Lacticaseibacillus absianus sp. nov., isolated from the cecum of a mini-pig

Abstract

A rod-shaped, facultative anaerobic, Gram-stain-positive bacteria, isolated from the cecum of a mini-pig, was designated as strain YH-lac23T. Analysis of 16S rRNA gene sequences revealed that the strain was closely related to Lacticaseibacillus daqingensis JCM 33273T (97.9 %), Lacticaseibacillus porcinae KCTC 21027T (96.2 %) and Lacticaseibacillus manihotivorans KCTC 21010T (95.7 %). Analysis of housekeeping gene sequences (pheS and recA) revealed that the strain formed a sub-cluster with L. daqingensis . The average nucleotide identity value for YH-lac23T and its most closely related strain ( L. daqingensis ) is 80.7 %. The main fatty acids are C18 : 1ω9c and C16 : 0. The cell wall contains the peptidoglycan of meso-diaminopimelic acid. The G+C content of the genomic DNA is 59.8 mol%. In view of the chemotaxonomic, phenotypic and phylogenetic properties, YH-lac23T (=KCTC 25006=JCM 33998) represents a novel taxon. The name Lacticaseibacillus absianus sp. nov. is proposed.

Lacticaseibacillus kribbianus sp. nov., isolated from pig farm faeces dump

“…Isolate YH-lac21 T produced two types of lactic acid: d-(1 %) and l-lactate (99 %), which is similar to L. absianus KCTC 25006 T [5] and L. nasuensis KCTC 15317 T [4]. The dominant whole-cell fatty acids (mean value >10 % of whole fatty acids) of the isolate were…”

“…Isolate YH-lac21 T produced two types of lactic acid: d - (1 %) and l -lactate (99 %), which is similar to L. absianus KCTC 25006 T [5] and L. nasuensis KCTC 15317 T [4].…”

“…Lacticaseibacilli are Gram-stain-positive, rod-shaped, non-spore-forming, facultative anaerobes that generally grow at temperatures between 10 and 45 °C [2]. The G+C content of Lacticaseibacillus ranges from 47.2 to 59.8 mol % [4,5]. Some members of Lacticaseibacillus, including Lacticaseibacillus rhamnosus GG, L. casei Lc-01 and L. casei Shirota, act as probiotics [6][7][8].…”

“…Some members of Lacticaseibacillus, including Lacticaseibacillus rhamnosus GG, L. casei Lc-01 and L. casei Shirota, act as probiotics [6][7][8]. Species within this genus are isolated from various environments, including human and animal faeces [9,10], animal intestine [5], pickles [11], cheese [12], milk [13], silage [14], sewage [15] and fermentation products [16,17]. Increasing research into the faecal microbiome has revealed novel species of Lacticaseibacillus in animal faeces; examples include Lacticaseibacillus pantheris [18], Lacticaseibacillus chiayiensis [19], Lacticaseibacillus brantae [10] and Lacticaseibacillus saniviri [9].…”

Lacticaseibacillus parakribbianus sp. nov., isolated from a pig farm faeces dump

“…The polar lipid results showed that the isolate contained different polar lipids and shared similar major polar lipid compounds (DPG, PG and APL) with other related species of Lacticaseibacillus. Cell-wall peptidoglycan analysis revealed that the isolate did not contain mesodiaminopimelic acid (m-DAP) as the diagnostic amino acid, which was different from L. absianus KCTC 25006 T [7].…”