Laboratory versus wild populations: the importance of population origin in aquatic ecotoxicology

Romero-Blanco, Alberto; Alonso, Álvaro

doi:10.1007/s11356-021-17370-0

Cited by 14 publications

(7 citation statements)

References 67 publications

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“…Reproducibility in data outcomes has been reported for regulatory testing for some time (Berry, 2014) and is increasingly recognised as a problem in research, most notably in areas of research including neurobiology, behaviour and cognition studies (Gerlai, 2018), epigenetics (Sugden et al ., 2020), population modelling in ecotoxicology (Wang, 2018) and comparisons between laboratory fish models and wild populations (Romero‐Blanco & Alonso, 2022). Reproducibility is known to be affected by differences in housing and husbandry of experimental animals (Lieggi et al ., 2020).…”

Section: Introductionmentioning

confidence: 99%

Beyond compliance: harmonising research and husbandry practices to improve experimental reproducibility using fish models

Paull,

Lee,

Tyler

2023

Biological Reviews

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Reproducibility in animal research is impacted by the environment, by husbandry practices in the laboratory and by the animals' provenance. These factors, however, are often not adequately considered by researchers. A disconnect between researchers and animal care staff can result in inappropriate housing and husbandry decisions for scientific studies with those animals. This is especially the case for the research in neuro‐behaviour, epigenetics, and the impact of climate change, as heritable phenotypic, behavioural or physiological changes are known to result from the animals' environmental housing, husbandry, provenance and prior experience. This can lead to greater variation (even major differences) in data outcomes among studies, driving scientific uncertainties. Herein, we illustrate some of the endpoints measured in fish studies known to be intrinsically linked to the environment and husbandry conditions and assess the significance of housing and husbandry practice decisions for research adopting these endpoints for different fish species. We highlight the different priorities and challenges faced by researchers and animal care staff and how harmonising their activities and building greater understanding of how husbandry practices affect the fish will improve reproducibility in research outcomes. We furthermore illustrate how improving engagement between stakeholders, including regulatory bodies, can better underpin fish husbandry decisions and where researchers could help to drive best husbandry practices through their own research with fish models.

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Section: Introductionmentioning

confidence: 99%

Beyond compliance: harmonising research and husbandry practices to improve experimental reproducibility using fish models

Paull,

Lee,

Tyler

2023

Biological Reviews

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“…To our knowledge, similar comparative ecotoxicological studies examining sensitivity differences between contaminant-exposed wild and laboratory embryo–larval FHM are not available in the peer-reviewed literature. However, a small number of studies utilizing other model species report similar differences in sensitivity between wild and laboratory populations, though the findings are species and toxicant dependent and concentrate on effects during adult life stages . For example, a study examining the comparative sensitivities wild and laboratory Caenorhabditis elegans found that all four wild strains tested were significantly more sensitivity to copper exposure relative to lab-cultured organisms .…”

Section: Discussionmentioning

confidence: 67%

“…However, a small number of studies utilizing other model species report similar differences in sensitivity between wild and laboratory populations, though the findings are species and toxicant dependent and concentrate on effects during adult life stages. 63 For example, a study examining the comparative sensitivities wild and laboratory Caenorhabditis elegans found that all four wild strains tested were significantly more sensitivity to copper exposure relative to lab-cultured organisms. 64 Similarly, wild male bank voles demonstrated a higher relative sensitivity to cadmium exposure when compared with their laboratory-bred counterparts, with potentially important implications for small burrowing mammalian receptors living at contaminated sites, which tend to be among the most important drivers of ecological risk.…”

Section: ■ Discussionmentioning

confidence: 99%

Comparative Effects of Embryonic Metformin Exposure on Wild and Laboratory-Spawned Fathead Minnow (Pimephales promelas) Populations

Nielsen

DeCamp

Birgisson

et al. 2022

Environ. Sci. Technol.

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Metformin is routinely detected in aquatic ecosystems because of its widespread use as a treatment for Type 2 diabetes. Laboratory studies have shown that exposure to environmentally relevant concentrations of metformin can alter metabolic pathways and impact the growth of early life stage (ELS) fish; however, it is unknown whether these effects occur in wild populations. Herein, we evaluate whether findings from laboratory studies are representative and describe the relative sensitivities of both populations. Duplicate exposures (0, 5, or 50 μg/L metformin) were conducted using wild- and lab-spawned fathead minnow (Pimephales promelas) embryos. Apart from the water source, exposure conditions remained constant. Wild embryos were exposed to previously dosed lake water to account for changes in bioavailability, while reconstituted freshwater was used for the laboratory study. Developmental metformin exposure differentially impacted the growth and morphology of both cohorts, with energy dyshomeostasis and visual effects indicated. The fitness of wild-spawned larvae was impacted to a greater extent relative to lab-spawned fish. Moreover, baseline data reveal important morphological differences between wild- and lab-spawned ELS fatheads that may diminish representativeness of lab studies. Findings also confirm the bioavailability of metformin in naturally occurring systems and suggest current exposure scenarios may be sufficient to negatively impact developing fish.

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“…In the same region, D. magna populations isolated from ponds located in agricultural areas presented specific pesticide resistance in line with local management practices (either chlorpyrifos or deltamethrin; Almeida et al, 2021). Romero‐Blanco and Alonso (2022) also noted from database and literature review that the sensitivity of aquatic species to contaminants depended strongly on the origin of populations, with either wild or laboratory‐reared populations being the most tolerant, depending on the chemical. However, the persistence of the most tolerant populations may come not only with a reduction of genetic diversity but also with fitness or physiological costs (Coustau et al, 2000; Jansen et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Variation of Tolerance to Isothiazolinones Among Daphnia pulex Clones

Wagner-Deyriès

Varignier

Revel

et al. 2023

Enviro Toxic and Chemistry

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Isothiazolinones are a family of broad‐spectrum biocides widely used in industry and consumer products. Chloro‐ and methyl‐isothiazolinones (CMIT and MIT) are documented as strong irritants, yet they are still used in a wide variety of applications, including cosmetics, cleansers, hygienic products, and various industrial applications. The subsequent substantial release of these molecules from urban sources into freshwater environments, and their potential impacts on aquatic species, have nevertheless received little attention so far, with few studies reporting on the toxicity of either CMIT or MIT to nontarget organisms. The present study addresses this current knowledge gap by evaluating the acute toxicity to Daphnia pulex (Cladocera) of CMIT/MIT (3:1) and MIT, the two formulations most commonly used by manufacturers. In addition, genetic diversity is known to be a major component of variability in phenotypic responses, although it is largely overlooked in typical toxicity tests. Thus the potential range of responses inherent to genetic diversity is rarely considered. Therefore, to account for intraspecific variations in sensitivity, our design involved eight clonal lines of D. pulex stemming from distinct natural populations or commercial strains. Clones exhibited strong variation in their responses, with median lethal concentration (LC50) values ranging from 0.10 to 1.84 mg/L for the mixture CMIT/MIT, and from 0.68 to 2.84 mg/L for MIT alone. These intraspecific ranges of LC50 values challenge the use of single clones of daphnids in standard ecotoxicological tests and the predictions based on their results. The present study brings new evidence that assessing ecological risk of chemicals while ignoring genotype diversity is neither ecologically relevant, nor a representative evaluation of the diversity of potential adverse outcomes. Environ Toxicol Chem 2023;42:805–814. © 2023 SETAC

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Laboratory versus wild populations: the importance of population origin in aquatic ecotoxicology

Cited by 14 publications

References 67 publications

Beyond compliance: harmonising research and husbandry practices to improve experimental reproducibility using fish models

Beyond compliance: harmonising research and husbandry practices to improve experimental reproducibility using fish models

Comparative Effects of Embryonic Metformin Exposure on Wild and Laboratory-Spawned Fathead Minnow (Pimephales promelas) Populations

Variation of Tolerance to Isothiazolinones Among Daphnia pulex Clones

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