Lactoferrin Augmentation of the BCG Vaccine Leads to Increased Pulmonary Integrity

Hwang, Shen-An; Welsh, Kerry J.; Kruzel, Marian L.; Actor, Jeffrey K.

doi:10.1155/2011/835410

Cited by 18 publications

(30 citation statements)

References 79 publications

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“…The adaptive immune response is dominated by T-cell activity, which includes various functions. T-helper cell type 1 (Th1) and type 2 (Th2) stimulate and activate Mf, resulting in intracellular killing events that eliminate intracellular pathogens [77] . Lf can downregulate allergic rhinitis, which reduces inflammatory responses, because it upregulates the expression of Th2, Th17 and regulatory T cells.…”

Section: T Lymphoc Ytesmentioning

confidence: 99%

Immunomodulatory effects of lactoferrin

et al. 2014

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Lactoferrin (Lf) is an iron-binding glycoprotein of the transferrin family, which is expressed in most biological fluids with particularly high levels in mammalian milk. Its multiple activities lie in its capacity to bind iron and to interact with the molecular and cellular components of hosts and pathogens. Lf can bind and sequester lipopolysaccharides, thus preventing pro-inflammatory pathway activation, sepsis and tissue damages. Lf is also considered a cell-secreted mediator that bridges the innate and adaptive immune responses. In the recent years much has been learned about the mechanisms by which Lf exerts its activities. This review summarizes the recent advances in understanding the mechanisms underlying the multifunctional roles of Lf, and provides a future perspective on its potential prophylactic and therapeutic applications.

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Section: T Lymphoc Ytesmentioning

confidence: 99%

Immunomodulatory effects of lactoferrin

et al. 2014

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“…It is also able to modulate cell growth rate and to bind viral glycosaminoglycans and bacterial lipopolysaccharides (Wakabayashi et al, 2006). Its use as vaccine adjuvant modulates the adaptive immune response in humans (Hwang et al, 2011). Moreover, recombinant Lf has been tested for clinical use in treatment and prevention of such human and animal diseases as solid tumors (Hayes et al, 2005(Hayes et al, , 2010 and diarrhea (Humphrey et al, 2002).…”

Section: Characteristics Of the Key Functions Of Lactoferrinmentioning

confidence: 99%

“…Moreover, recombinant Lf has been tested for clinical use in treatment and prevention of such human and animal diseases as solid tumors (Hayes et al, 2005(Hayes et al, , 2010 and diarrhea (Humphrey et al, 2002). Its use as vaccine adjuvant modulates the adaptive immune response in humans (Hwang et al, 2011).…”

Section: Characteristics Of the Key Functions Of Lactoferrinmentioning

confidence: 99%

Plant‐based biopharming of recombinant human lactoferrin

Yemets

Tanasienko

Krasylenko

et al. 2014

Cell Biology International

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Recombinant proteins are currently recognized as pharmaceuticals, enzymes, food constituents, nutritional additives, antibodies and other valuable products for industry, healthcare, research, and everyday life. Lactoferrin (Lf), one of the promising human milk proteins, occupies the expanding biotechnological food market niche due to its important versatile properties. Lf shows antiviral, antimicrobial, antiprotozoal and antioxidant activities, modulates cell growth rate, binds glycosaminoglycans and lipopolysaccharides, and also inputs into the innate/specific immune responses. Development of highly efficient human recombinant Lf expression systems employing yeasts, filamentous fungi and undoubtedly higher plants as bioreactors for the large-scale Lf production is a biotechnological challenge. This review highlights the advantages and disadvantages of the existing non-animal Lf expression systems from the standpoint of protein yield and its biological activity. Special emphasis is put on the benefits of monocot plant system for Lf expression and the biosafety aspects of the transgenic Lf-expressing plants.

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“…In a highly activated inflammatory environment, such as under lipopolysaccharide (LPS) stimulation, the addition of lactoferrin actively decreased macrophage production of inflammatory cytokines TNF-α and IL-12 (Hwang et al 2007a; Doursout et al 2013). During low antigen stimulation, such as vaccination with the TB vaccine BCG (Bacillus Calmette Guerin), the addition of lactoferrin enhanced vaccine efficacy by increasing the antigen recall IFN-γ response (Hwang et al 2005, 2007b, 2009a, 2009b, 2011a, 2011b; Hwang and Actor 2009), which is necessary for the control of MTB infection. Based on published data, it was hypothesized that oral lactoferrin could decrease the pathology of MTB-induced lung inflammation while preserving the cytokine environment required for the control of MTB organisms.…”

Section: Introductionmentioning

confidence: 99%

Oral recombinant human or mouse lactoferrin reducesMycobacterium tuberculosisTDM induced granulomatous lung pathology

Hwang

Kruzel

Actor

2017

Biochem. Cell Biol.

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Trehalose 6'6-dimycolate (TDM) is the most abundant glycolipid on the cell wall of Mycobacterium tuberculosis (MTB). TDM is capable of inducing granulomatous pathology in mouse models that resembles those induced by MTB infection. Using the acute TDM model, this work investigates the effect of recombinant human and mouse lactoferrin to reduce granulomatous pathology. C57BL/6 mice were injected intravenously with TDM at a dose of 25 μg-mouse-1. At day 4 and 6, recombinant human or mouse lactoferrin (1 mg · (100 μL)−1 mouse−1) were delivered by gavage. At day 7 after TDM injection, mice were evaluated for lung pathology, cytokine production, and leukocyte populations. Mice given human or mouse lactoferrin had reduced production of IL-12p40 in their lungs. Mouse lactoferrin increased IL-6 and KC (CXCL1) in lung tissue. Increased numbers of macrophages were observed in TDM-injected mice given human or mouse lactoferrin. Granulomatous pathology, composed of mainly migrated leukocytes, was visually reduced in mice that received human or mouse lactoferrin. Quantitation of granulomatous pathology demonstrated a significant decrease in mice given human or mouse lactoferrin compared with TDM control mice. This report is the first to directly compare the immune modulatory effects of both heterologous recombinant human and homologous mouse lactoferrin on the development of TDM-induced granulomas.

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Lactoferrin Augmentation of the BCG Vaccine Leads to Increased Pulmonary Integrity

Cited by 18 publications

References 79 publications

Immunomodulatory effects of lactoferrin

Immunomodulatory effects of lactoferrin

Plant‐based biopharming of recombinant human lactoferrin

Oral recombinant human or mouse lactoferrin reducesMycobacterium tuberculosisTDM induced granulomatous lung pathology

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