Lactation transcriptomics in the Australian marsupial, Macropus eugenii: transcript sequencing and quantification

Lefèvre, Christophe; Digby, Matthew R.; Whitley, Jane C.; Strahm, Yvan; Nicholas, Kevin R.

doi:10.1186/1471-2164-8-417

Cited by 54 publications

(64 citation statements)

References 46 publications

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“…Important whey protein components such as immunoglobulin genes are not reported in the present figure. Expression of beta-lactoglobulin was only reported for the Kangaroo [93]. The relative mRNA abundance between the caseins, LALBA, and ALB is also reported.…”

Section: Figurementioning

confidence: 99%

“…In Figure 1 are reported the temporal patterns of transcription of caseins, alpha-lactalbumin, whey acid protein, and albumin from pregnancy to lactation in two ruminant species (dairy cow [89] and dairy goat [90]), two monogastric species (mouse [91] and pig [92]), and a marsupial (kangaroo; Macropus eugenii [93]). Those were the only microarray datasets publicly available at the time of this writing.…”

Section: Mrna Abundance and Expression Patterns Of Transcripts Codingmentioning

confidence: 99%

“…Temporal expression pattern of genes encoding casein alpha-S1, casein alpha-S2 (for mouse the genes are casein alpha s2-like A and casein alpha s2-like B), casein beta, casein kappa (CSN1S1, CSN1S2-(a) and (b) for mouse -, CSN2, and CSN3, respectively), alpha-lactalbumin (LALBA) and albumin (ALB) in dairy cows [89], mouse [91], pig [92], goat [90], and kangaroo (Macropus eugenii; [93]). Dataset, except for dairy cows and kangaroo, was obtained through NCBI Gene Expression Omnibus (GEO at http://www.ncbi.nlm.nih.gov/geo/): GSE14008 for goat, GSE4222 for mouse, and GSE30704 for pig.…”

Section: Figurementioning

confidence: 99%

“…Data from the Series Matrix File were used. A statistical analysis using a false discovery rate for the overall time effect was run using GeneSpring GX7.. Data for the kangaroo are from [93]; only one isoform of alpha casein was reported. Important whey protein components such as immunoglobulin genes are not reported in the present figure.…”

Section: Figurementioning

confidence: 99%

See 3 more Smart Citations

Milk Protein Synthesis in the Lactating Mammary Gland: Insights from Transcriptomics Analyses

Bionaz¹,

Hurley²,

Loor³

2012

Milk Protein

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Section: Figurementioning

confidence: 99%

Section: Mrna Abundance and Expression Patterns Of Transcripts Codingmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Section: Figurementioning

confidence: 99%

See 2 more Smart Citations

Milk Protein Synthesis in the Lactating Mammary Gland: Insights from Transcriptomics Analyses

Bionaz¹,

Hurley²,

Loor³

2012

Milk Protein

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“…Indeed, until recently there had been no studies of the central molecular components of the innate immune system in any marsupials. With recent developments in marsupial biology, experimental strategies to address these issues using molecular approaches are now possible (Daly et al, 2007a;Daly et al, 2007b;Lefevre et al, 2007;Belov et al, 2007;Daly et al, 2008a;Daly et al, 2008b).…”

Section: Introductionmentioning

confidence: 99%

CD14 and TLR4 are expressed early in tammar (Macropus eugenii)neonate development

Daly

Lefèvre

Nicholas

et al. 2008

Journal of Experimental Biology

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SUMMARYMarsupials are born in a relatively underdeveloped state and develop during a period of intensive maturation in the postnatal period. During this period, the young marsupial lacks a competent immune system, but manages to survive despite the potential of exposure to environmental pathogens. Passive immune transfer via the milk is one well-recognised strategy to compensate the neonate, but there also may be innate immune mechanisms in place. In this study, CD14 and Toll-like receptor 4 (TLR4), integral molecular components of pathogen recognition, were identified and characterised for the first time in a marsupial, the tammar wallaby (Macropus eugenii). Functional motifs of tammar CD14 and the toll/interleukin receptor (TIR) domain of TLR4 were highly conserved. The lipopolysaccharide (LPS) binding residues and the TLR4 interaction site of CD14 were conserved in all marsupials. The TIR signalling domain had 84% identity within marsupials and 77% with eutherians. Stimulation of adult tammar leukocytes resulted in the induction of a biphasic pattern of CD14 and TLR4 expression, and coincided with increased production of the pro-inflammatory cytokine TNF-α. Differential patterns of expression of CD14 and TLR4 were observed in tammar pouch young early in development, suggesting that early maturation of the innate immune system in these animals may have developed as an immune survival strategy to protect the marsupial neonate from exposure to microbial pathogens.

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Genome Sequence of an Australian Kangaroo,Macropus eugenii

Deakin

2013

Encyclopedia of Life Sciences

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The tammar wallaby ( Macropus eugenii ) is a model marsupial species that has been intensively used for research into various aspects of marsupial biology. Recently, it became one of only three marsupial species to have its genome sequenced. Comparisons of genome sequence from all three marsupials with those of other vertebrates have made it possible to begin identifying marsupial‐specific genome features. Our knowledge of the biology of the tammar wallaby has informed the interpretation of these unique features and provided insight into their evolution. Among these unique features are genes involved in the sophisticated marsupial lactation system and genes encoding for potent antimicrobial peptides. Comparisons of genome organisation have also provided insight into marsupial genome evolution. Key Concepts: Uncovering the genetic basis of unique marsupial features is now possible due to the availability of genome sequences for three marsupial species. The tammar wallaby is a model species used for studies into marsupial biology, making it possible to link genomic features with the biological characteristics. As the tammar wallaby genome was only lightly sequenced, other genomic resources such as transcriptome sequences and genome maps have enhanced the genome assembly. A number of novel genes are associated with the complex marsupial lactation system and immunity. Five novel genes have been identified on the marsupial Y chromosome. The organisation of the tammar wallaby major histocompatibility complex is vastly different to that of any other species studied to date. A decades old debate over chromosome number in the ancestral marsupial has been resolved by comparing genome organisation among the sequenced marsupials species and the outgroup species human and chicken. The wallaby genome assembly in combination with other genomic resources are proving valuable for the discovery of marsupial‐specific features.

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Lactation transcriptomics in the Australian marsupial, Macropus eugenii: transcript sequencing and quantification

Cited by 54 publications

References 46 publications

Milk Protein Synthesis in the Lactating Mammary Gland: Insights from Transcriptomics Analyses

Milk Protein Synthesis in the Lactating Mammary Gland: Insights from Transcriptomics Analyses

CD14 and TLR4 are expressed early in tammar (Macropus eugenii)neonate development

Genome Sequence of an Australian Kangaroo,Macropus eugenii

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