Lactobacilli Have a Niche in the Human Nose

Boeck, Ilke De; Broek, Marianne F.L. van den; Allonsius, Camille Nina; Spacova, Irina; Wittouck, Stijn; Martens, Katleen; Wuyts, Sander; Cauwenberghs, Eline; Jokicevic, Katarina; Vandenheuvel, Dieter; Eilers, Tom; Lemarcq, Michelle; Rudder, Charlotte De; Thys, Sofie; Timmermans, Jean‐Pierre; Vroegop, Anneclaire V.; Verplaetse, Alex; Wiele, Tom Van de; Kiekens, Filip; Hellings, Peter; Vanderveken, Olivier M.; Lebeer, Sarah

doi:10.1016/j.celrep.2020.107674

Cited by 83 publications

(81 citation statements)

References 79 publications

(89 reference statements)

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“…Throughout the experiment, pro-inflammatory IL-8 release remained in the range of sterile controls in similar set-ups 70,71 , or on Calu-3 monolayers treated with AMBR2 41 , and was six-to tenfold lower than a similar set-up treated with 10 7 Staphylococcus aureus cells mL −171 . The introduction of AMBR2 in the dual and triple co-culture systems elicited an increase in IL-8 release.…”

Section: Discussionmentioning

confidence: 86%

“…While AMBR2 colonisation in the microbial background of donor 3 was reduced compared to AMBR2 alone or in presence of donor 7 in the simplified screening set-up, this did not occur in the more complex dual and triple co-culture set-ups, despite a relative abundance of 0.29% unclassified Enterobacteriaceae in the inoculum of donor 3. De Boeck et al 41 showed that AMBR2 has superior adaptive properties for survival in the URT, such as fimbriae for attachment, and the www.nature.com/scientificreports/ ability to cope with oxidative stress through the presence of catalase genes. The dual and triple co-culture setups mimic the microenvironment of sinonasal cavities more closely than a submerged monolayer in terms of nutrient availability 75 , host response 76 , and growth surface 61,62 .…”

Section: Discussionmentioning

confidence: 99%

“…Their mechanisms of action can be divided in the exclusion or inhibition of pathogens, improvement of epithelial barrier function, and modulation of local or systemic host immune responses 34 . De Boeck et al 41 have demonstrated that certain lactobacilli are reduced in the nasopharynx of CRS patients compared to healthy controls. One particular Lacticaseibacillus casei strain, the species formerly known as Lactobacillus casei 42 , strain AMBR2, was isolated from the URT of a healthy volunteer.…”

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confidence: 99%

“…These adaptive traits that enable survival and colonisation in the human URT could potentially increase the efficacy of L. casei AMBR2 as live biotherapeutic product. Indeed, an explorative first in-human study showed that the strain could persist in the nose of healthy volunteers up to 2 weeks after the last administration, thus longer than the nasal clearance time of 15 min 41 , but a detailed study on the colonisation dynamics of this strain has not yet been performed.…”

mentioning

confidence: 99%

“…The screening and efficacy testing of potential LBPs for the respiratory tract generally starts in cell culture models as pure strains 41 and 43 , and/or in simple competition experiments with pathogenic species 40,41,44,45 . However, one of the first biological barriers for the successful engraftment of an LBP is formed by the resident microbiota 46 .…”

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confidence: 99%

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Lacticaseibacillus casei AMBR2 modulates the epithelial barrier function and immune response in a donor-derived nasal microbiota manner

Rudder

García‐Timermans

Boeck

et al. 2020

Sci Rep

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Live biotherapeutic products (LBP) are emerging as alternative treatment strategies for chronic rhinosinusitis. The selection of interesting candidate LBPs often involves model systems that do not include the polymicrobial background (i.e. the host microbiota) in which they will be introduced. Here, we performed a screening in a simplified model system of upper respiratory epithelium to assess the effect of nasal microbiota composition on the ability to attach and grow of a potential LBP, Lacticaseibacillus casei AMBR2, in this polymicrobial background. After selecting the most permissive and least permissive donor, L. casei AMBR2 colonisation in their respective polymicrobial backgrounds was assessed in more physiologically relevant model systems. We examined cytotoxicity, epithelial barrier function, and cytokine secretion, as well as bacterial cell density and phenotypic diversity in differentiated airway epithelium based models, with or without macrophage-like cells. L. casei AMBR2 could colonize in the presence of both selected donor microbiota and increased epithelial barrier resistance in presence of donor-derived nasal bacteria, as well as anti-inflammatory cytokine secretion in the presence of macrophage-like cells. This study highlights the potential of L. casei AMBR2 as LBP and the necessity to employ physiologically relevant model systems to investigate host–microbe interaction in LBP research.

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Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Lacticaseibacillus casei AMBR2 modulates the epithelial barrier function and immune response in a donor-derived nasal microbiota manner

Rudder

García‐Timermans

Boeck

et al. 2020

Sci Rep

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Respiratory syndrome coronavirus‐2 response: Microbiota as lactobacilli could make the difference

Zrelli

Amairia

Zrelli

2020

Journal of Medical Virology

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Coronavirus disease 2019 (COVID‐19) is caused by respiratory syndrome coronavirus qualified as SARS‐CoV‐2. Viral penetration requires binding of the viral spike (S) protein to a specific cellular receptor (ACE2) highly expressed in a nasal goblet and ciliated cells. In several countries, the COVID‐19 evolution was relatively benign compared to others and despite noncompliance with health recommendations on several occasions. In this overview, we attempt to define the criteria that could explain such a difference. Among these criteria, the specificity of Lactobacillus genus strains, as a part of nasal microbiota, could play a role of a barrier against viral penetration and could strengthen the host's immune system in some populations rather than others. In fact, several studies have shown the role of lactic acid bacteria, including lactobacilli, in the prevention of viral respiratory infections. This could provide important information on a possible mechanism of the virus spreading.

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Antibiotic Sensitivity and Nasal Microbiome in Patients with Acute Bacterial Rhinosinusitis

et al. 2023

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ObjectivesAcute rhinosinusitis (ARS) is a common upper respiratory tract infection that is mostly of viral origin. However, little is known about the nasal microbiome profile at presentation and the changes caused by antibiotics in acute bacterial rhinosinusitis (ABRS).MethodsThis was a prospective single‐center study. Overall, 43 ARS patients were screened and were assessed with the symptom questionnaires, nasal endoscopy, and Water's view. Five healthy subjects were recruited as controls. Middle meatal mucus samples were obtained using a cotton swab (for bacterial culture and antimicrobial susceptibility testing) and the suction technique (for 16S rRNA sequencing). After 1 week of antibiotic use (amoxicillin with clavulanic acid), we enrolled 13 patients with ABRS with positive isolates and middle meatal samples for 16S rRNA sequencing were obtained again.ResultsOverall, we demonstrated a significantly lower abundance of the Lactobacillaceae family in ABRS patients than in healthy controls. Resistant ABRS had different characteristics of middle meatal microbiomes when compared to sensitive ABRS as follows: (1) lower proportion of lactic acid bacteria, (2) increased pathogens such as Rhodococcus sp., Massila sp., Acinetobacter sp., and H. influenza, and (3) increased beta diversity. However, no remarkable changes were observed in the middle meatal microbiome after antibiotic use.ConclusionWe showed the roles of Lactobacillaceae in ABRS, and Acinetobacter and Massilia in case of amoxicillin resistance.Level of Evidence3 Laryngoscope, 2023

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Lactobacilli Have a Niche in the Human Nose

Cited by 83 publications

References 79 publications

Lacticaseibacillus casei AMBR2 modulates the epithelial barrier function and immune response in a donor-derived nasal microbiota manner

Lacticaseibacillus casei AMBR2 modulates the epithelial barrier function and immune response in a donor-derived nasal microbiota manner

Respiratory syndrome coronavirus‐2 response: Microbiota as lactobacilli could make the difference

Antibiotic Sensitivity and Nasal Microbiome in Patients with Acute Bacterial Rhinosinusitis

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