Lactoferrin promotes MC3T3-E1 osteoblast cells proliferation via MAPK signaling pathways

Liu, Meng; Fan, Fan; Shi, Peipei; Tu, Maolin; Yu, Chang Yeon; Yu, Chenxu; Du, Ming

doi:10.1016/j.ijbiomac.2017.08.151

Cited by 67 publications

(53 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The present data collectively suggested that GP‐12 enhanced osteoblastic proliferation by inducing an alteration to the cell‐cycle progression. This result was similar to that of lactoferrin and kobophenol A …”

Section: Resultssupporting

confidence: 86%

“…This result was similar to that of lactoferrin and kobophenol A. 24,36 Effect of GP-12 on mRNA and protein expression of the genes involved in osteoblastic proliferation and differentiation Cyclin D1 plays an essential role during cell cycle regulation, which has proven to enhance the growth and proliferation of osteoblasts. 14 Runx 2 and Osterix are two major transcription factors that can specifically activate osteoblastic differentiation and mineralization, which plays a critical role in the maturation, morphology and function of osteocytes during bone formation.…”

Section: Effect Of Gp-12 On Cell Cycle Distribution Of Osteoblastssupporting

confidence: 76%

“…For the proliferation and survival assays, 200 μL of cell mediums were seeded into 96‐well plates, and the assays were conducted three times at each concentration (0, 0.02, and 0.05 mg mL −1 GP‐12). The proliferation of osteoblasts was measured by the Methyl Thiazolyl Tetrazolium (MTT) method after incubating for 48 h. The distribution of cell cycles was analyzed by Flow Cytometry (CytoFLEX, Beckman Coulter, Germany) …”

Section: Methodsmentioning

confidence: 99%

“…The proliferation of osteoblasts was measured by the Methyl Thiazolyl Tetrazolium (MTT) method after incubating for 48 h. The distribution of cell cycles was analyzed by Flow Cytometry (CytoFLEX, Beckman Coulter, Germany). 24 Quantitative real-time polymerase chain reaction (PCR) analysis Total RNA was extracted from MC3T3-E1 cells treated with GP-12 (0, 0.02, 0.05 mg mL −1 ) and XAV-939 (0.05 mg mL −1 GP-12 + 20 μmol L −1 XAV-939) using TRIzol reagent according to the manufacturer's protocol. Then RNA (2.0 μg) was reversetranscribed to cDNA with oligo (dT) primers from the PrimScript cDNA Synthesis Kit.…”

Section: Materials and Reagentsmentioning

confidence: 99%

See 3 more Smart Citations

Yak (Bos grunniens) bones collagen‐derived peptides stimulate osteoblastic proliferation and differentiation via the activation of Wnt/β‐catenin signaling pathway

Zhang

Zhu

et al. 2020

J Sci Food Agric

View full text Add to dashboard Cite

BACKGROUND As the world's population is transitioning gradually to an ageing stage, the incidence of osteoporosis is increasing annually. Yak bone is one of the major components of Tibetan medicine and it has mainly been associated with an improvement in bone health, for example against osteoporosis. However, the functional bioactive ingredients and the underlying mechanisms are still unclear. RESULTS Sequential purification of yak‐bone hydrolysates was achieved by ultrafiltration, size exclusion chromatography, and semi‐preparative reverse‐phase high‐performance liquid chromatography. After this, 35 novel peptides were identified by mass spectrometry analysis, of which peptide GPAGPPGPIGNV (GP‐12) displayed the highest osteoblast proliferation‐promoting activity, with an increase of 42.7% in cell growth. An in vitro stability study demonstrated that GP‐12 was digested into smaller peptides (GP‐9, GV‐9, AV‐10 and GP‐11) after simulated gastrointestinal digestion and absorption (Caco‐2 cell monolayers) experiments. However, some of them still can be absorbed intact through the (Caco‐2 cell monolayers by a paracellular route (Papp: 5.36 ± 0.34 cm s−1). Flow cytometry results indicated that GP‐12 enhanced osteoblastic proliferation by inducing the alteration of the cell‐cycle progression both from the G0/G1 to the S phase and from the S to the G2/M phase. Quantitative real‐time polymerase chain reaction (PCR) and western blot results revealed that GP‐12 induced osteoblastic proliferation and differentiation in a dose–response manner through the activation of a Wnt/β‐catenin signaling pathway. CONCLUSION These findings highlighted that such peptides hold the promise of discovering candidates for functional and health‐promoting foods, which could be potentially used for the treatment of osteoporosis. © 2020 Society of Chemical Industry

show abstract

Section: Resultssupporting

confidence: 86%

Section: Effect Of Gp-12 On Cell Cycle Distribution Of Osteoblastssupporting

confidence: 76%

Section: Methodsmentioning

confidence: 99%

Section: Materials and Reagentsmentioning

confidence: 99%

See 2 more Smart Citations

Yak (Bos grunniens) bones collagen‐derived peptides stimulate osteoblastic proliferation and differentiation via the activation of Wnt/β‐catenin signaling pathway

Zhang

Zhu

et al. 2020

J Sci Food Agric

View full text Add to dashboard Cite

show abstract

“…P38 is capable of increasing the proliferation of osteoblasts and inhibiting the differentiation of osteoclasts by activating NF-KB, Max, P53, and Stat1 to improve osteoporosis (24). Moreover, P38 is an osteoblast-derived protein with a significant effect on the regulation of cell proliferation (25). Also, ERK has an essential impact on maintaining the balance between bone formation and bone resorption by promoting the differentiation of MSCs to osteoblasts.…”

Section: Discussionmentioning

confidence: 99%

Dioscin improves postmenopausal osteoporosis through inducing bone formation and inhibiting apoptosis in ovariectomized rats

Zhao

et al. 2019

BST

View full text Add to dashboard Cite

Postmenopausal osteoporosis (PMO) has become a public health problem worldwide. Hormonal replacement therapy (HRT) is the most popular treatment for PMO at present, but the side effects, including increased risk of endometrial cancer and breast cancer, limit its clinical use. Therefore, finding a new medication with high efficiency and less side-effects is urgently required. Dioscin is the main ingredient of some medicinal plants such as Dioscorea nipponica Makino and Dioscorea zingiberensis Wrigh. It is reported that dioscin has anti-tumoral and anti-atherosclerotic activity as well as an inhibitory effect on hepatic fibrosis. In this study, the effects of dioscin on PMO were examined and the mechanisms were analyzed. The results indicated that the bone mineral density and ultimate load of PMO rats were increased after being treated with dioscin. H&E staining and immunohistochemical staining showed the bone trabeculae formation and bone differentiation of PMO rats were promoted by dioscin. Western blots revealed that dioscin could activate the PI3K/P38/AKT signaling pathway and inhibit the apoptosis signaling pathway in bone tissue cells of PMO rats. In addition, MTT assays showed that MC3T3-E1 cell viability could be improved by dioscin. These results suggest dioscin is a potential therapeutic reagent for osteoporosis and deserves further investigation.

show abstract

Zoledronate promotes osteoblast differentiation in high‐glucose conditions via the p38MAPK pathway

Hou

Qin

et al. 2022

Cell Biology International

View full text Add to dashboard Cite

Zoledronate (ZOL) were found to inhibit bone resorption in an animal model of diabetes, high glucose concentrations have been shown to decreased the osteogenesis-related gene expression. But the molecular mechanism by which high glucose levels affect osteoblasts and the effects of ZOL on osteoblast differentiation in a high-glucose environment remain unclear. Therefore, we aimed to investigate the effect of ZOL on osteoblast differentiation in a high-glucose environment and determine the responsible mechanism. Cell proliferation was detected by MTT assay, and cell differentiation was evaluated by immunofluorescence staining for alkaline phosphatase expression, alizarin red staining, cytoskeletal arrangement, and actin fiber formation. Real-time PCR and western blot analyses were performed to detect the mRNA and protein expression of p38MAPK, phosphorylated (p)-p38MAPK, CREB, p-CREB, collagen (COL) I, osteoprotegerin (OPG), and RANKL. The results showed that cell proliferation activity did not differ among the groups. But high glucose inhibited osteoblast differentiation; actin fiber formation; and p38MAPK, p-p38MAPK, CREB, p-CREB, COL I, and OPG expression, while promoting RANKL expression. However, we found that treatment with ZOL reversed these effects of high glucose. And further addition of a p38MAPK inhibitor led to inhibition of osteoblast differentiation and actin fiber formation, and lower p38MAPK, p-p38MAPK, CREB, p-CREB, COL I, and OPG expression than in the high glucose +ZOL group with higher RANKL expression than in the high glucose +ZOL group.Collectively, this study demonstrates that high glucose inhibits the differentiation of osteoblasts, and ZOL could partly overcome these effects by regulating p38MAPK pathway activity.

show abstract

Lactoferrin promotes MC3T3-E1 osteoblast cells proliferation via MAPK signaling pathways

Cited by 67 publications

References 40 publications

Yak (Bos grunniens) bones collagen‐derived peptides stimulate osteoblastic proliferation and differentiation via the activation of Wnt/β‐catenin signaling pathway

Yak (Bos grunniens) bones collagen‐derived peptides stimulate osteoblastic proliferation and differentiation via the activation of Wnt/β‐catenin signaling pathway

Dioscin improves postmenopausal osteoporosis through inducing bone formation and inhibiting apoptosis in ovariectomized rats

Zoledronate promotes osteoblast differentiation in high‐glucose conditions via the p38MAPK pathway

Contact Info

Product

Resources

About