Laminin 332 Is Indispensable for Homeostatic Epidermal Differentiation Programs

Tayem, Raneem; Niemann, Catherin; Pesch, Monika; Morgner, Jessica; Niessen, Carien M.; Wickström, Sara A.; Aumailley, Monique

doi:10.1016/j.jid.2021.04.008

Cited by 17 publications

(9 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(b) A specific molecular network in the DEJ of infant and elderly skin At the molecular level, the DEJ contains a well-organized network of various proteins including laminin 332 (also called laminin V), types IV and VII collagens, heparan sulfate proteoglycan (perlecan) and nidogens. Laminin 332, a ligand of basal keratinocyte integrins, is essential for epidermal attachment in mice and humans (Rousselle et al, 1991(Rousselle et al, , 1997Nakano et al, 2002;Rousselle & Beck, 2013;Tayem et al, 2021). It has been reported to enhance hemidesmosome formation and reinforce the DEJ in skin-equivalent models (Amano, Nishiyama & Burgeson, 1999), but also to regulate the differentiation of mouse and human interfollicular epidermal stem cells which themselves produce laminin 332 (Yamada et al, 2018;Tayem et al, 2021), to inhibit sensory mechanotransduction and thereby decrease pain sensitivity in mice, and to control sensory axon branching behaviour (Chiang et al, 2011).…”

Section: Rasmussenmentioning

confidence: 99%

Newborn and elderly skin: two fragile skins at higher risk of pressure injury

Bengy¹,

Lamartine

Sigaudo‐Roussel

et al. 2021

Biological Reviews

View full text Add to dashboard Cite

Skin is a key organ maintaining internal homeostasis by performing many functions such as water loss prevention, body temperature regulation and protection from noxious substance absorption, microorganism intrusion and physical trauma. Skin ageing has been well studied and it is well known that physiological changes in the elderly result in higher skin fragility favouring the onset of skin diseases. For example, prolonged and/or high‐intensity pressure may suppress local blood flow more easily, disturbing cell metabolism and inducing pressure injury (PI) formation. Pressure injuries (PIs) represent a significant problem worldwide and their prevalence remains too high. A higher PI prevalence is correlated with an elderly population. Newborn skin evolution has been less studied, but some data also report a higher PI prevalence in this population compared to older children, and several authors also consider this skin as physiologically fragile. In this review, we compare the characteristics of newborn and elderly skin in order to determine common features that may explain their fragility, especially regarding PI risk. We show that, despite differences in appearance, they share many common features leading to higher fragility to shear and pressure forces, not only at the structural level but also at the cellular and molecular level and in terms of physiology. Both newborn and elderly skin have: (i) a thinner epidermis; (ii) a thinner dermis containing a less‐resistant collagen network, a higher collagen III:collagen I ratio and less elastin; (iii) a flatter dermal–epidermal junction (DEJ) with lower anchoring systems; and (iv) a thinner hypodermis, resulting in lower mechanical resistance to skin damage when pressure or shear forces are applied. At the molecular level, reduced expression of transforming growth factor β (TGFβ) and its receptor TGFβ receptor II (TβRII) is involved in the decreased production and/or increased degradation of various dermal extracellular matrix (ECM) components. Epidermal fragility also involves a higher skin pH which decreases the activity of key enzymes inducing ceramide deficiency and reduced barrier protection. This seems to be correlated with higher PI prevalence in some situations. Some data also suggest that stratum corneum (SC) dryness, which may disturb cell metabolism, also increases the risk of PI formation. Besides this structural fragility, several skin functions are also less efficient. Low applied pressures induce skin vessel vasodilation via a mechanism called pressure‐induced vasodilation (PIV). Individuals lacking a normal PIV response show an early decrease in cutaneous blood flow in response to the application of very low pressures, reflecting vascular fragility of the skin that increases the risk of ulceration. Due to changes in endothelial function, skin PIV ability decreases during skin ageing, putting it at higher risk of PI formation. In newborns, some data lead us to hypothesize that the nitric oxide (NO) pathway is not fully functional at birth, which m...

show abstract

Section: Rasmussenmentioning

confidence: 99%

Newborn and elderly skin: two fragile skins at higher risk of pressure injury

Bengy¹,

Lamartine

Sigaudo‐Roussel

et al. 2021

Biological Reviews

View full text Add to dashboard Cite

show abstract

“…The LAMA3 gene belongs to the laminin family and is responsive to several epithelial-mesenchymal regulators, including keratinocyte growth factor, epidermal growth factor and skin fibrosis [ 28 ]. Previous studies found that targeted disruption of the LAMA3 gene causes junctional epidermolysis bullosa in many animals [ 29 , 30 , 31 ]. Based on this, we hypothesized that LAMA3 might affect the development of skin hair follicles through these pathways and further regulate cashmere traits.…”

Section: Resultsmentioning

confidence: 99%

Convergent Genomic Signatures of Cashmere Traits: Evidence for Natural and Artificial Selection

Wang

Xie

et al. 2023

IJMS

View full text Add to dashboard Cite

Convergent evolution provides powerful opportunities to investigate the genetic basis of complex traits. The Tibetan antelope (Pantholops hodgsonii) and Siberian ibex (Capra sibirica) belong to different subfamilies in Bovidae, but both have evolved similar superfine cashmere characteristics to meet the cold temperature in plateau environments. The cashmere traits of cashmere goats underwent strong artificial selection, and some traces of domestication also remained in the genome. Hence, we investigated the convergent genomic signatures of cashmere traits between natural and artificial selection. We compared the patterns of convergent molecular evolution between Tibetan antelope and Siberian ibex by testing positively selected genes, rapidly evolving genes and convergent amino acid substitutions. In addition, we analyzed the selected genomic features of cashmere goats under artificial selection using whole-genome resequencing data, and skin transcriptome data of cashmere goats were also used to focus on the genes involved in regulating cashmere traits. We found that molecular convergent events were very rare, but natural and artificial selection genes were convergent enriched in similar functional pathways (e.g., ECM-receptor interaction, focal adhesion, PI3K-Akt signaling pathway) in a variety of gene sets. Type IV collagen family genes (COL4A2, COL4A4, COL4A5, COL6A5, COL6A6) and integrin family genes (ITGA2, ITGA4, ITGA9, ITGB8) may be important candidate genes for cashmere formation and development. Our results provide a comprehensive approach and perspective for exploring cashmere traits and offer a valuable reference for subsequent in-depth research on the molecular mechanisms regulating cashmere development and fineness.

show abstract

“…Within the wound healing process, laminins play dual roles in re-epithelialization and angiogenesis, offering a scaffold for epithelial keratinocytes, facilitating their migration, and reinstating the intact epithelial barrier [30,31]. A surge in LM332 expression (Laminin α3β3γ2) by these keratinocytes is among the initial responses during wound reepithelialization post injury [32]. Healthy oral epithelium interfaces with implant or abutment surfaces, forming structures such as basal plates and hemidesmosomes.…”

Section: Discussionmentioning

confidence: 99%

Anodic oxidation- and hydrogenation-produced TiO₂ nanotubes on titanium and its effect on gingival epithelial cell adhesion ability

Mu,

Wang,

et al. 2024

Phys. Scr.

View full text Add to dashboard Cite

The gingival epithelium plays a crucial role in achieving long-term stability of dental implants, and the hydrogenated TiO2 nanotubes with a superhydrophilic surface exhibit more excellent biological activity than pure titanium implants. However, the effects of the hydrogenated TiO2 nanotubes on human gingival epithelial cells remain unclear. Here, we fabricated hydrogenated TiO2 nanotubes using anodization and hydrogenation to investigate the adhesion of human gingival epithelial cells (HGEs) on structured surfaces in vitro. The topography, roughness, and wettability of three sample types—titanium (Ti), TiO2 nanotubes (TNTs), and hydrogenated TiO2 nanotubes (H2-TNTs)—were characterized. To evaluate cell adhesion, the HGEs were co-cultured with these specimens. This allowed for the examination of both the adhesion morphology and the number of cells adhering to each material's surface. Expression levels of genes and proteins related to cell adhesion were also assessed. H2-TNTs demonstrated nanoscale topography similar to TNTs in terms of diameter and height and maintained a superhydrophilic surface (with a static water contact angle of < 5°). The number of HGEs adhering to H2-TNTs was notably higher. Furthermore, HGEs on H2-TNTs displayed a more stretched morphology in comparison to the other two groups. Notably, the expression levels of adhesion-related genes and proteins in H2-TNTs surpassed those of the other two groups. Hence superhydrophilic H2-TNTs significantly enhance the adhesion ability of HGEs on the material surface.

show abstract

Laminin 332 Is Indispensable for Homeostatic Epidermal Differentiation Programs

Cited by 17 publications

References 58 publications

Newborn and elderly skin: two fragile skins at higher risk of pressure injury

Newborn and elderly skin: two fragile skins at higher risk of pressure injury

Convergent Genomic Signatures of Cashmere Traits: Evidence for Natural and Artificial Selection

Anodic oxidation- and hydrogenation-produced TiO₂ nanotubes on titanium and its effect on gingival epithelial cell adhesion ability

Contact Info

Product

Resources

About

Laminin 332 Is Indispensable for Homeostatic Epidermal Differentiation Programs

Cited by 17 publications

References 58 publications

Newborn and elderly skin: two fragile skins at higher risk of pressure injury

Newborn and elderly skin: two fragile skins at higher risk of pressure injury

Convergent Genomic Signatures of Cashmere Traits: Evidence for Natural and Artificial Selection

Anodic oxidation- and hydrogenation-produced TiO2 nanotubes on titanium and its effect on gingival epithelial cell adhesion ability

Contact Info

Product

Resources

About

Anodic oxidation- and hydrogenation-produced TiO₂ nanotubes on titanium and its effect on gingival epithelial cell adhesion ability