CSF cells.…”

Section: Csf Cells In Cns Diseasesmentioning

confidence: 99%

Analysis of Cerebrospinal Fluid Cells by Flow Cytometry and Immunocytochemistry in Inflammatory Central Nervous System Diseases: Comparison of Low‐ and High‐Density Cell Surface Antigen Expression

Windhagen

Maniak

Heidenreich

1999

Diagnostic Cytopathology

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The examination of cerebrospinal fluid (CSF) continues to play an important role in the diagnosis of inflammatory diseases of the central nervous system (CNS). Immunocytochemistry and flow cytometry are the most commonly used methods for analysis of surface markers on CSF cells. We here compared these methods in the examination of CSF cells from a total of 68 patients with acute and chronic inflammatory CNS diseases. Expression of costimulatory molecules CD80 (B7‐1) and CD86 (B7‐2) as activation markers that are present at low density on the cell surface was analyzed in comparison to CD22 (B‐cells) and CD4 (T‐cell subset), that show high staining intensities. For CD22 and CD4, the results obtained with both methods were similar and reliable. Using flow cytometry, CD80 expression was detected in 6% of CSF cells in patients with chronic inflammatory CNS disease, as compared to 2% using immunocytochemistry, where the reliability of the data was found to be higher. We conclude that for examination of low‐density surface markers on CSF cells, particularly with low cell counts, immunocytochemistry may be more reliable. Diagn. Cytopathol. 1999;21:313–318. © 1999 Wiley‐Liss, Inc.

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Section: Csf Cells In Cns Diseasesmentioning

confidence: 99%

Analysis of Cerebrospinal Fluid Cells by Flow Cytometry and Immunocytochemistry in Inflammatory Central Nervous System Diseases: Comparison of Low‐ and High‐Density Cell Surface Antigen Expression

Windhagen

Maniak

Heidenreich

1999

Diagnostic Cytopathology

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“…Although flow cytometry has been successfully used in the analysis of surface antigen expression on CSF cells, [12][13][14] it was previously suggested that this method may not be suitable in the evaluation of samples with low cellularity. 12,15 We wanted to compare both flow cytometry and immunocytochemistry in the examination of different surface antigens on CSF cells. For immunocytochemistry, a sensitive peroxidase staining procedure was used to detect cell surface antigens on CSF cytospins.…”

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confidence: 99%