Lactobacillus spp. and Enterococcus faecium strains isolation, identification, preservation and quantitative determinations from turkey gut content

Sorescu, Ionut; Nutrition, Balotesti; Dumitru, Mihaela; Ciurescu, Georgeta

doi:10.25083/rbl/24.1/41.49

Cited by 14 publications

(32 citation statements)

References 8 publications

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“…The intestinal content (ileum from 1 cm distal to Meckel's diverticulum to the ileo-cecal junction, and cecum) was aseptically removed and placed in sterile plastic bags on ice. Then, in the lab, 10-fold serial dilutions of 1 g of digesta (ileal and cecal content) were homogenized with 7 mL Brain Heart Infusion broth (Oxoid Ltd., England) supplemented with 2 mL glycerol, and immediately frozen at −20°C until the analysis, according to the technique previously described by Sorescu et al. (2019) .…”

Section: Methodsmentioning

confidence: 99%

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Effect of Bacillus subtilis on growth performance, bone mineralization, and bacterial population of broilers fed with different protein sources

et al. 2020

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The objective of this study was to investigate the impacts of Bacillus subtilis ( BS ), ATCC 6051a strain, as a probiotic bacterium in broiler diets based of 2 protein sources (soybean meal [ SBM ] and cowpea seeds [ CWP ]), on growth performance ( GP ), carcass traits, bone mineralization, and microflora population (0 to 42 d age). The SBM and CWP starter, grower, and finisher diets were tested in the presence or absence of BS (5.0 × 10 11 CFU spores g −1 feed) in a 2 × 2 factorial arrangement of treatments in a completely randomized design. Broilers were randomly assigned to 4 dietary treatments with 6 replicate pens per treatment (20 chicks per pen). The results showed that broilers fed CWP had comparable GP (body weight gain, feed intake, and feed conversion ratio) to the birds fed the SBM diet. Carcass, breast and legs' yield, organ size (i.e., gizzard, liver, pancreas, small intestine, cecum), and bone development were not affected by the protein source. The addition of BS in both types of diet improved BWG ( P < 0.001) and feed efficiency, especially in the grower and finisher period ( P = 0.047; P = 0.043 , respectively). In addition, BS significantly decreased abdominal fat ( P = 0.026) and cecum weight ( P = 0.034) and increased tibia bone P concentration ( P = 0.015). Furthermore, BS decrease cecal pH ( P = 0.010) and reduced Escherichia coli and Staphylococcus spp. from cecum and excreta broilers ( P < 0.001; P < 0.0001 , respectively). It is concluded that the BS significantly improved the GP of broilers and can beneficially affect the gut and excreta bacterial community in both SBM and CWP diets.

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Section: Methodsmentioning

confidence: 99%

“…were enumerated on Slanetz-Bartley agar (Oxoid CM0377) incubated at 37°C for 48 h in anaerobic conditions, according to the method of Mountzouris et al. (2007) , modified by Sorescu et al. (2019) .…”

Section: Methodsmentioning

confidence: 99%

Effect of Bacillus subtilis on growth performance, bone mineralization, and bacterial population of broilers fed with different protein sources

et al. 2020

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“…The Mountzouris method (2007) completed by Sorescu et al, (2019) was applied. Sample preparation: 1 g intestinal content (ileum and cecum, respectively) per capita from seventeen chicks (Cobb 500, 45-day-old) was homogenized with 7 ml Oxoid BHI (Brain Heart Infusion) broth and 2 ml glycerol, and immediately frozen at -20 ºC until testing (no more than three months).…”

Section: Lactic Acid Bacteria Isolation and Determination Of Total Bacterial Countmentioning

confidence: 99%

“…After defrost, decimal dilutions from every sample were inoculated on Oxoid Man, Rogosa, Sharpe (MRS) agar. Further instead, the procedure presented in other paper (Sorescu et al, 2019) for the isolation and counting of Lactobacillus CFU has been applied.…”

Section: Lactic Acid Bacteria Isolation and Determination Of Total Bacterial Countmentioning

confidence: 99%

Lactobacillus spp. Strains Isolation, Identification, Preservation and Quantitative Determinations from Gut Content of 45-Day-Old Chickens Broilers

Sorescu

Dumitru

Ciurescu

2021

Braz. J. Poult. Sci.

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The objective of this study was to isolate, identify, preserve and determine the quantitative level of the Lactobacillus strains from the gut content of 45-day-old chickens broilers; to test the viability of these strains preserved at 4 ºC and room temperature (20 ± 2 ºC). Lactobacillus strains were isolated, phenotypically identified and preserved from the gut content of 17 chickens broilers. Identification was performed by morphological, cultural and biochemical characters examination, using apiwebTM and ABIS online software. The quantitative level of Lactobacillus strains in intestinal content (10 5 -10 9 CFU/g) and the viability of strains preserved at 4 ºC and at room temperature (from 8 days to 9 months) was also determined. Twenty-three strains of L. acidophilus, L. brevis, L. plantarum, L. fermentum and L. salivarius from the gut content of chickens broilers were isolated, phenotypically identified, and preserved. Of these, L. plantarum, L. fermentum and L. acidophilus biotype 1 strains were technologically and ecologically suitable to continue the testing of probiotic traits.

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“…However, there are some molecular differences between mammalian IgG and bird IgY ( Table 1). The evolutionary distance between birds and mammals has made these two molecules have different properties [21,22]. For example, unlike IgG, IgY lacks a hinge region between the two "arms" of the antibody (Figure 1).…”

Section: Molecular Properties Of Igymentioning

confidence: 99%

Can Immunization of Hens Provide Oral-Based Therapeutics against COVID-19?

et al. 2020

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In the current worldwide pandemic situation caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) and the newest coronavirus disease (COVID-19), therapeutics and prophylactics are urgently needed for a large population. Some of the prophylaxis strategies are based on the development of antibodies targeting viral proteins. IgY antibodies are a type of immunoglobulin present in birds, amphibians, and reptiles. They are usually obtained from egg yolk of hyper-immunized hens and represent a relatively inexpensive source of antibodies. Specific IgY can be produced by immunizing chickens with the target antigen and then purifying from the egg yolk. Chicken IgY has been widely explored as a clinical anti-infective material for prophylaxis, preventive medicine, and therapy of infectious diseases. Administered non-systemically, IgY antibodies are safe and effective drugs. Moreover, passive immunization with avian antibodies could become an effective alternative therapy, as these can be obtained relatively simply, cost-efficiently, and produced on a large scale. Here, we highlight the potential use of polyclonal avian IgY antibodies as an oral prophylactic treatment for respiratory viral diseases, such as COVID-19, for which no vaccine is yet available.

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Lactobacillus spp. and Enterococcus faecium strains isolation, identification, preservation and quantitative determinations from turkey gut content

Cited by 14 publications

References 8 publications

Effect of Bacillus subtilis on growth performance, bone mineralization, and bacterial population of broilers fed with different protein sources

Effect of Bacillus subtilis on growth performance, bone mineralization, and bacterial population of broilers fed with different protein sources

Lactobacillus spp. Strains Isolation, Identification, Preservation and Quantitative Determinations from Gut Content of 45-Day-Old Chickens Broilers

Can Immunization of Hens Provide Oral-Based Therapeutics against COVID-19?

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