Lactosucrose production by various microorganisms harboring levansucrase activity

Abstract

Production of the artificial sweetener, lactosucrose, by various microorganisms containing levansucrase activity was investigated. Of the tested bacteria, Bacillus subtilis was the most effective producer using lactose as an acceptor and sucrose as a fructosyl donor. Lactosucrose production by this strain was optimal at pH 6.0 and 55 degrees C whereupon 181 g lactosucrose l(-1) was produced from 225 g lactose l(-1) and 225 g sucrose l(-1) in 10 h.

Production, characterization and antioxidant activities in vitro of exopolysaccharides from endophytic bacterium Paenibacillus polymyxa EJS-3

“…This is consistent with the finding of many other investigators (Han & Clarke, 1990;Lee et al, 1997). It has been reported that levansucrase with strong sucrose hydrolyzing activity is involved in many P. polymyxa strains, which may be responsible for the high yield of EPS with sucrose as carbon source (Park, Choi, & Oh, 2005).…”

Biosynthesis of lactosylfructoside by an intracellular levansucrase from Bacillus methylotrophicus SK 21.002

“…In other studies on lactosylfructoside production, including levansucrase from P. polymyxa, 17 Sterigmatomyces elviae 18 and other various microorganisms, 19,20,22 the optimum pH values were primarily in the range of 6.0-7.0, whereas it was 4.0 for levansucrase from Pseudomonas aurantiaca. 21 …”

“…The result revealed that the equal concentration of the two substrates showed a better performance than the other concentration ratios, with 400 mg/mL total sugar concentration and a fixed levansucrase dosage at 8 U/g substrates, as shown in Figure 2e. Most studies showed the same result, [17][18][19]22 whereas Han et al 21 reported that the yield of lactosylfructoside formation improved with an increase in the ratio of [sucrose]/[lactose].…”

“…By contrast, the lower optimum temperature for lactosylfructoside production was reported to be 23°C for levansucrase from Zymomonas mobilis, 19 a higher temperature of 45°C was reported for levansucrase from Pseudomonas aurantiaca, 21 and 50°C for levansucrase from Sterigmatomyces elviae 18 and 40-55°C for levansucrase from various microorganisms were reported. 22 …”

“…K-1 [13][14][15] or by levansucrase (EC 2.4.1.10) from a variety of microorganisms, including Aerobacter leavanicum, 16 Paenibacillus polymyxa, 17 Sterigmatomyces elviae, 18 etc. [19][20][21][22] It is known that these enzymes catalyze the transfer and hydrolysis reactions of lactosylfructoside and sucrose. 15,20 The crystal structures of b-fructofuranosidase from Arthrobacter sp.…”