Lactoferrin inhibits the inflammatory and angiogenic activation of bovine aortic endothelial cells

Yeom, Mijung; Park, Jongbong; Lee, Bombi; Choi, Seo‐Hyun; Kim, Kyoung Soo; Lee, Hyejung; Hahm, Dae Hyun

doi:10.1007/s00011-010-0294-1

Cited by 28 publications

(23 citation statements)

References 45 publications

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“…5 Furthermore, bLF has been shown to suppress angiogenic activation in bovine aortic ECs. 7 Thus, the effects of LF on angiogenesis remain controversial, although the results of this study indicate that bLF activates endothelial function and promotes an ischemia-induced angiogenic response. Although it is difficult to explain why our results are in opposition to those of previous studies, 1 plausible reason is that the doses of bLF used were different, ranging from 0.01 to 1 mg/mL in our study versus 10 to .100 mg/mL in others.…”

Section: Discussionmentioning

confidence: 72%

Bovine Milk–derived Lactoferrin Exerts Proangiogenic Effects in an Src-Akt-eNOS–dependent Manner in Response to Ischemia

Ikeda¹,

Tajima²,

Izawa‐Ishizawa³

et al. 2013

Journal of Cardiovascular Pharmacology

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Lactoferrin (LF) exerts a variety of biological effects, including the promotion of angiogenesis by increasing the expression of angiogenesis-related genes and reducing blood pressure via a nitric oxide-dependent mechanism. In this study, we investigated the effects of LF on angiogenesis using C57BL/6J mice that received daily unilateral treatment with or without bovine milk-derived LF (bLF) after unilateral hindlimb surgery. The analysis of laser speckle blood flow showed that bLF treatment promoted blood flow recovery in response to ischemic hindlimb. The capillary density of ischemic adductor muscles and the phosphorylation of Src, Akt, and endothelial nitric oxide synthase (eNOS) were also significantly higher in bLF-treated mice than in vehicle-treated mice. Furthermore, bLF increased the phosphorylation levels of Src, Akt, and eNOS in in vitro experiments using human aortic endothelial cells. The action of bLF on eNOS phosphorylation was abolished by both LY294002, a phosphatidylinositol 3-kinase inhibitor, and 4-amino-5-(4-chlorophenyl)-7-(dimethylethyl)pyrazolo [3,4-d]pyrimidine (PP2), an Src inhibitor. Similarly, bLF-induced acceleration of tube formation, cell proliferation, and cell migration in human aortic endothelial cells were inhibited by LY294002 or PP2. Thus, bLF promotes vascular endothelial cell function via an Src Akt eNOS-dependent pathway, thereby contributing to revascularization in response to ischemia.

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Section: Discussionmentioning

confidence: 72%

Bovine Milk–derived Lactoferrin Exerts Proangiogenic Effects in an Src-Akt-eNOS–dependent Manner in Response to Ischemia

Ikeda¹,

Tajima²,

Izawa‐Ishizawa³

et al. 2013

Journal of Cardiovascular Pharmacology

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“…Cells were then transferred to 96 well plates coated with Matrigel Basement Membrane Matrix (LDEV-free, Corning) in triplicate and cultured in EBM media (Lonza). Formation of tubes by human microvascular endothelial cells was examined 18hrs later using HMVECs cultured in Matrigel as described [23]. The tube number and tube length per well were counted using NIS Elements software (Nikon).…”

Section: Methodsmentioning

confidence: 99%

TNFα contributes to diabetes impaired angiogenesis in fracture healing

Lim

Mattos

et al. 2017

Bone

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Diabetes increases the likelihood of fracture, interferes with fracture healing and impairs angiogenesis. The latter may be significant due to the critical nature of angiogenesis in fracture healing. Although it is known that diabetes interferes with angiogenesis the mechanisms remain poorly defined. We examined fracture healing in normoglycemic and streptozotocin-induced diabetic mice and quantified the degree of angiogenesis with antibodies to three different vascular markers, CD34, CD31 and Factor VIII. The role of diabetes-enhanced inflammation was investigated by treatment of the TNFα-specific inhibitor, pegsunercept starting 10 days after induction of fractures. Diabetes decreased both angiogenesis and VEGFA expression. The reduced angiogenesis and VEGFA expression in diabetic fractures was rescued by specific inhibition of TNF in vivo. In addition, the TNF inhibitor rescued the negative effect of diabetes on endothelial cell proliferation and endothelial cell apoptosis. The effect of TNFα in vitro was enhanced by high glucose and an advanced glycation endproduct to impair microvascular cell proliferation and stimulate apoptosis. The effect of TNF, high glucose and an AGE was mediated by the transcription factor FOXO1, which increased expression of p21 and caspase-3. These studies indicate that inflammation plays a major role in diabetes-impaired angiogenesis in endochondral bone formation through its effect on microvascular endothelial cells and FOXO1.

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“…IL-1B increases the activity of hypoxia-inducible factor (HIF)-1, which promotes the production and release of VEGF. 16,17 It is thought that the inhibition of these cytokines occurs via LF interference with nuclear transcription factor B (NF-kB). 18 Oral administration of bovine LF in mice increases levels of IL-18, an anti-angiogenic cytokine with possible direct action on endothelial cells.…”

Section: Introductionmentioning

confidence: 99%

Lactoferrin Reduces Chorioretinal Damage in the Murine Laser Model of Choroidal Neovascularization

Montezuma

Dolezal

Rageh

et al. 2015

Current Eye Research

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Lactoferrin inhibits the inflammatory and angiogenic activation of bovine aortic endothelial cells

Abstract: Lf exerted a potent effect on BAEC activation, suggesting that it might function via an endothelia-based mechanism in the treatment of various diseases, including rheumatoid arthritis and cancer.

Cited by 28 publications

References 45 publications

Bovine Milk–derived Lactoferrin Exerts Proangiogenic Effects in an Src-Akt-eNOS–dependent Manner in Response to Ischemia

Bovine Milk–derived Lactoferrin Exerts Proangiogenic Effects in an Src-Akt-eNOS–dependent Manner in Response to Ischemia

TNFα contributes to diabetes impaired angiogenesis in fracture healing

Lactoferrin Reduces Chorioretinal Damage in the Murine Laser Model of Choroidal Neovascularization

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