Lactobacillus casei displaying Clostridium perfringens NetB antigen protects chickens against necrotic enteritis

Shamshirgaran, Mohammad Ali; Golchin, Mehdi; Mohammadi, Elham

doi:10.1007/s00253-022-12155-y

Cited by 10 publications

(8 citation statements)

References 45 publications

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“…Additionally, both intestinal permeability and NE lesion scores showed that the EM-2 group was statistically similar to the NC group. The recurring results with reduced NE lesion scores in groups including EB are similar to those of several previous studies that showed recombinant NetB immunization reduced NE lesion scores in chickens infected with NE ( Jang et al, 2012 ; Keyburn et al, 2013a , b ; Shamshirgaran et al, 2022 ). To date, there are no reports that show dietary effects of IgY antibodies affecting intestinal permeability.…”

Section: Discussionsupporting

confidence: 88%

Hyperimmune egg yolk antibodies developed against Clostridium perfringens antigens protect against necrotic enteritis

et al. 2023

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Section: Discussionsupporting

confidence: 88%

Hyperimmune egg yolk antibodies developed against Clostridium perfringens antigens protect against necrotic enteritis

et al. 2023

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“…However, many harmful bacterial (including Clostridium perfringens and Escherichia coli etc.) at above normal level also had a negative effect on gut microbiota [ 1 , 18 , 62 , 63 ]. Our study results indicated that BA40 had the same ability as PB6 to modulate the balance of gut microbiota, decreasing the population of Clostridium perfringens and Escherichia coli, and increasing the proliferation of Lactobacillus .…”

Section: Discussionmentioning

confidence: 99%

“…Probiotics can interact with gut microbiota and improve the microbial barrier function [ 14 ]. Besides, many bacterial genera have been described as probiotic properties with anti-pathogenic activity, such as bacillus and lactobacillus [ 15 , 16 , 17 , 18 ]. Now, some bacterial of the host’s gut community, such as many bacillus and lactobacillus strains, have been good probiotic candidates.…”

Section: Introductionmentioning

confidence: 99%

Screening of Bacteria Inhibiting Clostridium perfringens and Assessment of Their Beneficial Effects In Vitro and In Vivo with Whole Genome Sequencing Analysis

Jiang

Yang

et al. 2022

Microorganisms

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Various countries and organizations call for banning the use of antibiotic growth promoters (AGPs) as prophylaxis and for growth promotion in the livestock industry. Hence, seeking a substitute for antibiotics is strongly required by the livestock industry to maintain the productivity level and profits. Probiotics could represent one viable solution because of their beneficial effects on host health and maintaining the intestinal microbiota balance. In the present study, we aimed to isolate bacterial strains with probiotics properties from JinHua pig (a Chinese native pig breed) gastrointestinal tract that have antagonistic activity against to common disease-causing bacteria on farms. The four most potent strains were isolated (PP31, BA11, BA40, BV5) by the agar well diffusion method and further characterized by acid, bile salt, trypsin tolerance, whole genome sequencing (WGS), and suppressing Clostridium perfringens adhesion to IPEC-J2 cells. According to these results, BA40 had the highest number and variety of probiotic secondary metabolic secretion genes and capacity to exclude the attachment of Clostridium perfringens to IPEC-J2 cells as same as PB6. The animal experiment in vivo illustrated that BA40 and PB6 could reduce the phenomenon induced by Clostridium perfringens challenge of body weight loss, colon length decrease, pro-inflammatory cytokine increase, and Clostridium perfringens and Escherichia coli increase. The present study provides evidence that BA40 could represent a novel probiotic candidate as PB6, which exhibited some probiotic features and mitigated the burden of Clostridium perfringens associated gut disease.

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“…The L. casei strain, as a safe probiotic bacterium, expresses NetB protein, thus making it a safe vaccine candidate against NE. In the study of [ 185 ], probiotics enhanced the serum anti-NetB antibody responses to NetB protein, and the resultant effect of better weight gain was notable. Synbiotics (PoultryStar me) consisting of various probiotic strains were found to enhance anti-CP IgA and decrease CP load in the guts of birds exposed to NE [ 137 ].…”

Section: Ameliorative Effect Of Probiotics Supplementation On Necroti...mentioning

confidence: 99%

Recent Trends on Mitigative Effect of Probiotics on Oxidative-Stress-Induced Gut Dysfunction in Broilers under Necrotic Enteritis Challenge: A Review

et al. 2023

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Gut health includes normal intestinal physiology, complete intestinal epithelial barrier, efficient immune response, sustained inflammatory balance, healthy microbiota, high nutrient absorption efficiency, nutrient metabolism, and energy balance. One of the diseases that causes severe economic losses to farmers is necrotic enteritis, which occurs primarily in the gut and is associated with high mortality rate. Necrotic enteritis (NE) primarily damages the intestinal mucosa, thereby inducing intestinal inflammation and high immune response which diverts nutrients and energy needed for growth to response mediated effects. In the era of antibiotic ban, dietary interventions like microbial therapy (probiotics) to reduce inflammation, paracellular permeability, and promote gut homeostasis may be the best way to reduce broiler production losses. The current review highlights the severity effects of NE; intestinal inflammation, gut lesions, alteration of gut microbiota balance, cell apoptosis, reduced growth performance, and death. These negative effects are consequences of; disrupted intestinal barrier function and villi development, altered expression of tight junction proteins and protein structure, increased translocation of endotoxins and excessive stimulation of proinflammatory cytokines. We further explored the mechanisms by which probiotics mitigate NE challenge and restore the gut integrity of birds under disease stress; synthesis of metabolites and bacteriocins, competitive exclusion of pathogens, upregulation of tight junction proteins and adhesion molecules, increased secretion of intestinal secretory immunoglobulins and enzymes, reduction in pro-inflammatory cytokines and immune response and the increased production of anti-inflammatory cytokines and immune boost via the modulation of the TLR/NF-ĸ pathway. Furthermore, increased beneficial microbes in the gut microbiome improve nutrient utilization, host immunity, and energy metabolism. Probiotics along with biosecurity measures could mitigate the adverse effects of NE in broiler production.

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Lactobacillus casei displaying Clostridium perfringens NetB antigen protects chickens against necrotic enteritis

Cited by 10 publications

References 45 publications

Hyperimmune egg yolk antibodies developed against Clostridium perfringens antigens protect against necrotic enteritis

Hyperimmune egg yolk antibodies developed against Clostridium perfringens antigens protect against necrotic enteritis

Screening of Bacteria Inhibiting Clostridium perfringens and Assessment of Their Beneficial Effects In Vitro and In Vivo with Whole Genome Sequencing Analysis

Recent Trends on Mitigative Effect of Probiotics on Oxidative-Stress-Induced Gut Dysfunction in Broilers under Necrotic Enteritis Challenge: A Review

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