Lactate dehydrogenase: sequence and analysis of its expression during the life cycle of Schistosoma mansoni

Guerra‐Sá, Renata; Franco, Glória Regina; Pena, Sérgio D.J.; Rodrigues,

doi:10.1590/s0074-02761998000700035

Cited by 8 publications

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“…One of lowly expressed genes (EWB00_010161) in SD-F is lactate dehydrogenase (LDH), encoding a terminal glycolytic enzyme that catalyzes the interconversion of pyruvate and lactate in the presence of the nicotinamide adenine dinucleotide coenzyme. Schistosome parasitic stages depend mainly on anaerobic energy metabolism and glycolytic enzymes are essential for the survival of parasites [41,42]. Four genes (EWB00_005036, EWB00_003584, EWB00_003582 and EWB00_009712) have no functional domains or unknown domains.…”

Section: Discussionmentioning

confidence: 99%

Oncomelania hupensis retains its ability to transmit Schistosoma japonicum 13 years after migration from permissive to non-permissive areas

et al. 2020

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Background: The East Route Project (ERP) of the South-to-North Water Diversion Project (SNWDP) stretches across schistosomiasis endemic and non-endemic areas in China, which may lead to the dispersal of Oncomelania hupensis, the intermediate host of Schistosoma japonicum, from permissive areas along the Yangtze River Basin to non-permissive areas in northern China. A previous survey demonstrated that O. hupensis could survive and breed for 13 years (12 generations) after being transferred to a non-permissive area, and could be infected by S. japonicum. However, it is not clear if the migrated snails will change their ability to transmit S. japonicum. Methods: We infected mice with the cercariae released from the infected transferred snails bred in Jining city of Shandong Province (non-permissive areas) for 13 years. The mice in the control group were infected with cercariae derived from the snails collected in their original habitat (Jiangdu county of Jiangsu Province, permissive areas). Then, we explored the pathogenicity to mice including worm burden, liver egg count and pathology. Additionally, the gene expression profiles of the adult male and female worms recovered from the infected mice were analyzed by RNA sequencing. Results: The worm burden, liver egg count and pathology of the mice infected with cercariae released from transferred snails bred in non-permissive areas for 13 years showed no significant differences, when compared with the control cercariae. Slight changes occurred at the transcription level between adult male and female worms recovered from mice infected with cercariae derived from snails bred in permissive and non-permissive areas. Only fourteen genes were significantly differentially expressed in the comparison of adult female worms, and no significantly differentially expressed gene was found in the comparison of adult male worms. Conclusions: Our findings strongly suggest that transferred snails did not change their schistosomiasis transmission ability and the worms derived from them retained the original pathogenicity, even after migrating from permissive to non-permissive areas for 13 years. Therefore, a long-term surveillance system of snails along the SNWDP is urgently needed to prevent the diffusion of O. hupensis and reduce the risk of transmission of schistosomiasis.

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Section: Discussionmentioning

confidence: 99%

Oncomelania hupensis retains its ability to transmit Schistosoma japonicum 13 years after migration from permissive to non-permissive areas

et al. 2020

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“…Guerra-Sa et al . (1998) reported the putative preferential transcription for LDH in S. mansoni males compared with females; further, they showed that LDH was expressed in S. mansoni eggs, miracidia, cercariae and schistosomula, with higher levels in adults (Guerra-Sa et al . 1998).…”

Section: Discussionmentioning

confidence: 99%

“…LDH is an ubiquitous enzyme (Guerra-Sa et al 1998; Lu et al 2006; Wiwanitkit, 2007) that catalyses the conversion of pyruvate to lactate with the concomitant interconversion of NADH and NAD as the terminal reaction in glycolysis. Guerra-Sa et al (1998) reported the putative preferential transcription for LDH in S. mansoni males compared with females; further, they showed that LDH was expressed in S. mansoni eggs, miracidia, cercariae and schistosomula, with higher levels in adults (Guerra-Sa et al 1998). Similarily, the current study showed a higher level of transcription representing Contig 8557 (LDH) in adult S. japonicum , highest levels being in males compared with females (Tables 1, 3 and 4).…”

Section: Discussionmentioning

confidence: 99%

Comparative real-time PCR and enzyme analysis of selected gender-associated molecules inSchistosoma japonicum

2008

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SUMMARYSchistosomes are complex parasitic helminths with discrete life-cycle stages, adapted for survival in their mammalian and snail hosts and the external aquatic environment. Recently, we described the fabrication and use of a microarray to investigate gender-specific transcription in Schistosoma japonicum. To address transcriptional differences, 8 gender-associated gene transcripts identified previously by the microarray analysis were selected for further study. First, differential transcription patterns were investigated in 4 developmental stages using real-time PCR. Subsequently, we undertook functional analysis of a subset of 4 transcripts encoding metabolic enzymes, so as to correlate gender-associated transcript levels with enzyme activity in protein extracts from adult worms. The 8 characterized molecules serve as a basis for further investigation of differential gene expression during the schistosome life-cycle and for studying the sexual dimorphism of adult worms. Continual refinement and annotation of the microarray used in the current study should support future work on these aspects.

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Lactate dehydrogenase and malate dehydrogenase: Potential antiparasitic targets for drug development studies

Kayamba

Faya

Pooe

et al. 2021

Bioorganic & Medicinal Chemistry

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Lactate dehydrogenase: sequence and analysis of its expression during the life cycle of Schistosoma mansoni

Cited by 8 publications

References 0 publications

Oncomelania hupensis retains its ability to transmit Schistosoma japonicum 13 years after migration from permissive to non-permissive areas

Oncomelania hupensis retains its ability to transmit Schistosoma japonicum 13 years after migration from permissive to non-permissive areas

Comparative real-time PCR and enzyme analysis of selected gender-associated molecules inSchistosoma japonicum

Lactate dehydrogenase and malate dehydrogenase: Potential antiparasitic targets for drug development studies

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