Lactic Acid Production from Old Oil Palm Trunk Sap in the Open Batch, Open Repeated Batch, Fed-Batch, and Repeated Fed-Batch Fermentation by Lactobacillus rhamnosus ATCC 10863

Abstract

The cost of fermentable sugars added as a substrate is one major problem for economic lactic acid (LA) production. Old oil palm trunks (OPT) squeezed sap, the agricultural wastes on replanting and pruning of oil palm (Elaeis guineensis), contained mainly glucose and fructose as a potential feedstock to use as a vast carbon source for LA production. To improve the LA yield and productivity, various fermentation modes were performed by Lactobacillus rhamnosus ATCC 10863 using OPT sap as a basal medium. A modified constant feed mode of fed-batch and repeated fed-batch fermentation using undiluted OPT sap feed medium can achieve a high average LA concentration of 95.94 g/L, yield of 1.04 g/g, and productivity of 6.40 g/L/h) at 11 h cultivation time. It can also provide open and open repeated batch fermentation with an average LA concentration of 91.30 g/L, yield of 0.87 g/g, and productivity of 3.88 g/L/h at 21 h fermentation time.

Circular Economy Approaches in the Palm Oil Industry: Enhancing Profitability through Waste Reduction and Product Diversification

“…Saelee et al investigated cost-effective lactic acid production using old OPT sap as a substrate [159]. They employed various fermentation modes with Lactobacillus rhamnosus ATCC 10863.…”

Underutilized Malaysian Agro-Industrial Wastes as Sustainable Carbon Sources for Lactic Acid Production

“…Similarly, Saelee [55] examined the use of Lactobacillus rhamnosus ATCC 10863 and different fermentation modes to boost lactic acid yield and productivity using undiluted OPT sap. The modified constant feed mode surpassed the repeated open batch fermentation, resulting in higher lactic acid yield (1.04 g/g), productivity (6.40 g/L/h), and a shorter fermentation time (11 h).…”