Laminin‐5γ2 chain expression in cervical intraepithelial neoplasia and invasive cervical carcinoma

Noël, Jean‐Christophe; Fernández-Aguilar, Sergio; Fayt, Isabelle; Buxant, Frédéric; Ansion, M.H.; Simon, Philippe; Anaf, Vincent

doi:10.1111/j.0001-6349.2005.00879.x

Cited by 15 publications

(11 citation statements)

References 28 publications

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“…Recent reports proposed a close relationship among FGF2, laminin and CD44. [7][8][9][10]22 This study examined laminin and CD44 were examined in both pretreatment and midtreatment cervical cancer biopsies. The pretreatment samples showed twice as many patients with continuity of laminin staining pattern (Table 2), while discontinuity of staining was increased in midtreatment samples.…”

Section: Resultsmentioning

confidence: 99%

“…We next investigated the prognostic value of FGF2, laminin and CD44, based on previous studies. [7][8][9][10][11][12][13][14][15] Patients with stage IV cancer according to the FIGO classification were excluded from the prognostic analysis. The remaining 28 patients were classified into two groups: good responders (n = 18) and poor responders (n = 10).…”

Section: Resultsmentioning

confidence: 99%

“…[7][8][9][10][11][12][13][14][15] We showed previously by microarray analysis that CRT activates signaling from the extracellular matrix (ECM), and revealed many CRT-responsive genes in pre-and mid-radiotherapy cervical cancer biopsies. 16 These genes included basic fibroblast growth factor-2 (FGF2) and heparanase (HPSE).…”

Section: Introductionmentioning

confidence: 99%

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Chemoradiation-induced expression of fibroblast growth factor-2 and laminin in patients with cervical cancer

Nakawatari¹,

Iwakawa²,

Ohno³

et al. 2007

Cancer Biology & Therapy

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Objective: To investigate the protein expression change of FGF2 in cervical cancers during chemoradiotherapy, as indicated in our previous study using microarray analysis. In addition, we sought to examine the predictive value of such changes in expression for disease failure after chemoradiotherapy.Patients and Methods: Biopsy specimens were obtained from 35 patients with cervical cancers before (pretreatment) and 1 week after initiation (midtreatment) of chemoradiotherapy (CRT) (9 Gy and 40 mg/m 2 of cisplatin). Immunohistochemical studies (IHS) were performed to detect FGF2, laminin and CD44 expression using an automated streptavidin-biotin immunoperoxidase staining system. Positive area proportion (%) of FGF2 and CD44 were analyzed using an image analysis system and laminin staining pattern was scored by continuity of the basement membrane immunopositivity. Patients were defined as good (n = 18) or poor responders (n = 10) based on their two-year disease-free survival.Results: Protein expression of FGF2 in midtreatment samples (mid) was significantly higher than in pretreatment samples (pre). Discontinuity of laminin staining pattern in mid was significantly higher than in pre. Protein expression of CD44 was not significantly different between mid and pre. The ratio change (mid versus pre) of FGF2 expression in poor responders was significantly lower than that in good responders (p < 0.05). The number of cases with discontinuity of laminin staining pattern at pre was significantly increased in the poor responders (p < 0.05). Ratio changes of FGF2 or CD44 expression in mid correlated with laminin staining pattern in pre.Conclusions: Using biopsy specimens from pretreatment and midtreatment cervical cancers, we revealed significant changes in FGF2 protein expression during fractionated radiotherapy with cisplatin. We also found that FGF2 ratio change and laminin discontinuity staining pattern at pretreatment were significantly associated with prognosis. These molecular features might help us to identify patients at high risk of disease failure after CRT.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Chemoradiation-induced expression of fibroblast growth factor-2 and laminin in patients with cervical cancer

Nakawatari¹,

Iwakawa²,

Ohno³

et al. 2007

Cancer Biology & Therapy

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“…For example, in colon carcinoma, urinary bladder carcinoma, colorectal carcinoma, SCC of the oral cavity, tongue, skin, kidney, prostate, lung, vagina, hand and neck, gastric carcinoma, alveolar carcinoma, breast cancer, splenic carcinoma, cervical adenocarcinoma, esophageal carcinoma, adenoid cystic carcinoma, ductal tumor of the pancreas, glioma, clear cell carcinoma of the ovary, and epidermoid anal cancer, cells or tissues adjacent to the tumor front express the laminin γ2 chain or its fragment [48][49][50][51][52][53][54][55][56][57][58][59][60][61][62][63][64][65]. The latter fragment is also known as the laminin γ2 short arm (SA) and includes an epidermal growth factor (EGF)-like domain.…”

Section: Section 2 Part 1: Changes In Laminin-332 Expression In Cancermentioning

confidence: 99%

Laminin-332-Integrin Interaction: A Target For Cancer Therapy?

Tsuruta

Kobayashi²,

Imanishi³

et al. 2008

CMC

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For many years, extracellular matrix (ECM) was considered to function as a tissue support and filler. However, we now know that ECM proteins control many cellular events through their interaction with cell-surface receptors and cytoplasmic signaling pathways. For example, they regulate cell proliferation, cell division, cell adhesion, cell migration, and apoptosis. We focus in this review on a laminin isoform, laminin-332 (formerly termed laminin-5), a major component of the basement membrane (BM) of skin and other epithelial tissues. It is composed of 3 subunits (α3, β3, and γ2) and interacts with at least two integrin receptors expressed by epithelial cells (α3β1 and α6β4 integrin). Mutations in either laminin-332 or integrin α6β4 result in junctional epidermolysis bullosa, a blistering skin disease, while targeting of laminin-332 by autoantibodies in cicatricial pemphigoid leads to dysadhesion of epithelial cells from their underlying connective tissue. Abnormal expression of laminin-332 and its integrin receptors is also a hallmark of certain tumor types and is believed to promote invasion of colon, breast and skin cancer cells. Moreover, there is emerging evidence that laminin-332 and its protease degradation products are not only found at the leading front of several tumors but also likely induce and/or promote tumor cell migration. Thus, in this review, we focus specifically on the role of laminin-332 and its integrin receptors in adhesion, proliferation, and migration/invasion of cancer cells. Finally, we discuss strategies for the development of laminin-332-based antagonists for the treatment of malignant tumors.

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“…The role of laminin-332 in cancer progression is yet to be completely defined; Ln-332 could have different mechanisms of action depending on the type of cancer. Expression of Ln-332 is altered in many cancers: it is increased in colon (Pyke, Romer et al 1994;Pyke, Salo et al 1995), cervical (Noel, Fernandez-Aguilar et al 2005) and oral cancers (Gasparoni, Della Casa et al 2007); on the other hand it is decreased in lung (Akashi, Ito et al 2001) and bladder cancers (Sathyanarayana, Maruyama et al 2004). In the case of prostate cancer expression of Ln-332 is entirely lost (Nagle, Hao et al 1995;Davis, Cress et al 2001;Hao, Jackson et al 2001).…”

Section: Chapter V Discussionmentioning

confidence: 99%

Proteolytic Processing of Laminin-332 by Hepsin and Matriptase and Its Role in Prostate Cancer Progression

Tripathi¹

2011

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Laminin‐5γ2 chain expression in cervical intraepithelial neoplasia and invasive cervical carcinoma

Cited by 15 publications

References 28 publications

Chemoradiation-induced expression of fibroblast growth factor-2 and laminin in patients with cervical cancer

Chemoradiation-induced expression of fibroblast growth factor-2 and laminin in patients with cervical cancer

Laminin-332-Integrin Interaction: A Target For Cancer Therapy?

Proteolytic Processing of Laminin-332 by Hepsin and Matriptase and Its Role in Prostate Cancer Progression

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