Lactococcus Lactis Strains From Intestinal Organ of Black Tips Shark Carcharhinus Limbatus Producing Nisin-Like Bacteriocin Active Against Shrimp and Fish Pathogens (Vibrio Parahaemolyticus and Vibrio Alginolyticus)

Hamid, Tengku Aizan; Zahli, Khairil Ikhwan Ahmad; Mohammed, Alotaibi

doi:10.15414/jmbfs.2020.10.3.354-360

Cited by 5 publications

(8 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nevertheless, this observation is consistent with many observations that LAB inhibits Gram negative bacteria [21]. In one of our previous work, strain L. lactis strain isolated from black tip shark was able to produce a potent antimicrobial compound, bacteriocin [22]. With this antimicrobial action, for example, strain H-Hn has potential to be used in the Pangasius aquaculture.…”

Section: Antimicrobial Propertiessupporting

confidence: 89%

The Co-Isolation of Lactic Acid Bacteria (LAB) and A Related Pathogenic Strain from Pangasius Nasutus

HAJA MOHIDEEN,

ALİAS,

MANSUR

et al. 2023

International Journal of Life Sciences and Biotechnology

View full text Add to dashboard Cite

Catfish species Pangasius nasutus, or locally known as Patin Buah is one of a popular aquaculture product in Malaysia. Probiotic strain is an attractive alternative to conventional antibiotics in managing aquaculture diseases. Pangasius can be a source of bacterial strain in developing suitable probiotic useful in Pangasius rearing. This study highlights on the isolation of LAB strains from the selected tissues from P. nasutus which are the heart, stomach, and intestines. These strains were subjected to morphological, biochemical, and genotypic characterisations. Three different strains were isolated from fish tissues, H-Hn from heart; S-Hn from stomach; and I-Sk from intestine. Biochemical characterisation were consistent with ribosomal rRNA sequencing, in which strain H-Hn (acc. No. MW504962) is highly similar (100%) to Lactococcus lactis, and both I-Sk (MW504964) and S-Hn (MW504963) are similar (99%) to Lactococcus garvieae. All strains appeared to be non-spore forming, non-motile, Gram-positive coccus, catalase and citrate negative, and fermenting sugar. Unlike H-Hn, both H-Sk and I-Sk strains were able to grow at 6.5% NaCl and at higher temperature (45°C). The antimicrobial properties were assessed using agar disk diffusion assay against several indicator organisms (Escherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium, Staphylococcus aureus and Vibrio parahaemolyticus). These strains exhibited varying degree of inhibitory properties against the indicator organisms. Strain H-Hn inhibited E. coli and V. parahaemolyticus. Meanwhile, strains I-Sk and S-Hn showed a rather broad-spectrum inhibition. From P. nasutus, other L. lactis strain, a pathogenic species from L. garvieae could also be found especially in their gut tissues. The presence of L. garvieae in commercial P. nasutus should raise some concern to those who like to consume this fish. Meanwhile, the LAB strains isolated has probiotics potential that can be commercially used in the managing fish diseases in aquaculture.

show abstract

Section: Antimicrobial Propertiessupporting

confidence: 89%

The Co-Isolation of Lactic Acid Bacteria (LAB) and A Related Pathogenic Strain from Pangasius Nasutus

HAJA MOHIDEEN,

ALİAS,

MANSUR

et al. 2023

International Journal of Life Sciences and Biotechnology

View full text Add to dashboard Cite

show abstract

“…The two microbial strains used in this work; 1) the previously isolated Lactococcus lactis strain FA1 (Genbank: MN975529.1) used as probiotics strain for shrimp feeding (Hamid et al, 2020); and 2) pathogenic strain Vibrio parahaemolyticus used to elicit EMS disease on shrimp. These strains were retrieved from bacterial stock collection available at Kulliyah of Science, International Islamic university Malaysia, Indera Mahkota Campus.…”

Section: Bacterial Strainsmentioning

confidence: 99%

“…Now, the use of this bacterium was made broadened beyond just in dairy industries; into genetic engineering and microbial cell factory (Zielińska and Kolożyn-Krajewska, 2018). In our previous work, a few strains of Lactococcus lactis strains were successfully isolated from the intestine of Black tip shark (Hamid et al, 2020). These novel strains were able to inhibit Vibrio parahaemolyticus, the pathogenic strain which has elicited Early Mortality Syndrome (EMS) in shrimp.…”

Section: Introduction Introduction Introduction Introductionmentioning

confidence: 99%

The effect of supplementation of Lactococcus lactis strain as probiotic on the growth and survival of Litopenaeus vannamei

BAHARUDDIN,

GHANI,

OTHMAN

et al. 2024

Not Sci Biol

View full text Add to dashboard Cite

Acute Hepatopancreatic Necrosis Disease (AHPND) or Early Mortality Syndrome (EMS) is a bacterial disease in shrimps caused by pathogen Vibrio parahaemolyticus. It has culminated in huge loss in global shrimp production due to mass mortality. A probiotic strain Lactococcus lactis strain FA1 was recently isolated from shark intestine, showing inhibition towards the growth of the pathogen. Due to inhibitory potential, the effect of probiotics strain on growth performance of shrimp infected with V. parahaemolyticus was evaluated. The probiotic strain was incorporated into feed for juvenile shrimps Litopanaeus vannamei for 3 weeks before which they were then challenged with pathogen Vibro parahaemolyticus. The study compares 4 shrimp groups: Control (Without any treatment); Group A (Probiotic treated, uninfected); Group B (Probiotic treated, infected) and Group C (No probiotic, infected). The survival and growth performance (weight and length gain) of shrimps were evaluated in the following 30 days. Statistical analyses (ANOVA; Post Hoc Tukey) were used to compare between shrimp groups. In general, infected shrimp demonstrated some of the key symptoms of AHPND (pale or white hepatopancreas), transparent body and erratic swimming behaviour. The supplementation of probiotics resulted in an improved survivability (65±1%) compared to infected shrimp (45±1%). The probiotic treated shrimp group showed to have better % body weight gain, in which weight gain between group B (treated/infected) and group C (untreated/infected) were significantly different (at P=0.046); and so, to group A (treated/uninfected) when compared with group C (P=0.047). There is however no significant difference in % length gain between the groups. Meanwhile, some of the infected shrimps were able to show recovery from the infection. Pre-infection probiotic treatment showed better performance compared to post-infection probiotic treatment. This indicated that the strain Lactococcus lactis is highly suitable for use as the future probiotic in shrimp aquaculture.

show abstract

“…In the same way, L. lactis isolated from the marine black tips shark inhibited a wide range of fish pathogens. 32 L. lactis is known to produce bacteriocin J46, Lacticin 481, Nisin and their derivatives, Lactococcin and their derivatives that enable them to suppress a wide range of fish pathogens. The Bacteriocin-like substances (BLIS) secreted by lactic acid bacteria (LAB) isolated from freshwater crayfish gut was effective in mitigating the A. hydrophila infection in crayfish.…”

Section: Appli C Ati On S Of Bac Teri O Cin S In Aquaculture Sys Temsmentioning

confidence: 99%

“…In the same way, L . lactis isolated from the marine black tips shark inhibited a wide range of fish pathogens 32 . L .…”

Section: Applications Of Bacteriocins In Aquaculture Systemsmentioning

confidence: 99%

Potential application of bacteriocins for sustainable aquaculture

Nayak

Karunasagar

Chakraborty

et al. 2021

Reviews in Aquaculture

View full text Add to dashboard Cite

The emergence and prevalence of bacterial pathogens in freshwater aquaculture systems is a growing concern as the economic losses associated with diseases are mounting and impacting the fishery sector. One way of controlling fish mortality and morbidity is using bacteriocin‐secreting probiotics, which are generally considered safe without any adverse effects that usually accompany other treatment strategies. Understanding the mode of action of bacteriocin on pathogens is vital for considering them suitable for application as a prophylactic in aquaculture. Although several measures such as antibiotic treatment, chemotherapy, vaccination, etc., are available, though not without concerns, these methods have limitations for field‐level applications since they result in several adverse effects both for the animals and the aquatic environment affecting several species in the food chain. In this situation, the use of bacteriocin can be an excellent alternative to minimize the disease burden in freshwater aquaculture if delivered appropriately. Here, we provide an update on the various bacteriocins and their mode of action against important pathogens encountered in aquaculture.

show abstract

Lactococcus Lactis Strains From Intestinal Organ of Black Tips Shark Carcharhinus Limbatus Producing Nisin-Like Bacteriocin Active Against Shrimp and Fish Pathogens (Vibrio Parahaemolyticus and Vibrio Alginolyticus)

Cited by 5 publications

References 24 publications

The Co-Isolation of Lactic Acid Bacteria (LAB) and A Related Pathogenic Strain from Pangasius Nasutus

The Co-Isolation of Lactic Acid Bacteria (LAB) and A Related Pathogenic Strain from Pangasius Nasutus

The effect of supplementation of Lactococcus lactis strain as probiotic on the growth and survival of Litopenaeus vannamei

Potential application of bacteriocins for sustainable aquaculture

Contact Info

Product

Resources

About